‘三棱榄''橄榄果实香气成分分析

1 材料与方法选取广东优良鲜食橄榄品种‘三棱榄’,2001年12月6日采样,采用固相微萃取法(SPME)富集香气成分(鲜橄榄果肉于15℃下捣碎后取样1.0 g放入4 mL聚四氟乙烯硅橡胶垫密封螺口玻璃瓶中,插入100μm聚二甲基硅氧烷纤维头于室温25-30℃顶空取样2 h),用美国Finnigan TRACE GC-MS气相色谱-质谱联用仪进行分析。气相色谱柱为DB-1弹性毛细管柱30 m×0.25 mm,载气为He(99.99％),流速1.0mL/min。程序升温从40℃开始先保持10 min,后以2℃/min的升温速率升至150℃保持10 min。质谱条件:电子能量70 eV,离子源温度250℃,质量范围35-450 aum,不分流进样。2002年12月18日采样重复分析。     

Expression and role of CD134 and NF-κB in renal tissue of lupus nephritis

AIM:To investigate the role of CD134 (OX40) and NF-κB in the pathogenesis of lupus nephritis (LN).METHODS:Renal in situ CD134 and NF-κB expression were examined in 40 biopsy specimens from LN patients by immunohistochemistry and microwave-based immunohistochemistry, respectively. The relationship between expression of CD134 and NF-κB was analyzed.RESULTS:The expression of glomerular and tubular CD134 and NF-κB in LN were higher than that in normal control, especially in class Ⅳ LN, where there was intense staining of endothelial cell, distal tubules, and interstitial mononuclear cell. The CD134 expression of glomerular and tubular was closely related to NF-κB expression, respectively (r=0.5542, P＜0.05;r=0.6279, P＜0.05). CONCLUSIONS:The abnormal expression of costimulatory molecule CD134 was well evidenced in LN. Strong expression of renal in situ NF-κB was likely mediated by CD134 signal pathway, which may play an important role in the pathogenesis of LN.     

Effect of fibronectin on proliferation and collagen synthesis in cardiac fibroblasts from spontaneously hypertensive rats

AIM:To investigate the effects of fibronectin (FN) on the proliferation and collagen synthesis in cultured rat cardiac fibroblasts (CFb) derived from SHR (CFb_SHR) and WKY (CFb_WKY). METHODS:CFb derived from 12-week-old spontaneously hypertensive rat (SHR) and WKY was cultured by outgrowth of tissue block. Cell proliferation of CFb was measured by cell number counting and[³H]-TdR incorporation using 24-well plates pre-coated with 5 μg/cm² of FN. Collagen synthesis was determined by [³H]-proline incorporation. RESULTS:As compared with control, the cell number of fibroblasts derived from SHR and WKY were significantly increased to 163.75% and 170.42% respectively after 72 h incubation with FN in the presence of 0.4% FCS from a intial cell density of 1×10⁴ cells/mL. DNA synthesis of CFb was markedly promoted by FN. FN induced an increased in [³H]-proline incorporation in both CFb_SHR and CFb_WKY. CONCLUSION:FN is able to promote cell proliferation and collagen synthesis of CFb derived both from SHR and WKY.     

孔雀河流域绿洲生态支持系统调控模式研究

生态环境用水量的减少是孔雀河流域近 50年来现代绿洲环境退化的主要原因 ,维持生态与社会经济的合理用水比例是绿洲系统持续发展的前提。以此为基础 ,本文采用水资源承载力的方法探讨了绿洲生态支持子系统和社会经济子系统优化调控模式 ,最后就配置方案及发展模式的可行性进行了分析     

玉米疯顶病种子传播研究

连续3a对不同来源的玉米种子,利用显微镜镜检和试种观察植株发病显症的方法,检测其携带玉米疯顶病病菌状况。结果表明,病株病穗籽粒、病株健穗籽粒、病田健株籽粒都有较高的带菌率。因此,病菌随种子调运远距离传播是造成各地病害突发的主要原因。     

Effect of heat shock precondition on reperfusion arrhythmia in rats

AIM:To investigate the effect of the heat shock response on the reperfusion arrhythmias(RAs) and the possible mechanism involved. METHODS:Fifty-five Sprague Dawley rats were randomly divided into 2 groups: the heat shock group (group H,n=29) and the control group (group C,n=26). The rats in group H were preconditioned with heat shock 24 hours before, and that in group C were not. The hearts of 16 rats in group H and 16 in group C were exercised and mounted on a non-circulating Langendorff perfusion apparatus and perfused retrogradely with modified K-H buffer and mimic ischemia/reperfusion was applied. Additionally, conventional intracellular microelectrode techniques were used for recording such electrophysiological parameters as resting potential(RP), action potential amplitude(APA), over shot(OS), maximum depolarization velocity(Vmax) of the hearts of other 13 rats in group H and 10 in group C. RESULTS:①Prior heat stress significantly decreased reperfusion arrhythmia. ②The amount of CK release in the effluent in group H was much less than that in group C. ③Myocardial HSP70 content was elevated significantly in group H. ④Heat stress significantly increased myocardial anti-oxydases activity and decreased lipid peroxydative products. Additionally, heat stress significantly reduced the Vmax of action potential. It indicated that rapid Na⁺ channel of papillary muscles may be inhibited by heat shock. The degree of change of Vmax after ischemia in H group was significantly less than that in group C. And the time of reperfusoin with Tyrode's solution till the action potential appeared as large as that before perfusion with mimic ischemic solution is shorter in group H than in group C. CONCLUSION:Heat shock pretreatment markedly reduces ischemia/reperfusion-induced injury of heart and ventricular arrhythmias in rats and this effect may be associated with the inhibition of rapid Na⁺ channel of papillary muscles by heat shock and the increase in myocardial HSP70 and anti-oxydase activity.     

Effects of Taoren Honghua Jian on the hyperlipidemia without symptom

AIM: To examine the effect of traditional chinese medicine recipe, Taoren Honghua(semen persicae-flos carthami) decoction, on hyperlipidemia without symptom. METHODS: The plasma TC, TG, LDL, HDL, apolipoprotein(Apo) A, Apo Bof the patients with hyperlipidemia without symptom were measured using automatic analyzer (shimadiu CL-7200), the production of nitric oxide(NO) was detected by Greiss reaction, and SODactivity and MDAformation were examined using o-trihydroxy benzene and barbituric methotheds, respectively. RESULTS: After oral administration of Taoren Honghua decoction, the plasma levels of TC, TG, LDL, and MDAof the patients were markedly decreased, however, the plasma levels of ApoA, HDL, SODand NOwere significantly increased and almost no change was detected in the plasma level of Apo B. In control group, it was found that although the plasma level of TC, TG and LDL were decreased ( P<0.05 ) and ApoAas well as HDLwere increased, ApoB, SOD, MDA and NO production were all unchanged. CONCLUSION: The traditional chinese medicine recipe, Taoren Honghua decoction,has a significant therapeutic effect on patients with hyperlipidemia by removing blood stasis, promoting qi circulation and in turn reducing blood lipid level.     

优质耐贮中华猕猴桃新品种‘丰悦’、‘翠玉’

 ‘丰悦’、‘翠玉’是从野生猕猴桃资源中选育出的优质良种, 均果大质优, 平均单果质量均为90 g 左右, 最大127. 8 g、129 g。肉质细嫩多汁, 味甜, 香气浓, 可溶性固形物含量分别达13. 5%~ 15. 8%和14. 5%~ 17. 3%, 最高达19. 0%和19. 5% , 维生素C 含量分别达840~ 1 630 和930~ 1 430 mg/ kg。果实耐贮藏, ‘翠玉’20℃下可贮藏30 d 左右。在长沙‘丰悦’9月中下旬成熟; ‘翠玉’10月上旬成熟。     

应用电导仪测定番茄灰霉病菌对多菌灵抗药性的初步研究

1.0 mg/L和5.0 mg/L多菌灵盐酸盐溶液处理灰霉病菌多菌灵敏感菌株和抗性菌丝体,用电导仪测定溶液电导率的动态变化,结果表明:在敏感菌株存在时,多菌灵盐酸盐溶液2 h后的电导率显著降低,而抗性菌株存在的溶液中电导率则未见下降。     

稻瘿蚊综合防治新技术与示范

20世纪80年代后，稻瘿蚊发生危害明显回升，损失相当严重。以大田试验示范为主，系统研究了新耕作条件下稻瘿蚊综合防治，首次总结提出“喷药除草、压低虫源、抓住晚秧、做好测报、挑治本田”的综合防治新技术体系。室内外试验和大田示范表明，应用草甘膦杀死越冬寄主游草，可显著减少虫源基数；灭线磷(商品名益舒宝)、氯唑磷(商品名米乐尔)是防治稻瘿蚊的高效药剂，抗蚊青占品种高抗稻瘿蚊。此外，合理利用杀虫植物、性引诱剂和天敌等亦是防治稻瘿蚊的重要措施。在面积达12．46万hm^2的示范推广实践中，本田防治效果均达80％以上。