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991.
H.P. Su C.J. Hou W.H. Chen K.J. Wang Y.H. Chiu H.S. Sun 《Veterinary journal (London, England : 1997)》2013,195(2):254-256
Efficient oral endotracheal intubation of laboratory animals is a challenging technique in veterinary research. This study introduces a miniaturized lighted stylet for rabbit intubation. An experiment with repeated measures on two factors was used to assess the feasibility and efficacy of this method. The first factor compared stylet intubation vs. laryngoscopic intubation. The second compared three practitioners, one with prior experience and two without. Success rates on the initial attempt were not statistically different (χ2 = 2.46, P = 0.12). The time difference between methods was significant (F = 41.007, P < 0.001), although the effect of practitioners was not (F = 1.038, P = 0.365). The mean ± SD of the intubation time, combining results from the three practitioners, was 20.34 ± 17.15 s for the stylet method and 57.58 ± 64.21 s for the laryngoscopic method. The results of this study demonstrate that lighted stylet intubation is efficient, robust, and independent of practitioner experience. 相似文献
992.
以实验生态学和生态工程相结合的措施,按照人为设计理论和生态恢复的一般程序,在中国南方亚热带丘陵地区研究截叶胡枝子(Lespedeza cuneata)草地和宽叶雀稗(Paspalum wettsteinii)草地植被的重建,并探讨了这一生态恢复模式在恢复初期的生态学过程和规律.主要结果如下: 相似文献
993.
高效液相色谱法测定低聚壳聚糖含量 总被引:1,自引:0,他引:1
本实验建立高效液相色谱法测定原料和复合预混料中低聚壳聚糖含量。采用ODSC18柱(150mm×4.6mm,5μm),以磷酸盐缓冲液(pH=2.8~3.0)-乙腈(95+5,V+V)为流动相,流速为1.5mL/min,检测波长为195nm,柱温为35℃。在此色谱条件下,低聚壳聚糖在0.056~0.450mg/mL浓度范围内呈良好线性关系,r=0.9998,方法平均回收率为98.8%,相对标准偏差(RSD)为1.1%(n=9)。本法操作简便、快速、结果准确,可作为低聚壳聚糖含量测定的质控方法。 相似文献
994.
桑蓟马生活习性及防治对策 总被引:3,自引:1,他引:3
桑蓟马(Pseudodendrotrips mori Niwa)俗称举尾虫,属缨翅目(Thysanoptera)、蓟马科(Thripidae)、不完全变态(incomplete metamorphosis)中的渐变态(paurometabola),是我省桑树的主要害虫之一。据调查,全龄使用桑蓟马被害叶与全龄使用正常叶相比,全茧量下降30.0%、茧层量下降37.5%、茧层率下降5.769/6、产卵量下降18.0%、良卵率下降12.0%、孵化率下降8.0%,严重影响蚕农利益及蚕种生产。经多年的调查研究,初步探明了桑蓟马在我场桑园的生活规律及防治对策,现总结于后。 相似文献
995.
996.
Albina E Mesplède A Chenut G Le Potier MF Bourbao G Le Gal S Leforban Y 《Veterinary microbiology》2000,77(1-2):43-57
In early 1992, a CSF epizootic was clinically recognised in a wild boar population of approximately 1300 animals within an area of 250km(2) located in the east of France. In order to check the CSF situation in wild boars outside this area, a serological survey was carried out in the rest of France, for 8 consecutive years (1991-1998). This paper reports on the results obtained during this survey which included wild boars shot during the hunting period but also boars reared within fences. Around 1000-2700 sera a year were tested for the presence of antibodies to classical swine fever virus (CSFV) and also to Aujeszky's disease virus (ADV). Out of 12025 sera tested over the whole period, 80 wild boars were found positive for CSF antibodies. Sixty of them were collected on wild boars shot during the years 1992-1994 in the epizootic area located in east of France and 10 were collected in Corsica during the years 1994-1996. The last four positive samples were single reactors coming from areas or farms, which were thereafter confirmed to be serologically negative. These results together with the fact that no disease has been reported so far illustrate that the French wild boar population is probably not concerned by CSF infection (excepted in the east of France where the disease has now become enzootic). Two hundred and forty nine sera were initially detected as CSF positive but confirmed secondarily as positive for border disease (BD) antibodies. This finding shows that wild boars are also susceptible to infection by ruminant pestiviruses. Four hundred and twenty three wild boars have been found positive for ADV antibodies. In addition, from 1993 to 1995, 909 samples were tested for the presence of antibodies to porcine reproductive and respiratory syndrome virus (PRRSV). Thirty three of them were positive. The results on AD and PRRS antibody detection show that wild boars may constitute a reservoir for various infectious diseases of pigs. 相似文献
997.
Yamasaki M Asano H Otsuka Y Yamato O Tajima M Maede Y 《American journal of veterinary research》2000,61(12):1520-1524
OBJECTIVE: To determine the usefulness of canine RBC with high concentrations of potassium, reduced glutathione (GSH), and amino acid(i.e., HK cells) for in vitro cultivation of Babesia gibsoni. ANIMALS: RBC were obtained from 3 dogs that had inherited HK cells and from 3 genetically unaffected dogs that, therefore, had RBC with lower potassium (LK) concentrations (i.e., LK cells). PROCEDURES: First, B. gibsoni were cultivated using HK or LK cells in alpha-modification of Eagle medium, consisting of Earle salts with glutamine and without ribosides, deoxyribosides, and sodium bicarbonate under a humidified atmosphere containing 5% CO2 at 37 C. Second, parasites were cultivated with LK- or HK-cell lysates. Finally, HK cells were separated into 3 fractions (bottom, middle, top layers) by density gradient centrifugation, and B. gibsoni were cultivated with each of the HK-cell fractions. In addition, the concentrations of free amino acids and reduced glutathione (GSH) in each HK-cell fraction were measured. RESULTS: B. gibsoni preferentially multiplied in HK-cell cultures rather than in LK-cell cultures. Furthermore, the addition of HK-cell lysate to the culture medium resulted in enhanced multiplication of the parasites. Higher multiplication of the parasites was observed in HK cells from the top layer, compared with HK cells from the middle and bottom layers. The HK cells from the top layer had higher concentrations of glutamate, aspartate, and GSH, compared with HK cells from the middle and bottom layer. CONCLUSIONS: Canine HK cells are useful host cells for in vitro cultivation of B. gibsoni, and the high concentrations of glutamate, aspartate, and GSH may result in enhancement of multiplication of the parasites in HK cells. 相似文献
998.
999.
由于蛋种鸡日龄的不同,各器官系统的生长发育和生理功能状态有所不同,对种蛋的受精率、出雏率和健雏率等方面均有一定影响。为了更清楚地了解种鸡的不同产蛋日龄阶段对所产种蛋的受精率,出雏率和健雏率的影响,籍以更好的指导生产实践,提高蛋种鸡种蛋的利用价值,提高种鸡场的经济效益。我们于1999年3月在本单位蛋种鸡场进行了试验,现将试验结果报道如下。 相似文献