北京小龙门森林公园野生花卉资源评价与应用分析

为丰富北京园林绿化植物的多样性,通过样地法对北京小龙门国家森林公园野生花卉资源进行了实地调查研究,共调查到野生花卉植物58种,隶属于23科。以花色、花径、花序、花型为评价指标,评价出20种具高观赏价值的野生花卉;对这些野生花卉的观赏性进行了评述,最后提出了合理开发利用的建议,以期为北京园林绿化提供可参考的乡土植物资源。     

辽宁地区奶牛乳房炎细菌性病原分离鉴定及大肠杆菌药敏试验

为了掌握辽宁地区奶牛乳房炎主要细菌性病原种类及致奶牛乳房炎大肠杆菌药物敏感性特征,本研究选取辽宁地区某大型奶牛场75头临床表现为乳房炎的奶样进行细菌培养与分离,通过生化方法对分离到的细菌进行鉴定,并对分离到的奶牛源大肠杆菌进行体外药物敏感性试验研究。结果发现,该场奶牛乳房炎细菌性病原主要为大肠杆菌、葡萄球菌和链球菌,其检出率分别为58.7%、64.0%和54.7%,存在二重感染和三重感染等混合感染的情况。大肠杆菌药物敏感性试验结果表明,该场分离株对磺胺类药物（耐药率>85%）及氯霉素类药物（耐药率>30%）耐药性较高,对氨苄西林（9.5%）、环丙沙星（9.5%）、头孢噻呋（7.1%）和氧氟沙星（4.8%）比较敏感。上述研究结果为该地区奶牛乳房炎的防制提供可靠的理论依据。     

安普霉素对仔猪内分泌的调控作用及血液生化指示的影响

采用单因子试验设计 ,28日龄大长北三元杂交断奶仔猪72头随机分为3组 ,研究饲料中添加不同剂量的安普霉素 (0、20、90mg/kg)对仔猪内分泌的调控作用及血液生化指标的影响。试验期为4周。结果表明 :仔猪日粮中添加90mg/kg的安普霉素可促进机体与生长有关的内分泌活动 ,提高内源激素 (生长激素、胰岛素、甲状腺激素T3)水平 (P<0.05),从而促进肌肉蛋白沉积 ;并具有显著降低血液中氨、尿素氮含量和提高血糖水平的作用 (P<0.05) ,表明安普霉素对仔猪具有增加氮沉积 ,促进蛋白质合成、抑制蛋白质分解的作用     

农作物害虫标本数据库的开发研究

数据库包括鳞翅目、鞘翅目、半翅目、同翅目、双翅目、膜翅目、直翅目、蜱螨目和缨翅目等149种农作物害虫,原色图812幅和害虫防治技术信息。     

Instant effects of temperature on mechanical properties of single living intact human red blood cell

AIM: To study the instant effect of circumstance temperature on mechanical properties of single living intact human red blood cell. METHODS: The shape, size, membrane bending elastic modulus and shear elastic modulus of single living intact red blood cell was determined with non-invasive, in situ, real time by employing a static micro-image analyzing and a dynamic micro-image analyzing system. RESULTS: The contact area and diameter of red blood cells were decreased as the temperature increased from 35 ℃ to 43 ℃. The membrane bending elastic modulus and shear elastic modulus of single living intact red blood cell were the least at physiological temperature 37 ℃. CONCLUSION: Red blood cells are easy to carry out their physiological functions such as carrying oxygen to all of the body with the best shape and mechanical deformation at 37 ℃.     

The latent gene expression of Epstein-Barr virus in nasopharyngeal carcinoma

AIM: To examine the latent membrane protein 1(LMP1)-DNA sequence in nasopharyngeal carcinoma(NPC) and detect mRNA expression of LMP1,EBNA1,EBNA2,and to explore the relationship between EBV infectious status,expression products and NPC carcinogenesis.METHODS: LMP1 DNA was detected in NPC by PCR.Direct sequence was applied to analyze the difference between NPC-LMP1-DNA and B95-8- LMP1-DNA.mRNA expressions of LMP1,EBNA1,EBNA2 in NPC were detected by nested RT-PCR.RESULTS: LMP1 DNA existed in all 47 NPC tissues.Several single nucleotide variations were found between NPC-LMP1-DNA and B95-8- LMP1-DNA.The notable variation was the lost of XhoⅠrestriction site in NPC.Direct sequence showed 30 bp deletion in NPC.The mRNA expressions of LMP1,EBNA1 and EBNA2 in NPC were 76.6%,80.0% and 74.5% respectively by nested RT-PCR.The expression of EBNA1 in NPC was promoted by Q promoter while the expression of EBNA1 in B95-8 was promoted by C promoter.CONCLUSION: The way of EBV involved in NPC is complex.Latent genes such as LMP1,EBNA1 and EBNA2 as well as early lytic gene BARF1 may all play certain roles in NPC carcinogenesis.     

粉纹夜蛾微孢子虫的分离及其初步分类鉴定

从粉纹夜蛾(Trichoplusia ni,Tn)幼虫中分离纯化得到一种微孢子虫,孢子形状为卵圆形,大小为(2.832±0.486)μm×(1.947±0.317)μm。它能寄生粉纹夜蛾的大多数组织器官,在虫体内的发育过程具有微粒子属Nosema的分类特征,并初步命名为Nosema sp.。     

实验性鸡马立克氏病羽髓病变研究

通过光镜1电镜等方法对鸡马立克氏病羽髓组织进行了形态学研究。结果显示，羽髓远端坏死，羽髓中有多量肿瘤细胞浸润，肿瘤细胞呈弥漫性或局部性浸润，电镜观察，肿瘤细胞核异型性明显，核膜凹陷，核内出现假核内包含物，在一些羽髓中，巨噬细胞吞噬肿瘤细胞较多见，而且观察到一些肿瘤细胞发生调亡，结果表明，鸡马立克氏病羽髓病变十分明显，羽髓的形态学变化可用于鸡马立克氏病的诊断。     

农牧交错带小流域防护林水源涵养功能研究

文中以内蒙古赤峰市敖汉旗大五家流域内防护林为研究对象,重点研究该流域内不同防护林涵养水源功能的现状,旨在评价不同植被类型水源涵养功能的差异,同时探寻低功能涵养水源型防护林更新改造途径。研究结果表明:不同防护林的土壤理化性质以及涵养水源功能差异明显,华北落叶松白榆混交、杨树柠条混交枯落物的现存量为3.4695~4.8536 t/hm2,最大持水量为64.225~66.125mm;华北落叶松白榆混交的植被类型具有良好的涵养水源能力,为164.563 t/hm2;灌木纯林和灌木混交林在实际调查中表现较差,在今后的森林经营中应考虑营造针阔混交林。