Effects of active immunization against myostatin on carcass quality and expression of the myostatin gene in pigs

The study was conducted to investigate the effects of active immunization against myostatin on the titer of myostatin antibody, carcass evaluation, activity of creatine kinase and the expression of the myostatin gene in pigs. Eighteen pigs were allotted into three groups (six pigs per group), and pigs in treatment 1, 2 and 3 were immunized with physiological saline, 1 mg or 4 mg myostatin per pig, respectively. Six pigs were killed by electrical stunning followed by exsanguination at BW of 100 kg. The results indicated that the titer of myostatin antibody was increased in treated groups compared to the control group on day 42 ( P  < 0.01) and d 84 ( P  < 0.01). The carcass lean percentage was significantly increased in the treatment groups compared to the control group ( P  < 0.01), and intramuscular fat was significantly decreased in the 4 mg group compared to the control group ( P  < 0.05). The muscle creatine kinase activity of pigs treated with 1 mg and 4 mg myostatin was lower than the control group. The immunization of myostatin signofocantly decreased the myostatin gene expression levels in muscle. It was concluded that optimal active immunization against myostatin could increase the content of myostatin antibody, suppress the activity of creatine kinase and the expression of myostatin gene, and therefore improve the carcass lean percentage for pigs.     

三叶鬼针草水提液对蒲公英种子萌发和幼苗生长的化感作用

以蒲公英试材,采用培养皿法和水培法,研究了入侵植物三叶鬼针草(Bidens pilosa)水提液对蒲公英(Taraxacum mongolicum)种子萌发和幼苗生长的化感作用。结果表明:不同浓度的三叶鬼针草水提液处理均降低了蒲公英种子的发芽势、发芽率、发芽指数和活力指数,抑制了蒲公英幼苗的根长、苗高和叶宽生长,增加了丙二醛含量及相对电导率。说明入侵植物三叶鬼针草水提液对蒲公英有较强化感作用,使蒲公英种子萌发及幼苗生长受到抑制。     

Downregulation of DEK induces cell apoptosis via inhibition of NF-κB signaling pathway in gastric carcinoma SGC-7901 cells

AIM: To investigate the effect of DEK downregulation on the apoptosis of gastric carcinoma SGC-7901 cells, and to explore its associations with NF-κB signaling pathway and apoptosis related proteins. METHODS: SGC-7901 cells with different treatments were divided into 3 groups including untreated group, control siRNA group and DEK siRNA group. The expression of DEK at mRNA and protein levels in the SGC-7901 cells was detected by real-time PCR and Western blot. The cell apoptosis was examined by flow cytometry. Furthermore, the activities of caspase-3 and caspase-9 in the SGC-7901 cells were investigated by Caspase-Glo^®-3/9 kit. Finally, the expression of key regulatory protein p65 of NF-κB signaling pathway and apoptosis-related proteins Bcl-2 and Bax in the SGC-7901 cells was investigated by Western blot. RESULTS: Compared with untreated group and control siRNA group, the expression of DEK at mRNA and protein levels was significantly downregulated in DEK siRNA group (P<0.05). In addition, the ratios of early phase apoptosis and total apoptosis in DEK siRNA group were markedly higher than those in untreated group and control siRNA group (P<0.05). Most notably, the decrease in p65 and Bcl-2 proteins, increase in Bax protein and the increases of caspase-3 and caspase-9 activities were observed in DEK siRNA group. CONCLUSION: Downregulation of DEK mediates cell apoptosis of gastric carcinoma may be tightly associated with NF-κB signaling pathway.     

干旱处理对华北绣线菊和毛果绣线菊光合作用及相关蛋白表达的影响

以抗旱力弱的毛果绣线菊与抗旱力强的华北绣线菊为试验材料,采用不同程度干旱处理,在测定其光合作用变化的基础上,采用蛋白质组学技术研究两种绣线菊光合作用相关蛋白表达的变化。结果表明:干旱胁迫两种绣线菊光合能力降低,光补偿点和光饱和点降低,说明利用弱光能力提高,华北绣线菊适应弱光环境的能力强于毛果绣线菊。不同水平干旱处理分别鉴定出涉及能量代谢与运输的相关蛋白20种。干旱胁迫下毛果绣线菊中卡尔文循环途径相关酶、光系统Ⅱ中多数放氧复合蛋白和捕光色素复合蛋白、参与光合磷酸化的酶发生显著上调和下调表达;华北绣线菊中参与卡尔文循环代谢相关酶、PSⅠ中反应中心蛋白和参与光合电子传递的蛋白、PSⅡ中捕光色素复合蛋白、参与光合磷酸化与氧化磷酸化的蛋白发生明显的上调和下调表达。干旱胁迫使抗旱力不同的毛果绣线菊和华北绣线菊光合作用相关蛋白均受到影响,毛果绣线菊中多是能量代谢相关蛋白发生显著变化,华北绣线菊中多是能量运输相关蛋白发生显著变化。     

基于因子分析的核桃品质综合评价研究

为从众多核桃品质的评价指标中找到主导的影响因子,达到用最少的指标获取最多核桃品质信息的目的,试验以赫章8个单株核桃为试材,通过对表征核桃物理化学特性的12项指标进行测定,利用因子分析,对众多指标进行降维处理,找出少数几个影响核桃品质的主导因子。结果表明:12项原始指标归属为3个相互独立的公因子,累计方差贡献率达到85.960%,其中,出仁率和壳厚度是重要的核桃品质的物理指标,蛋白质含量是重要的化学品质评价指标。核桃的理化品质与亲缘关系之间有一定的联系,利用因子分析结果进行聚类分析发现,1、5、6号核桃理化品质相似,可能具有较近的亲缘关系。4、7、8号单株单独成为3类,说明可能与其它单株的亲缘关系较远。研究结果可在一定程度为核桃资源的品质鉴定、优株鉴选、分类及亲缘关系的确定等提供理论依据。     

三十七个山东牡丹品种DNA指纹图谱构建与EST-SSR标记遗传多样性分析

以中国常熟尚湖牡丹园引栽的37个山东牡丹品种为试材,采用筛选出的10对多态性高、稳定性好且在染色体上分布均匀的EST-SSR标记引物,研究山东牡丹37个品种DNA指纹图谱和遗传多样性。结果表明:10对EST-SSR标记引物在37份材料中共扩增出了共计58个多态性基因型,平均每对引物5.8个,变幅2~13种;10对引物的多态性频率(FP)平均值为0.60;筛选出的4对核心引物可以将37个牡丹品种完全区分,以遗传相似系数0.69为阈值可将供试37个试牡丹品种分成5类。EST-SSR标记在供试牡丹品种间多态性差异较大,适合用于牡丹的DNA指纹图谱的构建。     

种子发芽纸测试报告

发芽纸是种子发芽试验必需的材料。国内首次研制成的专用种子发芽纸具有吸水快、持水性好、韧度大、厚度适中等特点。该纸无毒性、幼根不易穿透,且成本低。它既可衬垫于发芽容器中,更宜作发芽纸卷。试用结果表明,它能广泛适用于农作物、蔬菜、牧草、瓜、豆类等各种种子的发芽试验。在植物种子科学研究和生产实际中,经常需要进行种子发芽试验,一般的种子发芽试验常采用纸张作为发芽床。为使种子正常发芽和便于检查发芽结果,要求发芽纸有良好的理化特性。对此,国际种子检验协会对种子发芽纸的质地、灰分、韧度和吸水性能等有明确的规定指标,如要求纸张有足够强度,在发芽试验操作中不致撕破;质地要通透多孔,但幼根不能穿入纸中。国外已有不少国家都生产有专用的种子发芽纸,而国内至今还没有一种专门供种子发芽用的纸张,长期以来使用的是普通滤纸、卫生纸、乃至黄草纸等代用品。这些纸张在协调种子发芽所需的水气条件方面存有不少缺陷,其质地、强度或吸水性等都达不到要求。为了填补国内这一空白,浙江农业大学种子科学中心和浙江民丰造纸厂造纸研究所自1987年开始进行种子发芽纸的研制。经过多次反复研制和测试,已研制成功一种种子发芽纸。它厚度适中,既可平垫于玻皿之中,又可用作发芽纸卷,且成本低于目前代用的普通滤纸。经数家种子部门试用,反应良好。此将发芽纸研制过程中生物学适用性测试的部分内容报告如下。     

Measurement and Analysis of Industrial Productivity of Chongqing

Productivity is the most basic index to measure efficacy of a production system in industrial economy. The measurement of productivity is the first step of its management. With the huge push of Reform and Opening,industrial economy of Chongqing is keeping a steady growth.The authors use production function to measure the industrial productivity of Chongqing from 1979 to 1999,and analyze its developing trend.     

Test Bench of Air Braking System for Vehicles

The stability of the brake system is a key factor for the vehicle safety.It is important for the air braking system's stability to shorten the time lag between the front brake and the rear brake,the reasonable disposal of the air pipeline and the optimization of the braking system design.A test bench is developed to test the performance of the air braking system,which is mainly made of pressure sensors,signal amplifiers,data acquisition system,computer,pressing air resource and so on.Based on this test bench,the reaction time of the system while braking and abandoning brake can be measured.Using this test bench,the performances of the air braking system of a certain commercial vehicle is detected.Compared with the actual road test,it can save the cost and the time for experiment largely.