木霉菌在园林园艺植物上的应用及研究进展

木霉菌是目前生防菌中的研究热点,被普遍认为是最有可能代替多种化学杀菌剂的生防因子。随着木霉菌在农业生产中的广泛应用,木霉菌在园林园艺植物中的生物防治也受到广泛关注。现对木霉菌在园林园艺植物中生物防治和促生作用的研究进展进行概述,进一步阐述木霉作为生防菌的优势和不足,并对其在园林园艺植物上的应用进行前景展望。     

彭水县烟叶化学成分协调性不同年份间变异分析

为掌握重庆市彭水县烟叶协调性的时间变异特征,对2005—2014年上(B2F)、中(C3F)、下(X2F)部烟叶的协调性指标进行分析。结果表明:在彭水烟区烟叶钾氯比和两糖差变异系数较大,两糖比年度间变异最小,而其他化学成分含量的变异幅度在30%以内。从不同年份部位间变异来看,各化学成分及协调性指标均有逐渐稳定的趋势。从不同部位间对比来看,上部叶各化学成分协调性指标年度间变异总体上大于中、下部叶。     

Comparative Study on Carcass Traits and Meat Quality of Different Month Old of Xinjiang Brown Cattle Steers

In this research,12 heads of 28 to 30 months (low months age group) and 8 heads of 32 to 34 months (high months age group) Xinjiang Brown cattle steers which fed in the same mode of nutrition were chose for the slaughter test,in which the carcass traits,nutrient composition and some edible quality index were measured and comparative analyzed,a research which through comparative analysis the carcass traits and meat quality of nutritional components of different months of Xinjiang Brown cattle steers was carried out.The results indicated that the marbling,carcass grade,fat,meat color of high months age group were better than that of low months age group,the traits of the low months age group on fat color,moisture,protein,cooking loss and shear force value were better than that of high months age group,and there were significant difference on the traits of fat and meat color L^* between the two groups (P<0.05),while the differences of other traits between them were not significant (P>0.05);As high-grade beef,in addition to the traits of meat color and pH value of the chunk roll,tenderloin and striploin,each site of the meat traits on the fat,protein,moisture,cooking loss,shear force value indicators were all better than other parts,and showed the best of nutritional value and edible quality.28 to 34 months of Xinjiang Brown cattle steers could produce high-grade beef,the meat could be used as the raw materials of steak and boiled beef.     

水利施工的大体积混凝土抗裂技术

混凝土是水利施工中的主要的应用材料之一，为了保证水利施工中的安全与质量，必须保证混凝土的质量。文章全面分析了大体积混凝土产生裂缝的原因，并给出了相应的控制方法。     

我国社会公平现状及实现路径

贫富差距拉大,社会保障制度不健全,教育资源配置失衡,住房问题严重,是我国社会不公平的集中表现和原因所在。完善社会保障制度,合理配置教育资源,加大住房政策改革及实施力度是关键。     

7个红掌品种产生愈伤组织的影响因素

采用正交试验,探讨在不同光照条件下MS大量元素、6-BA、2,4-D、NAA对7个红掌品种愈伤诱导的影响.结果表明:散射光照对红掌愈伤诱导有促进作用;时红掌愈伤诱导影响排序为:Ms大量元素>NAA>6-BA>2,4-D,大量元素的影响效果最明显,6-BA和2,4-D的影响效果差异较小.     

奶牛TNMD基因的克隆与原核表达

从奶牛组织中克隆Tenomodulin(TNMD)基因的cDNA序列,采用双酶切后连接表达载体pGEX-4T-1,转入大肠杆菌BL21中进行诱导表达。结果表明,TNMD基因的序列全长为957 bp,通过双酶切构建的表达载体pGEX-TNMD在BL21大肠杆菌中成功表达了分子量为59.68 kDa的融合蛋白。     

(台中科技大学 应用中文系，台中 台湾404)

庄子其人，个性好自由不受拘束，思想超乎常人想像之外，笔调流畅，时间、空间及世人的看法所不能拘束，生死富贵也不能束缚他。《庄子》其书，可与《老子》同样尊贵，同样成为道家学术思想之主流，主导了魏晋玄学的哲学思路，影响了佛教中国禅的形成，也成为宋明理学的重要理论基础。而他的让心灵自由（心斋坐忘），更是道教上清斋醮最高修炼法门。庄子其人其书，对后世的影响，可以说是至深且广。但庄子其人，有宋人、魏人之争；《庄子》其书，则有真假及篇章多少之辩。今以出土文物及现存史料看来，庄子应为宋人，《庄子》书应为庄子所著，而内、外、杂三篇的形成，则当在魏晋南北朝时，系沿袭晋·郭象之归类而来。     

Evaluation on Tolerance of Montmorillonite as a Mycotoxin Adsorbent

In order to study the tolerance dose of Kunming mice to montmorillonite as a mycotoxin adsorbent in diets,the experiment selected 60 healthy male Kunming mice with initial average body weight of (17±0.12)g,randomly divided them into 5 treatments with two replicates per group and 6 mice per replicate.Five experimental diets were added to 0 (control group),0.5%,1%,2% and 5% montmorillonite,respectively.After 30 days,the growth performance,nutrients apparent digestibility,serum biochemical indices and internal organization were tested to determine the tolerance dose of montmorillonite for Kunming mice.The results showed that the ADFI was significantly increased in 0.5% group (P< 0.05),while there were no significant differences in ADFI and ADG between 1% group and control group (P> 0.05),then the ADFI was significantly decreased with the increasing of montmorillonite supplemental level (P< 0.05).The ADG in 2% group had no significant difference when compared with the control group (P> 0.05),while significantly lower than that in 0.5% and 1% groups (P< 0.05).The apparent digestibility of DM,CP and EE showed no significant effect at any level of montmorillonite (P> 0.05).Serum ALT,AST activities and MDA content were gradually increased with the montmorillonite supplemental level increasing,and that in 1%,2% and 5% groups were significantly higher than that in control group (P< 0.05),but there were no significant differences between 0.5% group and control group (P> 0.05).Tissue sections showed that mice livers were damaged in varying degrees when the montmorillonite supplemental level was 5%.In conclusion,based on a comprehensive consideration of all indexes,it could be seen that the tolerance dose of the tested montmorillonite for Kunming mice was between 0.5% and 2% when mycotoxin contents in diets were in security range.     

2株可培养细菌氮代谢特性的研究

研究旨在分析土壤中可培养细菌菌株的氮代谢特征,并进一步探讨微生物在土壤氮素转化中的可能作用机制。以2株分离自苹果园土壤的细菌菌株SY5-4和SY11-10为试材,采用传统培养方法结合分子检测技术,分别测定菌株生长特性及其氮素转化能力。研究结果表明,异养条件下,菌株SY5-4和SY11-10的世代时间分别为243.5 min和202.7 min。菌株生长过程中,培养液中铵态氮浓度始终维持在较高水平,铵态氮、亚硝态氮和硝态氮浓度均表现出先升后降的趋势。硝化（amoA和hao）和反硝化（nosZ、norB、nirK和nap）基因检测结果表明,菌株SY11-10具有多种氮素转化潜能。综上,供试菌株培养过程中,培养液中氮素发生变化,并在菌体中检测到不同氮转化基因,表明菌株参与多种氮代谢途径。