杨树露天嫩枝扦插育苗技术研究

杨树嫩枝扦插扩繁快,效果好,对一些珍贵杨树扩繁可行。扦插分4个阶段即自养阶段、生根阶段、缓苗阶段、生长阶段。每年最佳扦插时间为6月上旬至8月上旬。插穗带2~3片叶,叶面积100 cm2最佳。     

中华按蚊几丁质酶基因家族的全基因组鉴定与分析

本研究基于生物信息方法对中华按蚊的几丁质酶基因家族进行全基因组鉴定与分析.利用BLAST、HMM及Softberry网站中的基因预测程序进行序列鉴定、检验及补充,筛选出23个中华按蚊几丁质酶基因及类似基因,并进行系统发生分析,将中华按蚊几丁质酶基因分为八大家族,其外显子数为2～13个,跨度较大;基因分布分析结果表明As...     

Aortic expression of HSP22, TNF-α and eNOS in rats with hyperlipidemia and effects of atorvastatin

AIM:To establish a rat hyperlipidemia model for studying the aortic expression of heat shock protein 22 (HSP22), tumor necrosis factor alpha (TNF-α) and endothelial nitric oxide synthase (eNOS) and the effect of atorvastatin intervention. METHODS:Hyperlipidemia model was established in SD rats. Afterwards, the rats were divided into normal control group, high fat group and high fat+atorvastatin intervention group. The expression of HSP22 and TNF-α in the rat aortas was detected by immunohistochemical assay and the expression of eNOS was assessed by Western blotting. RESULTS:No detectable expression of HSP22 and TNF-α in the normal control group was observed. However, the expression of HSP22 and TNF-α was positive in the high fat group and the atorvastatin intervention group. The mean densities of HSP22 and TNF-α positive particles were significant lower in the atorvastatin intervention group as compared with high fat group (both P＜0.05). The expression of eNOS protein in the high fat group and atorvastatin intervention group was significantly lower than that in normal control group (P<0.01). However, no marked difference of eNOS protein expression between high fat group and atorvastatins intervention group was observed. CONCLUSION: The expression of HSP22 and TNF-α in the rat aortas is increased in the hyperlipidemia rat model. This effect can be restored by atorvastatin treatment. The expression of eNOS in the rat aortas is decreased in the hyperlipidemia rat model, but this tendency could not be attenuated by atorvastatin.     

Expression of TMEM16A as a calcium-activated chloride channel in Fischer rat thyroid follicular epithelial cells and its electrophysiologic pro-perties

AIM：To investigate the expression of transmembrane protein 16A(TMEM16A) in Fischer rat thyroid follicular epithelial (FRT) cells and its electrophysiologic properties. METHODS：The eukaryotic expression vector of pUB6/V5-TMEM16A was constructed and transfected into FRT cells by liposome-mediated transfection. In order to obtain the high efficiency of gene transfection and expression, the quantity and ratio of lipid/DNA complexes were optimized. The FRT cells stably expressing TMEM16A were gained by the selection with blasticidin and confirmed by the techniques of RT-PCR and immunofluorescence. The expression and location of TMEM16A in the FRT cells were observed under an inverted fluorescence microscope. TMEM16A protein was associated with calcium-dependent chloride current, as measured with halide-sensitive fluorescent protein and patch-clamp technique. RESULTS：The results of double digestion and sequencing indicated that TMEM16A was cloned into pUB6/V5. The results of RT-PCR and immunofluorescence confirmed that TMEM16A was expressed in the FRT cells after transfection with TMEM16A. The classical calcium-activated chloride channel currents were recorded in the FRT cells stably expressing TMEM16A by the technique of patch-clamp and halide-sensitive fluorescent protein YFP-H148Q/I152L. CONCLUSION：The protein expression of TMEM16A in the FRT cells was observed. TMEM16A is the molecular identity of calcium-activated chloride channels.     

Major histocompatibility complex and mate choice in the polygynous primate: the Sichuan snub‐nosed monkey (Rhinopithecus roxellana)

The highly polymorphic genes within the major histocompatibility complex (MHC) not only play a major role in immunity resistance, but also seem to provide hints for mate choice in some animal populations. In the present study we investigated MHC‐related mate choice in a small natural population (group size 40–55 individuals) of a polygynous primate, the Sichuan snub‐nosed monkey (Rhinopithecus roxellana). We found that there was no evidence either for MHC‐disassortative mating, or for females to mate with males based on MHC heterozygosity or specific alleles. Nevertheless, of the 11 alleles identified, we found that the frequencies of 2 alleles, Rhro‐DRB2 (P < 0.01) and Rhro‐DRB5 (P < 0.05) were higher in offspring than in their parents. These findings suggest that MHC‐DRB in this population of R. roxellana is unlikely to be associated with mating preferences. Limited female opportunities for mate choice are likely due, in part, to the harem breeding structure present in R. roxellana, and the relatively small number of resident adult males in our study band (N = 4–6). In addition, we suggest that differences in the frequency of particular alleles across generations may be linked to parasite resistance in a fluctuating environment; however, confirmation of this finding requires further study.     

川东北丘陵地区紫山药高产栽培技术研究

紫山药为特色蔬菜新品种,营养价值高。南充作为川东北农业第一大市,首次引进紫山药栽培。我们设计正交试验对其株行距、施肥方案进行研究,形成适合川东北地区气候地理特点的紫山药高产栽培技术,每667 m2产量可达1 700 kg以上,推广前景广阔。     

四川省科技成果现状分析与对策研究

为了提高四川省的科技创新能力,有针对性地开展科学研究,通过对四川省2001-2009年科技获奖成果统计分析,提出了加快该省科技创新体系建设的若干建议。     

一个紫云英F;重组自交系群体的农艺性状与养分吸收评价北大核心CSCD

利用以闽紫7号紫云英为母本、信阳种紫云英为父本获得的F;代群体,评价了该群体18个株系的农艺性状和养分吸收状况,为筛选优异紫云英株系做参考。结果表明,在盆栽条件下,不同株系紫云英在盛花期表现出一定的性状变异。相关性分析表明,鲜重、干重与氮、磷、钾积累量相关性高,对植株的养分吸收影响较大;株高、分枝数、真叶数是影响植株地上部鲜重、干重的重要因素。主成分分析表明,地上部鲜重、干重以及地上部氮、磷、钾积累量是反映紫云英株系性状优劣的主要指标。聚类分析结果显示,当距离系数d=25时,18份紫云英材料可分为3类,其中Ⅰ类群综合性状最优,包括m83xzh-1-4-4、m83xzh-1-4-6、m83xzh-1-4-8和m83xzh-1-4-13共4个株系。综合考虑紫云英生长性状、鲜草产量及植株养分积累量等因素,m83xzh-1-4-4的地上部磷、钾积累量和地下部氮、钾积累量最高,每盆分别高达11.00 mg,145.18 mg,22.15 mg和15.27 mg,同时该株系具有较高的鲜草产量,每盆高达44.63 g。本研究可为后续按目标性状选育紫云英新品种提供参考。