全文获取类型
收费全文 | 48125篇 |
免费 | 3100篇 |
国内免费 | 4672篇 |
专业分类
林业 | 3587篇 |
农学 | 2376篇 |
基础科学 | 2156篇 |
5014篇 | |
综合类 | 23223篇 |
农作物 | 3657篇 |
水产渔业 | 2647篇 |
畜牧兽医 | 7327篇 |
园艺 | 3810篇 |
植物保护 | 2100篇 |
出版年
2024年 | 420篇 |
2023年 | 1081篇 |
2022年 | 2283篇 |
2021年 | 2225篇 |
2020年 | 2036篇 |
2019年 | 2015篇 |
2018年 | 1537篇 |
2017年 | 2463篇 |
2016年 | 1585篇 |
2015年 | 2326篇 |
2014年 | 2518篇 |
2013年 | 2775篇 |
2012年 | 4002篇 |
2011年 | 4192篇 |
2010年 | 4083篇 |
2009年 | 3465篇 |
2008年 | 3588篇 |
2007年 | 3266篇 |
2006年 | 2684篇 |
2005年 | 2100篇 |
2004年 | 1395篇 |
2003年 | 898篇 |
2002年 | 869篇 |
2001年 | 842篇 |
2000年 | 744篇 |
1999年 | 264篇 |
1998年 | 35篇 |
1997年 | 16篇 |
1996年 | 25篇 |
1995年 | 24篇 |
1994年 | 15篇 |
1993年 | 17篇 |
1992年 | 17篇 |
1991年 | 14篇 |
1990年 | 10篇 |
1989年 | 3篇 |
1988年 | 1篇 |
1987年 | 11篇 |
1986年 | 10篇 |
1985年 | 2篇 |
1983年 | 1篇 |
1981年 | 3篇 |
1979年 | 1篇 |
1966年 | 2篇 |
1965年 | 1篇 |
1962年 | 9篇 |
1956年 | 20篇 |
1955年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
991.
ZHANG Ying CHENG Hua LUO Zhao-fan XU Ming-tong ZHANG Shao-ling LI Feng YAN Li LI Yan 《园艺学报》2009,25(7):1376-1380
AIM: To evaluate the role of G protein-coupled receptor 40 (GPR40) mediates the effects of free fatty acids (FFAs) on lipoapoptosis in mouse β-cell line NIT-1 and the mechanisms involved in this process.METHODS: NIT-1 cells were supplemented with palmitate (500 μmol/L) or oleate (500 μmol/L) for 48 h, then apoptosis of the cells was determined by the methods of Hoechst 33342, TUNEL and flow cytometry (Annexin V/PI). The small interfering RNA technique was used to inhibit the expression of GPR40 in NIT-1 cell. The mock, control siRNA and GPR40 siRNA transfected cells were either supplemented with palmitate (500 μmol/L) or co-incubated with palmitate and oleate (500 μmol/L for each) for 48 h. The percentages of apoptotic cells were quantified. The expression of p-c-Jun, Bcl-2 and Bax were detected by Western blotting.RESULTS: Palmitate induced β cell lipoapoptosis, whereas oleate inhibited NIT-1 cells from palmitate-induced lipoapoptosis. No significant difference of the percentages of apoptotic cells was indicated among the mock, control siRNA and GPR40 siRNA transfected cells treated with palmitate (P>0.05). However, after co-incubated with palmitate and oleate (500 μmol/L for each) for 48 h, the percentage of apoptotic cells in GPR40 siRNA transfected cells was greater than that in mock (P<0.05), while the expression of p-c-Jun was decreased. The expressions of Bcl-2 and Bax were not affected.CONCLUSION: Palmitate induced β cell lipoapoptosis might not be mediated through GPR40, whereas oleate inhibits NIT-1 cells from palmitate-induced lipoapoptosis, which is mediated at least in part through GPR40, the change of c-Jun expression may play an role in this process, suggesting that GPR40 might be implicated in the control of β cell mass plasticity and GPR40 probably provides a link between obesity and type 2 diabetes. 相似文献
992.
WAN Jian-xin GUO Qi WANG Jing-han PAN Yang-bin CUI Jiong FU Bin-bin XU Yan-fang 《园艺学报》2009,25(8):1628-1634
AIM: To observe the effects of cytokines on renovation of acute renal failure (ARF) in mouse with bone marrow derived mesenchymal stem cell (MSC) transplantation. METHODS: ARF animal model was induced in mouse by subcutaneous injection of cisplatin. Mice were randomly assigned into 3 groups: normal control group, ARF group and MSC group. After 24 h cisplatin injection, animals were injected intravenously with MSC in MSC group. Animals were sacrificed at 1 d, 4 d, 7 d, 14 d and 28 d after cisplatin injection. The blood urea nitrogen (BUN) and serum creatinine (Scr) were measured. The renal morphologic changes were scored with Paller’s criterion on hematoxylin and eosin (HE) stained sections. The mRNA and protein expressions of HGF, BMP-7, TNF-α and IL-10 were detected by RT-PCR and immunohistochemistry method. RESULTS: After 4 d of cisplatin injection, the BUN and Scr values in MSC group were significantly lower than those in ARF group (P<0.01). After 7 d and 14 d, the values of BUN and Scr in MSC group were still lower than those in ARF group (P<0.01, P<0.05). The renal morphologic scores of MSC group were also lower than those of ARF group. After 7 d, the expressions of HGF, BMP-7 and IL-10 were higher in MSC group than those in ARF group, the expression of TNF-α in MSC group was lower than that in ARF group. CONCLUSION: MSC promotes the recovery of acute renal failure induced by cisplatin. The mechanism may partly depend on paracrine of growth factor and amelioration of inflammatory. 相似文献
993.
AIM: To observe the mechanisms of RhoA on vascular reactivity following hemorrhagic shock (HS) in rats. METHODS: The superior mesenteric artery (SMA) in rats subjected to hemorrhagic shock was adopted to assay the vascular reactivity via observing the contraction initiated by norepinephrine (NE) with isolated organ perfusion system. Meanwhile, the effects of Rho kinase, myosin light chain phosphatase (MLCP), myosin light chain kinase (MLCK) on RhoA regulating vascular reactivity were observed. The effects of RhoA agonist U-46619 and inhibitor C3 enzyme on the activities of Rho kianse, MLCP, MLCK and phosphorylation of MLC20 in the vascular smooth muscle cells (VSMC) with hypoxia were also measured. RESULTS: As compared to control group, the cumulative dose-response curves of SMA to NE at 2 h after shock shifted to the right, the maximal contractions (Emax) of NE was significantly decreased. RhoA agonist U-46619 increased the vascular reactivity in the late period of shock. C3 enzyme abolished U-46619 induced the increase in the contractile response of SMA to NE. Rho kinase inhibitor Y-27632 decreased U-46619-induced the increase in the vascular reactivity, MLCP inhibitor calyculin further promoted the increase in the vascular reactivity. However, MLCK inhibitor had no effect on the U-46619-induced change of vascular reactivity. After hypoxia, the activities of Rho kinase and MLCK, and the level of MLC20 phosphorylation were decreased, MLCP activity was increased. RhoA agonist U-46619 increased the activity of Rho kinase and phosphorylation of MLC20, decreased the activity of MLCP, but had no effects on MLCK activity. CONCLUSION: RhoA plays an important role in the regulation of vascular reactivity following shock. The mechanism is closely related to regulating the activities of Rho kinase and MLCP, and increasing the phosphorylation of MLC20 in VSMC. 相似文献
994.
995.
996.
秋播小型大白菜新品种京秋娃娃菜的选育 总被引:2,自引:1,他引:1
京秋娃娃菜是由两个自交不亲和系配制而成的秋播小型大白菜一代杂种。成熟期55 d(天),株型半直立,株高32 cm,开展度36 cm,叶色绿,外叶数8片,球叶数38片;叶球合抱,筒形,球色绿,内叶浅黄色,叶球高24 cm,直径10 cm,球形指数2.4,叶球质量0.6 kg左右,净菜率62.4 %。每667 m2净菜产量约5 000 kg。高抗病毒病,抗霜霉病和黑腐病。适合北京、河北、山东、辽宁、黑龙江、吉林、内蒙古等地秋季栽培,目前累计推广面积400 hm2。 相似文献
997.
998.
999.
1000.