基于自适应逆控制技术的连续流粮食干燥系统研究

连续流粮食干燥过程是一个非线性大滞后多变量的过程,要精确控制粮食出口的终水分比较困难。本文设计一种新的连续流粮食干燥过程的控制方法,对连续流粮食干燥过程中的多个影响因素进行研究,分析粮食干燥自控系统在设计时所必须选择的可测干扰、数字变量、控制变量和被控变量等,并且提出基于自适应逆控制技术的连续流粮食干燥系统的离散化模型,主要针对工业控制对象中存在的时变和时滞问题,推导出该连续流粮食干燥控制系统的预测模型。通过实验对实际系统进行验证,结果证明本系统具有较好的鲁棒性和稳定性。     

渗灌对日光温室番茄栽培环境的影响

研究了渗灌和漫灌对日光温室番茄栽培环境及番茄产量的影响。结果表明,渗灌下,土壤密度比漫灌低7.59%,差异极显著;土壤总孔隙度比漫灌高9.72%,差异显著;土壤黏粒和粉粒高于漫灌,而砂粒和细粗砾低于漫灌;观测时段内,渗灌下平均气温、10cm处土壤平均温度较漫灌分别高1.55℃、0.86℃,地表平均温度比漫灌低0.14℃;渗灌下温室空气相对湿度较漫灌低11.86%,以10:00—20:00差值最大;渗灌下番茄产量比漫灌高8.60%,且差异显著。     

Preparation and Identification of Canine Parvovirus NY Strain VP2 Protein Polyclonal Antibody

This study was aimed to prepare canine parvovirus (CPV) VP2 protein polyclonal antibody.The recombinant expression vector pET28a-CPV-VP2 was constructed and transfromed into E.coli BL21 (DE3),the expression of recombinant proteins was induced by IPTG from which the fusion protein was identified by SDS-PAGE.The target protein was purified and emulsify with adjuvant,the prepared immunogen was inoculated into rabbit by subcutaneous injections to prepare of VP2 protein specific polyclonal antibody.The immuno-activity,titers,neutralization titers of the prepared polyclonal antibody were determined by immunoperoxidase monolayer assay (IPMA).The results showed that the expressed recombinant protein VP2 (rVP2) existed in the form of inclusion body with a molecular weight of 72 ku.The prepared polyclonal antibody titer was 1 600 dilution,the virus titer was 10⁷ TCID₅₀/mL,the neutralizing titer was 1∶2 884.The antibodies showed specific reaction with CPV.In conclusion,rVP2 specific polyclonal antibody showed wonderful immunocompetence,specificity and neutralizing activity,providing foundation for the development of genetic vaccine and clinical therapeutic method.     

Study on Expression Difference of IGFBP-5 Gene in Different Tissues of Kazakh and Yanqi Horses

The study aimed to explore the mRNA expression pattern of insulin-like growth factor binding protein-5 (IGFBP-5) gene in different tissues of Kazakh and Yanqi horses.The expression of IGFBP-5 gene in different tissues of heart,liver,spleen,lung,kidney,small intestine,large intestine,cecum,intercostal muscles,longissimus dorsi muscles,brachialis muscle and gluteus in two horses were detected by Real-time quantitative PCR and compared the mRNA expression in the same tissues of two breeds.The results showed that the expression of IGFBP-5 in longissimus dorsi muscle and brachialis muscle of two breeds were significantly higher than other tissues including heart,liver,spleen,lung,kidney,small intestine,large intestine and cecum (P<0.05),and was the lowest in large intestine.The expression of IGFBP-5 in kidney,small intestine,large intestine,cecum,longissimus dorsi muscle and brachialis muscles of Yanqi horse were higher than in the same part of Kazakh horse,and among those in longissimus dorsi muscle and large intestine of Yanqi horse were extremely significantly higher than in the same part of Kazakh horse (P<0.01),and in small intestine and cecum of Yanqi horse were significantly higher than in the same part of Kazakh horse (P<0.05).The test was for further researching the biological function of IGFBP-5 gene,and it could provide a theoretical basis for genetic improvement of production performance of horse in our country.     

Effects of Supplemental Complex Water Soluble Vitamin on Production Performance,Blood Biochemical Parameters and Oxidation Resistance of Dairy Cows

The objective of the study was to investigate the effects of supplemental complex water soluble vitamins on production performance, blood biochemical parameters and oxidative resistance of dairy cows in spring and summer.The experiment was designed by 2×2 factorial randomized blocks design.Eighty healthy dairy cows with similar parity and body weight were randomly divided to four groups, with 7 days of adaptation and 70 d experimental phase.The dairy cows in group Ⅲ and Ⅳ were fed a basal diet supplemented with complex water soluble vitamins in spring.The dairy cows in group Ⅱ and Ⅳ were fed a basal diet supplemented with complex water soluble vitamins in summer.The results showed that the ADFI and daily milk yield of dairy cows receiving water soluble vitamin in spring were extremely significantly higher (P<0.01);The ADFI of dairy cows receiving water soluble vitamin was significantly higher in summer (P<0.05) and daily milk yield were extremely significantly higher (P<0.01);The butterfat rate was extremely significantly increased and SCC was extremely significantly decreased in spring and summer (P<0.01);Supplementation of water soluble vitamin was also significantly increased LYM (P<0.05).In summer, T-AOC, T-SOD were extremely significantly increased (P<0.01), and TP, CK, LDH, TP, HDL and ALB were extremely significantly decreased (P<0.01) by water soluble vitamin supplementation than that of control group.In conclusion, complex water soluble vitamins could increase the production performance and non-specific immune function, improve the oxidative resistance and relieved the heat stress of the dairy cows.     

Isolation and Identification of Pathogenic Bacteria Causing Dairy Cow Mastitis in Liaoning and Drug Sensibility Test of Pathogenic E.coli

In order to understand the main bacterial pathogen species causing dairy cow mastitis in Liaoning, as well as the characteristics of drug sensitivity of the pathogenic E.coli,the milk samples from 75 dairy cows with clinical manifestations for mastitis in certain large-scale dairy farm in Liaoning were collected.The bacteria in milk were cultured and isolated with biochemical methods and in vitro drug sensitivity tests were processed with the isolated E.coli strains.The results showed that the main bacterial pathogen for dairy cow mastitis were E.coli（separation rate 58.7%),S.aureus（64.0%）and S.agalactiae（54.7%),and multiple infection including double and triple infection were identified.The drug sensitivity tests on the isolated E.coli indicated that the E.coli isolates were highly resistant to sulfonamides（resistance rate>85%）and chloramphenicol（resistance rate>30%),and they were relatively low resistant to ampicillin（9.5%),ciprofloxacin（9.5%),ceftiofur（7.1%）and ofloxacin（4.8%).The results was able to provide reliable theoretical basis for prevention and control of dairy cow mastitis in Liaoning area.     

苹果园几种鸟雀驱避技术效果研究

选择种有"美国八号"(早熟)、"嘎啦"(中熟)、"富士"(晚熟)的混栽果园4片(各2/3hm~2)为研究对象,设置4个处理(分别为有色防鸟网、风动叶轮、超声波、生物驱避剂),对比了4种鸟雀驱避技术,调查其驱避效果,以筛选出最优的鸟雀驱避技术。结果表明:在相同品种条件下,4种鸟雀驱避技术防鸟害的有效程度以有色防鸟网驱避效果最好,持效性最长,稳定性最好;其受啄率分别为"美国八号"0.44%、"嘎啦"0.16%、"富士"0.20%。其次为生物驱避剂,其受啄率分别为"美国八号"1.92%、"嘎啦"2.24%、"富士"1.52%。然后为超声波驱避技术,其受啄率分别为"美国八号"9.64%、"嘎啦"5.56%,"富士"3.24%。驱避效果最差、持效性最短、稳定性最差的是风动叶轮驱鸟,其受啄率分别为"美国八号"10.00%、"嘎啦"6.52%、"富士"2.60%。3个品种综合调查结果表明,早熟苹果受啄率最高,其次是中熟品种,受啄率最低的是晚熟品种。     

光温调控对切花菊"优香"养分利用及品质的影响

光照与温度影响着切花菊的花芽分化进程,是切花菊畸形花形成的主要环境因子。如何在夏季高温期有效调控设施内的光照与温度对减少畸形花率、提高产品出成率及保鲜期具有重要意义。现以解决企业关键技术需求为出发点,以夏秋菊品种"优香"为试材,在设施生产条件下,利用温湿度自动监控系统、光温调控设施进行了遮光、降温处理,旨在为高温期的切花菊生产光温调控技术提供支撑。结果表明:采收期遮光与降温处理(8 127kg/hm~2)与未调控处理(6 270kg/hm~2)相比,地上部干物质累积量差异不显著,但切花菊的吸磷量显著增加了7.1kg/hm~2,氮和钾的吸收量差异不显著。经光温调控后,切花菊畸形花率由未调控下的42.5%降低至20.1%,外观品质基本一致,清水瓶插寿命延长了2~3d。基于自动温湿度监控系统的切花菊设施生产光温调控技术对切花菊外观品质影响不大,且在未影响切花菊正常干物质累积及养分利用的前提下,可有效降低花朵畸形花率、提高保鲜期和优级花的产出。     

梨新鲜度与其挥发性成分的关系研究

以砀山酥梨为试材,采用顶空固相微萃取-气相色谱-质谱联用(HS-SPME-GC-MS)技术,分析了砀山酥梨在(4±1)℃条件下挥发性成分的变化,检测了砀山酥梨可溶性固形物、硬度、酸度等新鲜度指标,探讨了砀山酥梨新鲜度与其挥发性成分之间的关系。结果表明:新鲜砀山酥梨在(4±1)℃条件下的保鲜时间为42d,其特征性气味成分主要为正己醇、1-辛醇;不新鲜砀山酥梨的特征性气味成分为2-甲基丁基乙酸酯、硅酸四乙酯、异戊醇、正己醇、1-辛醇、香叶基丙酮,且砀山酥梨由新鲜变为不新鲜时的阈值分别为1.57、2.74、1.39、15.15、211.13、2.36μg/L。试验结果为通过气味判断砀山酥梨的新鲜度提供了理论参考。     

北虫草菌种复壮方法的比较

以已退化的北虫草菌种为试材,采用孢子分离、组织分离、活体蚕蛹、基内菌丝分离、蚕蛹回接等5种菌种复壮法,研究了不同复壮方法对退化菌种复壮效果的影响。结果表明:经活体蚕蛹复壮法得到的菌种在菌丝生长速度和子实体产量均是最高的,菌丝生长速度提高了29.97%,子实体产量提高了383.93%,故确定活体蚕蛹复壮法是5种方法中最好的虫草菌种复壮法。