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41.
In order to establish an indirect ELISA method for detection of equine herpesviruses type 4 (EHV4) antibody, the glycoprotein G (gG) protein with specific epitope worked as a detection antigen. After optimizing conditions, the indirect ELISA method was developed successfully,specificity and repeatability tests were determined. The result was that the EHV4 gG only reacted with antibodies against EHV4;but not with antibodies against EHV1; both the intro-batch and inter-batch variation coefficiencies were lower than 10%.Concordance of the indirect ELISA relative to commercial EHV1/4 antibody kit was above 90%.The results indicated that the indirect ELISA method could be used for the detection and epidemiological surveys of EHV4 infection. 相似文献
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Fat is an essential element for animal.The paper reviewed recent research progress on fat nutrition in monogastric animal at home and abroad, combining with the fat digestion and absorbing form in animal body, and summarized biological function and application of fat in production, so as to provide theoretical basis for better utilization of fat in animal production and exploration its mechanism of action. 相似文献
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湘紫薯174 是以浙紫薯1 号为母本、浙紫薯3 号为父本杂交选育而成的食用型紫心甘薯新品种,薯块纺锤形,薯皮
紫红色,薯肉紫色,结薯较集中整齐,单株结薯4~5 个,大中薯率82.7% 以上,熟食味好,抗黑斑病,中抗根腐病、茎线
虫病和薯瘟病;每667 m2 鲜薯产量1 913~2 359 kg,薯干产量549~736 kg;花青素含量为714.5 mg · kg-1(FW)。适宜在湖
南、湖北、江西、江苏、浙江等地春夏薯区种植。 相似文献
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AIM: To observe the treatment effect and its immune regulation of human amnion epithelial cells (hAECs) on Alzheimer's disease (AD)-like pathology rat model. METHODS: The hAECs were isolated from amnion with trypsin digestion, and the phenotype of hAECs was analyzed by flow cytometry. SD rats (n=48) were randomly divided into sham control group, model group, medium group and hAECs group. AD-like pathology rat model was induced by bilateral intraventricular injection of lipopolysaccharide (LPS). hAECs (5×105) were injected into the hippocampus of the AD-like pathology rats. At 2 weeks after transplantation, the animals were tested by Morris water maze to observe the function of learning and memory. The pathological change of the brain was observed by HE staining. The expression of amyloid β-protein 42(Aβ42) and Tau protein and the level of acetylcholine (ACh) in the injury brain were determined by immunohistochemistry. The survival and differentiation of hAECs in the hippocampus were measured by immunofluorescent technique. The percentages of lymphocyte subsets in the peripheral blood mononuclear cells were analyzed by flow cytometry. The contents of serum cytokines were detected by cytometric bead array. RESULTS: Compared with model group and medium group, hAECs group showed shortened escape latency (P<0.01), increased frequency of going through the platform (P<0.05), reduced loss of hippocampal neurons, decreased expression of Tau protein and Aβ42 in the hippocampus (P<0.05), increased ACh level in the hippocampus (P<0.05), decreased percentages of Th1 and Th17 subsets, increased percentages of Th2 and Treg cells (P<0.05), decreased concentrations of IFN-γ and IL-2 in the serum, and increased concentration of IL-4(P<0.05). CONCLUSION: hAECs improve the cognitive learning and memory function and alleviate pathologic damage of hippocampus through immune regulation in AD-like pathology rats. 相似文献
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试验通过研究不同生长速度阿勒泰羊主要血液生化指标含量和尾脂中瘦素(Leptin)基因的表达水平,探讨阿勒泰羊生长速度与脂肪沉积之间的关系。选取300只出生时间相近的阿勒泰羊公羔,正常饲喂至6月龄,记录日增重,取平均日增重排前10%和后10%的公羔各30只,分为生长快速组(HBW)和生长慢速组(LBW),从生长快速组随机选12只分为抑制生长组(饲喂低能量饲粮以抑制其生长,HBW-75%,n=6)和生长快速对照组(饲喂标准饲粮,HBW-100%,n=6),生长慢速组随机选12只分为促进生长组(饲喂高能量饲粮以促进生长,LBW-125%,n=6)和生长慢速对照组(饲喂标准饲粮,LBW-100%,n=6);预饲期7 d,正饲期30 d,试验结束后称重并采集血液,屠宰后采集尾部脂肪。检测血液TG、CK、TSH、T3、T4、S.S、GH、INS、PG、Leptin含量或活性及尾部脂肪Leptin mRNA和蛋白表达量,并观察尾部脂肪细胞形态学变化。结果显示,HBW-100%和LBW-100%组初体重与末体重均差异显著(P<0.05),HBW-75%和HBW-100%组初体重与末体重均无显著差异(P>0.05);LBW-100%和LBW-125%组初体重差异显著(P<0.05),末体重无显著差异(P>0.05)。LBW-100%组的T4、S.S、Leptin、TSH、T3含量显著低于HBW-100%组(P<0.05);LBW-125%组T3含量显著高于LBW-100%组(P<0.05);HBW-75%组GH、PG和Leptin含量显著低于HBW-100%组(P<0.05)。LBW-125%组尾部脂肪细胞面积大于LBW-100%组,HBW-75%组小于HBW-100%组(P<0.05)。Leptin mRNA和蛋白表达量检测结果表明,HBW-100%组的Leptin mRNA表达量与LBW-100%组无显著差异(P>0.05),Leptin蛋白表达量极显著高于LBW-100%组(P<0.01)。LBW-125%组Leptin mRNA和蛋白的表达量均显著高于LBW-100%组(P<0.05)。HBW-75%组Leptin mRNA的表达量极显著低于HBW-100%组(P<0.01),Leptin蛋白表达量显著低于HBW-100%组(P<0.05)。综上所述,生长快速组与生长慢速组中,Leptin的表达量与体重和体脂有一定关联;在生长慢速组促进生长后体重和体脂显著增加,Leptin的表达量呈上升趋势;在生长快速组抑制生长后体重和体脂减少,Leptin的表达呈下降趋势。改变生长速度对Leptin的表达量会产生影响,从而改变机体的脂肪沉积状况。 相似文献