Analysis on Target Genes with Mutation in microRNA of Blue-shelled Chicken and White Leghorn

The study was conducted to explore the potential different characters between Blue-shelled chicken and White leghorn.Global genome microRNA was combined the identified microRNA with complementary lab-predicted microRNA.Then the two breed chicken's SNP data got by GGRS were mapped to the microRNA and focused on SNP that deliberately located in mature-microRNA.Bioinformatics method was adopted for target prediction on microRNA which had SNPs.By further gene enrichment analysis,the study found these genes enriched in 22 GO terms,10 KEGG pathways,and 3 IPA important networks.And they enriched in traits which associated with growth,such as mTOR signaling pathways,Wnt signaling pathways,growth hormone receptor networks and insulin-like growth factor Ⅰ receptor networks.And they also enriched in some laying traits,such as oocyte meiotic signaling pathways and progesterone mature oocytes signaling pathways.The methods and the results might provide references for further studies.     

美国油气管道基本失效概率评估方法及启示

为充分利用油气管道历史失效数据,减少失效概率评估过程中的主观性,提出油气管道基本失效概率的概念及评估方法。对美国管道及危险物品安全管理局数据库的油气管道里程数据、事故数据及失效因素数据进行全面分析,采用基于事故统计的方法评估油气管道基本失效概率。结果表明:美国危险液体管道、输气集气管道、配气管道发生一般事故的基本失效概率分别为1.29次/(10^3 km·a)、2.17次/(10^4 km·a)及4.08次/(10^5 km·a),发生较大事故的基本失效概率分别为4.58次/(10^4 km·a)、1.41次/(10^4 km·a)及2.38次/(10^5 km·a),发生重大事故的基本失效概率分别为9.09次/(10^6 km·a)、9.79次/(10^6 km·a)及1.11次/(10^5 km·a)。美国基本失效概率可作为油气管道失效概率评估的基准线,也可作为风险可接受标准的依据,但不能直接应用于中国管道风险评价,需对其进行修正,建立适用于中国管道的失效数据库。     

产蛋期蛋鸡不同类型玉米净能研究

本试验利用禽用开放式呼吸测热装置进行能量代谢试验,通过间接测热法结合替代法测定不同类型玉米在产蛋期蛋鸡饲粮中的表观代谢能和净能。选用34周龄产蛋期海兰褐蛋鸡180只,随机分为6组,每组30只。试验选用1种玉米-豆粕型基础饲粮和5种待测饲粮。待测饲粮由5种待测玉米(3种2018年10月收获的正常玉米和2种2016年收获储存3年的陈化玉米),分别以50%比例替代基础饲粮构成。试验鸡在舍内笼养,预试期7 d,正试期27 d,其中正试期分为3期,每期9 d(适应3 d、呼吸测热3 d、绝食测热3 d)。每期试验中,从每组中选择4只试验鸡,称重后分别放入呼吸测热装置的12个代谢室(每个代谢室2只),每2个代谢室对应1种饲粮,测定气体交换和排泄物总量,呼吸测热的同时进行消化代谢试验。结果表明:与基础饲粮相比,5种玉米待测饲粮的表观代谢能显著提高(P<0.05),3种正常玉米待测饲粮的净能显著高于基础饲粮和2种陈化玉米待测饲粮(P<0.05);2种陈化玉米的表观代谢能和净能显著低于3种正常玉米(P<0.05)。本试验中,3种正常玉米的表观代谢能分别为16.19、15.85、16.17 MJ/kg,2种陈化玉米的表观代谢能分别为15.12和15.06 MJ/kg;3种正常玉米的净能分别为12.39、12.57、12.25 MJ/kg,2种陈化玉米的净能分别为11.29和12.05 MJ/kg。     

Expression and mechanisms of hsa_circ_087631 in patients with primary biliary cholangitis

AIM To explore the expression and mechanisms of circular RNA hsa_circ_087631 in the patients with primary biliary cholangitis （PBC）. METHODS RT-qPCR was used to detect the expression of hsa_circ_087631 in PBC patients and healthy controls. Hsa-miR-346-overexpressing lentiviral vector pLenti-EF1a-EGFP-F2A-Puro-CMV-MCS was constructed and transfected into human acute T cell leukemia Jurkat cells， and then the expression levels of hsa_circ_087631， Bcl-6 mRNA and interleukin-21 （IL-21） mRNA were detected by RT-qPCR. Dual-luciferase reporter assay was performed to identify the interactions between hsa_circ_087631 and hsa-miR-346. RESULTS The expression of hsa_circ_087631 in the PBC patients was significantly increased compared with the healthy subjects. Hsa-miR-346-overexpressing lentiviral vector pLenti-EF1a-EGFP-F2A-Puro-CMV-MCS was successfully constructed. The expression of hsa-miR-346 was significantly increased after the hsa-miR-346-overexpressing lentiviral vector was transfected into the Jurkat cells， while the expression levels of hsa_circ_0087631， Bcl-6 mRNA and IL-21 mRNA were significantly decreased. After wild-type or mutant hsa_circ_087631 vector and hsa-miR-346 mimics were transfected into 293T cells， the results of dual-luciferase reporter assay showed that hsa-miR-346 significantly decreased the luciferase activity of wild-type hsa_circ_087631 （P<0.01）， but the regulation did not change significantly after mutation of the predicted binding site. CONCLUSION Peripheral blood hsa_circ_087631 level is elevated in the PBC patients. The hsa_circ_087631/hsa-miR-346/Bcl-6 signaling may take effect in human T cells. Hsa-miR-346 significantly reduces the expression of hsa_circ_087631， but it may not be regulated by predicted binding sites.     

困难立地条件下的赣北地区高速公路边坡植物调查及研究

本研究的目的在于调查困难立地条件下的赣北地区10条高速公路边坡植物群落,筛选出边坡乔木层,灌木层、草本层的优势种植物,总结江西省几十年的高速公路边坡植被恢复模式实践经验,将植被恢复情况较好的边坡整理归纳出生态恢复模式,对植被恢复较差的边坡提出针对性和可操作性的优化对策,为后期高速公路边坡植被恢复及养护工作提供参考,以实现江西省高速公路生态效益、社会效益、观赏效益的最大化.     

2011年湖北省鲜食春大豆品种区域试验

2011年征集了14个鲜食大豆品种在湖北武汉、黄冈、黄石、孝感等8个试验点进行区域试验。结果表明,宏大七号等10个品种由于熟性、品质和产量等因素不符合品种审定标准建议退出试验,绿宝石(安)等4个品种可保留进行后续试验。     

广西矿区大气沉降与稻米重金累积的关系分析(英文)

笔者就大气沉降的概念、来源及其危害作了简要的概述,并对广西矿区大气沉降与稻米重金属累积的关系作简要分析,以期对广西矿区重金属沉降污染实施有效的风险预警,确保稻米安全生产提供参考。     

Effect of AIR on Inflammatory Reaction Infected by Brucella

In order to investigate the effect of AIR on inflammatory reaction infected by Brucellamelitensis (16M), the AIR domain of Tecpr1 gene of murine macrophages RAW264.7 were knocked down (I-A), overexpressed (O-A) and reversed (OA-IA). Using the chlorine fluorescein (DCFH-DA) as a probe, we detected the variation of ROS production and mitochondria distribution by confocal laser scanning microscopy. We observed the expression changes of NLRP3, ASC and Caspase-1 by qRT-PCR and the expression changes of IL-18,IL-1β and Caspase-1 in host cells by ELISA. The results showed that 16M could stimulate RAW264.7 cells to produce ROS by time-dependent pathway, and I-A group and O-A group showed more abnormal accumulation of mitochondrial. The results of qRT-PCR and ELISA suggested that it had effect on the expression levels of NLRP3, ASC,Caspase-1 and IL-18, IL-1β and Caspase-1 in cells of different groups. Those results indicated that with AIR gene deletion, the release amount of ROS changed, mitochondrial clustered abnormally, and AIR was closely related to the activation of inflammasomes and induction of inflammatory reactions.     

Cloning and Protein Structure Analysis of Hypoxia Inducible Factor-1α Gene in Yak

In this study,hypoxia inducible factor-1α(HIF-1α) gene was cloned from yak spleen by RT-PCR,and it was divided into two sections for amplification.The sequence and protein structure were analyzed using related bioinformatics tools.The length of the coding region was 2 472 bp,which encoded 823 amino acids.Its molecule mass was 92.13 ku,and PI was 5.09.It displayed as a hydrophilic protein,whose secondary structure was mainly composed of random coil and α-helix.It had 69 phosphorylation sites and 4 N-glycosylation sites.Phylogenetic tree displayed that Maiwa yak,Bos grunniens,Bos mutus and Bos taurus gathered into one cluster.Maiwa yak HIF-1α gene was successfully cloned in this study,it provided a viable reference for further study of genetic characteristics of HIF-1α.