Replacing fish oil and astaxanthin by microalgal sources produced different metabolic responses in juvenile rainbow trout fed 2 types of practical diets

Dietary fish oil supplementation provides n-3 long-chained polyunsaturated fatty acids for supporting fish growth and metabolism and enriching fillet with eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; c22:6n-3). Two experiments were performed as a 3 × 2 factorial arrangement of dietary treatments for 16 wk to determine effects and mechanisms of replacing 0%, 50%, and 100% fish oil with DHA-rich microalgae in combination with synthetic vs. microalgal source of astaxanthin in plant protein meal (PM)- or fishmeal (FM)- based diets for juvenile rainbow trout (Oncorhynchus mykiss). Fish (22 ± 0.26 g) were stocked at 17/tank and 3 tanks/diet. The 100% fish oil replacement impaired (P < 0.0001) growth performance, dietary protein and energy utilization, body indices, and tissue accumulation of DHA and EPA in both diet series. The impairments were associated (P < 0.05) with upregulation of hepatic gene expression related to growth (ghr1and igf1) and biosynthesis of DHA and EPA (fads6 and evol5) that was more dramatic in the FM than PM diet-fed fish, and more pronounced on tissue EPA than DHA concentrations. The source of astaxanthin exerted interaction effects with the fish oil replacement on several measures including muscle total cholesterol concentrations. In conclusion, replacing fish oil by the DHA-rich microalgae produced more negative metabolic responses than the substitution of synthetic astaxanthin by the microalgal source in juvenile rainbow trout fed 2 types of practical diets.     

Extrapituitary and Pituitary Pathological Findings in Horses with Pituitary Pars Intermedia Dysfunction: A Retrospective Study

The objective of this study was to describe the histopathologic changes observed in extrapituitary organs as well as the pituitary glands of horses with pituitary pars intermedia dysfunction (PPID). Adrenal gland, thyroid gland, liver, lung, kidney, heart, and pituitary gland from 32 horses with clinical and histologic evidence of PPID and 20 control horses were reviewed histologically. Ten of the control horses were aged animals (≥15 years), allowing those changes attributed to age to be identified. In addition to previously reported changes in adrenal gland and liver, an association was established between PPID and several extrapituitary histopathologic changes, namely bronchiolitis, proliferative glomerulopathy, and myocardial lipofuscinosis and fibrosis. The potential biologic significance of these changes is discussed and, although the retrospective design of the current study precludes establishment of causal relationships between the observed extrapituitary changes and PPID, these findings suggest that further investigations are warranted.     

Effect of post-insemination progesterone supplementation on pregnancy rate in dairy cows

Progesterone plays an important role in maintenance of pregnancy. It is hypothesized that insufficient progesterone early in pregnancy may result in embryonic loss, and that supplemental progesterone would decrease pregnancy loss in dairy cows. In Experiment 1, 84 cows and 16 heifers from a single dairy operation were selected randomly. Within each age category, controlled internal drug release (CIDR) devices were inserted into the vagina of every other female on Day 4 post-insemination and removed on Day 18 post-insemination. Transrectal ultrasonography was performed to determine pregnancy at 4 time periods [days 30 to 37 (week 5), days 44 to 51 (week 7), days 58 to 65 (week 9), and days 86 to 93 (week 13)]. Progesterone supplementation had no effect on pregnancy rate. In Experiment 2, there were no differences in progesterone concentrations between cows that did and did not receive a CIDR. Further, cows receiving CIDR devices did not have an increase in circulating progesterone concentrations 30 min or 1 h after CIDR insertion. It appears that progesterone supplementation does not increase circulating levels of progesterone in the early pregnant lactating dairy cow. Alterative methods to influence progesterone concentrations and/or early embryonic loss need to be investigated.     

Meniscal Mineralization in Domestic Cats

Objective: To (1) determine prevalence of radiographically detectable meniscal mineralization in domestic cats and (2) to evaluate the association between meniscal mineralization and degenerative joint disease (DJD). Study Design: Prospective study. Animals: Client‐owned cats (n=100) and 30 feline cadavers. Methods: Randomly selected client‐owned cats were used to determine the prevalence of meniscal mineralization. Stifles from feline cadavers were used to evaluate the relationship between meniscal mineralization (using high‐resolution X‐ray), radiographic DJD, and cartilage damage. Menisci were evaluated histologically. Results: Forty‐six percent of the client‐owned cats had meniscal mineralization detected in 1 or both stifles. Pain scores were not significantly different between stifles with meniscal mineralization and those with no radiographic pathology (P=.38). Thirty‐four of 57 cadaver stifles had meniscal mineralization, which was always located in the cranial horn of the medial meniscus. Percentage mineralization of the menisci was significantly correlated with the cartilage damage score of the medial femoral (r²=0.6; P<.0001) and tibial (r²=0.5; P<.0001) condyles as well as with the total joint cartilage damage (r²=0.36; P<.0001) score and DJD score (r²=0.8; P<.0001). Conclusion: Meniscal mineralization is a common condition in domestic cats and seems to indicate medial compartment DJD. Clinical Relevance: Clinical significance of meniscal mineralization is uncertain. Further work is needed to determine if the meniscal mineralization is a cause, or a consequence of joint degeneration.     

Signal detection theory clarifies the concept of perceptual range and its relevance to landscape connectivity

Understanding functional connectivity is critical for several issues in ecology and conservation. When animals actively search for habitat across landscapes, their perceptual range of habitats can profoundly influence connectivity. Nonetheless, conceptual development and estimation of perceptual ranges and their influence on connectivity have been limited. Signal detection theory (SDT) has a long tradition in several disciplines to address the problem of detecting stimuli in noisy and uncertain environments. SDT is particularly useful for understanding perceptual ranges because it acknowledges uncertainty in the detection process and distinguishes between two key parameters that have previously been confounded when interpreting the perceptual range of animals: signal detectability and response bias of individuals. Here we extend SDT to the concept of perceptual range, provide approaches for estimating patch detectability and response bias, and apply this framework to interpreting the perceptual range of cactus bugs (Chelinidea vittiger). We find that signal detectability of habitat by C. vittiger, and thus their perceptual range, is between 1 and 2 m, based on generalized linear models aimed at estimating signal detection parameters. SDT provides new insights into perceptual ranges and functional connectivity, which may help understand intra and interspecific variation in animal responses to modified landscapes.     

The effect of rootstock shoot growth on the level of bud-grafting success in apple

Poor stands of ‘Queen Cox’ apple bud-grafted in summer onto M.9 rootstock were shown to be due to the scion bud not growing, or growing too late to produce a worthwhile ‘maiden’ tree, despite virtually all scion graft-chips forming unions with the rootstock. This problem was caused by raising the budding height to 30 cm above ground, as a method of increasing the height of lateral branches. The key element in higher budding which depressed subsequent scion bud growth was the complete removal of all rootstock shoots to a height of 35 cm to give a traditional ‘clean leg’ during the budding year. Removing rootstock shoots only at the budding position gave good stands of high quality trees. This appears to be a general effect, with the benefits of retaining rootstock shoots applying also to Malus tschonoskii when budded at 30 cm on M.9 rootstock. For both ‘Queen Cox’ and M. tschonoskii, failure of scion buds to grow vigorously often resulted in the development of ‘papery bark canker’ on the M.9 rootstock. The main benefit of root- stock shoots retained in the budding year was to ensure that scion buds grew early in the following ‘maiden’ year, whereas allowing these and new rootstock shoots to remain for the first two months of ‘maiden’ growth reduced the number of secondary scion buds which developed, and raised the height of laterals by delaying their emergence.     

Economic development and natural amenity: An econometric analysis of urban green spaces in China

Rapid urbanization and spontaneous economic development has brought about profound changes in urban landscapes throughout the world. The task of managing transforming urban landscapes, particularly urban green spaces, so as to provide sufficient natural amenity for increasing urban populations, is one of the critical challenges facing policy makers. However, little empirical evidence exists about the evolving path of urban green spaces along with economic development and urbanization. This study attempts to fill in this knowledge gap through an econometric analysis of panel data across 285 Chinese cities during a period of rapid urbanization and economic growth (2001–2010). The results point to the existence of an N-shaped environmental Kuznets curve (EKC) for an important aspect of environmental quality: urban green spaces. Urban green space coverage increases at the initial stage of economic development, and then it starts to decrease as GDP per capita exceeds RMB50,855 and then increases again at a high GDP per capita level (RMB107,558). Large elasticity (>1) is expected as GDP per capita grows to a higher level (beyond RMB128,095). By the end of 2010, 30% of Chinese cities are still located on the downward-sloping path and only four cities have attained elasticities greater than one. The findings present a challenging and pressing call for policy makers to effectively manage the tradeoffs between continuous economic development and better natural amenities.