Low levels of haptoglobin and putative amino acid sequence in Taiwanese Lanyu miniature pigs

Porcine haptoglobin (Hp) is an acute phase protein. Its plasma level increases significantly during inflammation and infection. One of the main functions of Hp is to bind free hemoglobin (Hb) and inhibit its oxidative activity. In the present report, we studied the Hp phenotype of Taiwanese Lanyu miniature pigs (TLY minipigs; n=43) and found their Hp structure to be a homodimer (beta-alpha-alpha-beta) similar to human Hp 1-1. Interestingly, Western blot and high performance liquid chromatographic (HPLC) analysis showed that 25% of the TLY minipigs possessed low or no plasma Hp level (<0.05 mg/ml). The Hp cDNA of these TLY minipigs was then cloned, and the translated amino acid sequence was analyzed. No sequences were found to be deficient; they showed a 99.7% identity with domestic pigs (NP_999165). The mean overall Hp level of the TLY minipigs (0.21 +/- 0.25 mg/ml; n=43) determined by enzyme-linked immunosorbent assay (ELISA) was markedly lower than that of domestic pigs (0.78 +/- 0.45 mg/ml; p<0.001), while 25% of the TLY minipigs had an Hp level that was extremely low (<0.05 mg/ml). In addition, the initial recovery rate (first 40 min) in the circulation of infused fluorescein isothiocyanate (FITC)-Hb was significantly higher in the TLY minipigs with extremely low Hp levels than those with high levels. This data suggests that the low concentration of Hp-Hb complex is responsible for the higher recovery rate of Hb in the circulation. TLY minipigs have been used as an experimental model for cardiovascular diseases; whether they can be used as a model for inflammatory diseases, with Hp as a marker, remains a topic of interest. However, since the Hp level varies significantly among individual TLY minipigs, it is necessary to prescreen the Hp levels of the animals to minimize variation in the experimental baseline. The present study may provide a reference value for future use of the TLY minipig as an animal model for inflammation-associated diseases.     

蜂花粉抗化学性肝损伤研究进展

近年来研究发现蜂花粉具有显著的保肝功效,国内外学者已对此开展了广泛而深入的研究。本文就蜂花粉及活性成分对化学性肝损伤(四氯化碳肝损伤、酒精性肝损伤、氨基半乳糖诱导肝损伤)的作用效果和作用机理等方面的研究进展进行了综述,并对其研究方向和前景进行展望。     

西安地区鸵鸟养殖现状调查

简述了国内外鸵鸟养殖概况 ,通过对西安地区 5个公司的鸵鸟养殖场中的饲养管理、繁育、疾病防治及产品开发等的调查和分析 ,提出了西安地区在鸵鸟养殖方面存在的问题及我国鸵鸟养殖业的发展对策     

铜对意大利蜜蜂工蜂生理机能的影响

本研究旨在探索意大利蜜蜂工蜂成蜂饲粮中铜的添加水平对蜜蜂生长发育、生理生化及抗氧化性能的影响。从蜂群中随机取3000只刚出房的蜜蜂,随机平均分成6组,分别命名为A、B、C、D、E、F,每组5个重复,每个重复100只蜜蜂;6组工蜂幼虫分别饲喂铜的添加水平为0.00、2.00、4.00、6.00、8.00、10.00μg/g的糖水,饲养至全部死亡。分别取成蜂测定体成分、血淋巴生化指标、抗氧化指标、相关基因表达量、制作中肠切片,并测定寿命。结果表明:(1)铜对蜜蜂寿命的影响显著,从蜜蜂存活率曲线图来看,当饲粮中铜的添加水平为2.00μg/g时对蜜蜂中肠食膜的结构最为有利。(2)与对照组相比,铜的添加水平为2.00~10.00μg/g组9日龄蜜蜂体组织铜含量显著升高(P<0.05),蜜蜂血淋巴中总蛋白(TP)和总胆固醇(TCHO)含量显著降低(P<0.05)。(3)9日龄蜜蜂蜂体超氧化物歧化酶(Cu/Zn-SOD)活性随着铜添加水平的提高出现先升高后降低的趋势,在2.00μg/g时活性最高(P<0.05)。(4)当饲粮中铜的添加水平在0.00~10.00μg/g的范围内不断升高时,CAT、Ctr1基因相对表达量显著增加(P<0.05)。由此得出饲粮中铜的添加水平对蜜蜂的生理机能影响显著。     

甘肃省肃南县草地治蝗灭鼠工作经验

总结了肃南县１９５５年以来草原治蝗灭鼠为单一人工药物防治发展到生物新技术与新药物相结合的综合治理经验,提出了今后科技力量,开展多渠道,多学科,多部门协作防治的发展方向。     

伊维菌素微球在家兔体内的药动学

皮下注射伊维菌素 (IVM )微球悬液 (5 0mg/kg及 10 0mg/kg)和害获灭 (1%伊维菌素 ,0 5mg/kg) ,RP HPLC UV法定量 ,研究了IVM在家兔体内的药物动力学。害获灭皮下注射给药 ,药 时数据符合一级吸收一室开放模型 ,主要动力学参数为 :t1/2ka=7 2 4± 2 96h ;t1/2ke=36 38± 8 6 6h ;tmax=2 1 4 6± 4 82h ;Cmax=2 2 53± 2 32ng/ml;AUC =174 9± 318ng/1.h ,其动力学参数表现比较明显的个体差异 ,且与其它动物有明显差别。微球皮下注射一周后 ,血药浓度呈较稳定状态 ,到第 4 2天 (高剂量组 )和第 32天 (低剂量组 ) ,血浆中测不出H2 B1a(低于 2 5ng/ml)。以房室模型拟合 ,微球高低剂量组均符合有吸收二室开放模型 ,主要药动学参数均表现显著的个体差异。     

过瘤胃保护胆碱在反刍动物生产上的应用

过瘤胃保护胆碱(RPC),可直接添加到日粮中供饲反刍动物,不受瘤胃微生物降解。许多研究证明,RPC能提高反刍动物的生产性能改善其血液、肝脏代谢。本文简要介绍了过瘤胃保护胆碱对反刍动物的影响及作用机理,并对有待于继续研究的问题进行了探讨。     

乳铁蛋白的生物学功能及其基因工程研究进展

乳铁蛋白是一种具有多种生物活性的糖蛋白。本文主要就乳铁蛋白的生物学功能及其基因工程研究进展作一综述。     

巴比妥类镇静剂的检测研究进展

本文对巴比妥类安眠镇静剂检测方法的研究进展进行了综述。重点讨论了样品前处理方法及各种衍生化方法,GC、GC-MS、HPLC、HPLC-MS等检测方法。