Polyamines enhance chilling tolerance of cucumber (Cucumis sativus L.) through modulating antioxidative system

Cucumber chilling-resistant cultivar Changchun mici and -sensitive cultivar Beijing jietou were used in this study to investigate the effects of exogenous PAs on protection against chilling injury as well as on changes of physiological features, and the fluctuation of free PAs content in the leaves under chilling stress. Upon chilling treatment, free spermidine (Spd), spermine (Spm) and putrescine (Put) were remarkably induced in the leaves of cv. Changchun mici 1 day after treatment. The induction of Put declined thereafter, whereas Spd and Spm levels increased steadily. In the leaves of cv. Beijing jietou, Put content was increased only at 1 day after chilling while Spd content decreased significantly upon chilling treatment. Chilling reduced soluble protein content, and decreased the activities of antioxidant enzymes, including superoxidase dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) only in Beijing jietou. However, these changes could be renovated by exogenous application of Put and Spd. It was also found that pretreatment with Put and Spd diminished the increased electrolyte leakage and malondialdehyde (MDA) content caused by chilling in the leaves of both cultivars. Pretreatment of methyglyoxal-bis-(guanylhydrazone) (MGBG), the PAs biosynthetic inhibitor cancelled the effects of PAs in most of the treatments. Moreover, histochemical staining and quantitative measurements showed that exogenous application of Put and Spd eliminated but MGBG exaggerated the hydrogen peroxide (H₂O₂) accumulation caused by chilling stress, especially in leaves of Beijing jietou. Interestingly, Changchun mici was found to contain higher endogenous free PAs contents compared to Beijing jietou. While no significant difference of SOD, POD and CAT activities was found between non-chilling Changchun mici and Beijing jietou seedlings, the former exhibited higher APX activity than the latter. These results suggest that PAs play important roles in the tolerance of cucumber against chilling stress, which is most likely achieved by acting as oxidative machinery against chilling injury.     

Role of nestin in the maintenance of the structure of podocytes

AIM:To investigate the expression of nestin, a kind of cytoskeletal protein in cultured murine podocytes and the role of nestin in the maintenance of the podocyte structure.METHODS:The immortalized murine podocytes were cultured. The expression of nestin was determined by immunofluorescence. In differentiated podocytes, the expression of nestin was knock-down by RNAi. The effect of nestin knock-down was examined by Western blotting and immunofluorescence. The projections longer than maximal length of the cell body in which the cells transfected with siRNA and control vector that contained nonhomologous oligo were counted, respectively. RESULTS:Nestin siRNA markedly reduced or abolished nestin expression. In cells transfected with nestin siRNA, the percentage of cells with processes was significantly lower than that in cells transfected with control vector (77.0%±6.3% vs 16.0%±4.6%, n=3, P<0.01). CONCLUSION:Nestin may play an important role in maintaining normal function of podocytes.     

Effects of salvianolic acid B on neurons during oxygen/glucose deprivation and reintroduction

AIM: To observe the damage induced in the primary cultured rat cortical neurons by oxygen/glucose deprivation and reintroduction, and to investigate the neuroprotective mechanism of salvianolic acid B (SalB). METHODS: Primary cultured rat cortical neurons were randomly divided into the control group, the model group and the SalB group. The cell model was established by oxygen/glucose deprivation for 3 h followed oxygen/glucose reintroduction for 24 h. The cortical neurons viability was determined by MTT assay. The leakage rate of lactate dehydrogenase (LDH) was measured by chromatometry. The mitochondrial membrane potentials (MMP) and the apoptosis rate were quantitatively analyzed by flow cytometry. The cytosolic free calcium was assessed using LSCM. The morphologic changes of neuronal nuclei were observed by Hoechst 33342 fluorescence staining. RESULTS: Compared to the model group, the cortical neurons viability, the survival rate and the fluorescence value of MMP in the SalB group were obviously increased (P<0.05,P<0.01). In addition, in the SalB group, the leakage rate of LDH, the fluorescence intensity of cytosolic free calcium and the apoptosis rate were significantly lower than those in the model group (P<0.01). CONCLUSION: The neuroprotective mechanism of SalB in the oxygen/glucose deprivation and reintroduction neurons would be due to the fact that SalB maintains the MMP and the calcium homeostasis.     

大白菜叶片长宽性状的QTL定位

利用一套大白菜双单倍体（DH）群体和包含287个分子标记的遗传图谱,运用复合区间作图法对叶长、无柄叶长、有柄叶长、叶宽、叶柄长、叶柄宽6个叶部形态学性状进行QTL定位及遗传效应的分析。结果表明：在4个连锁群上共检测到12个QTL,分别为叶长相关的3个,无柄叶长相关的2个,有柄叶长相关的2个,叶宽相关的1个,叶柄长相关的2个,叶柄宽相关的2个。各位点的遗传贡献率介于7.5 %~18.9 %之间。其中,叶长与叶柄长的QTL存在共定位,叶宽与叶柄宽的QTL也存在共定位。     

冀西北坝上地区几种蚜虫优势种对菜豆的侵害性研究

采用寄主转换方法研究了冀西北坝上地区几种蚜虫优势种对菜豆的适应性。结果表明,桃蚜、瓜蚜（棉蚜）和菊姬长管蚜转接到露地菜豆 的花、嫩尖、嫩叶等部位上仅能存活6~8 d,不能在菜豆上建立种群。室内寄主转换试验表明,取食菜豆嫩荚的蚜虫存活率明显低于取食茎部嫩尖的 蚜虫存活率。     

叶面喷施氨基酸水溶肥料对大白菜产量和VC含量的影响

以北京新3号大白菜为试验材料,在大白菜包心期喷施不同浓度氨基酸水溶肥料,测定大白菜产量及VC含量。结果表明：叶面喷施含氨基酸水溶肥料,大白菜产量提高5.8 %~12.4 %、VC含量增加15.8 %~51.7 %。     

不周土壤水分条件下萝卜幼苗叶片生理特性研究

在不同的土壤含水量条件下,测定了萝卜幼苗叶片的水分状况、叶绿素含量、光合速率、蒸腾速率及呼吸强度等指标.结果表明:随着土壤含水量的降低,萝卜幼苗叶片的相对含水量和水势下降,而且离体叶片的保水力在自然气干时下降较快;叶片叶绿素含量、光合速率、蒸腾速率和呼吸强度也随着土壤含水量的降低都减小.土壤含水量在20%～25%时,萝卜幼苗对土壤干旱的适应性最强.     

马铃薯氮素营养诊断研究进展

从外观诊断、化学诊断、无损测试技术3个方面,总结了目前马铃薯氮素营养诊断的若干方法.对各种方法的应用原理、发展现状进行了阐述,同时,对其他可用于马铃薯氮素营养诊断,但目前仍未采用的方法进行了简要的说明和论述.     

国家甜菜区试品种在甘肃河西走廊丰产与稳定性及抗病性评价

用品种稳定性参数及高稳系数（HSC）,对2004～2005年参加国家甜菜区试中的5个品种在甘肃武威市黄羊镇和酒泉地区的试验结果进行了综合分析,结果表明：BTS356的丰产、稳产性好．两年平均根产量和产糖量分别较对照陇糖2号增产40．62％和39．94％,差异达极显著水平,含糖率18．73％;对黄化病、白粉病、立枯病的抗性优于对照,具有较高的应用价值,适宜在甘肃省河西走廊生态条件下推广种植。     

饲用甜菜新品种“甜饲2号”的选育

饲用甜菜新品种“甜饲2号”是以甜菜雄性不育系G04A为母本,饲用甜菜自交系LC-1—1为父本,通过杂交选育而成的F1代杂交种。多点试验平均根产量为107751．8kg／hm^2,较时照增产2．3％;含糖率为13．7％,比对照高5．0度。2007年参加了生产示范试验,2008年底通过甘肃省农作物品种审定委员会认定命名。该品种经济产量高,蛋白质含量和含糖率较高,抗病性强,适应性广。