甜玉米新品种‘浙甜12’

 ‘浙甜12’是以自交系‘GA1101’为母本,‘GA218’为父本杂交育成的超甜玉米新品种。平均鲜果穗产量为11 556 ~ 14 782.5 kg · hm^-2,穗长19.8 ~ 21.0 cm,穗粗5.0 cm,单穗质量262.0 ~ 279.7 g,籽粒可溶性糖含量26.6%,适口性好,商品性佳。     

Effects of noninvasive delayed limb ischemic preconditioning on prognosis of myocardial infarction

AIM: To study the effects of noninvasive delayed limb ischemia preconditioning (NDLIP) on animal cardiac function, myocardial morphology and myocardial apoptosis after myocardial infarction (MI). METHODS: Healthy SD male rats[n=45, weighing (250±10) g] were randomly divided into 3 groups:MI group:the animal model of MI was established by surgical ligation of left anterior descending artery (LAD) after 2 weeks; NDLIP group:after the success of the MI animal model, NDLIP was carried out every other day until the 4th, 6th and 8th weeks; sham group:as the negative control group, the animals were taken heart LAD threading but no ligation. All rats were fed conventionally. At the end of the 4th, 6th and 8th weeks, all rats were made ventricular intubation, and then the hemodynamic parameters were recorded. The blood samples were withdrawn from the abdominal aorta and the serum was separated via centrifugation. The serum contents of Bcl-2 and Bax were measured by ELISA. Left ventricular anterior wall was homogenized. The mitochondrial respiratory chain complexes Ⅰ, Ⅱ, Ⅲ and Ⅳ in the myocardial tissues were detected by ELISA. RESULTS: At the end of the 4th, 6th and 8th weeks, compared with MI group, left ventricular systolic pressure in NDLIP group was significantly increased, while left ventricular end-diastolic pressure in NDLIP group was significantly decreased (both P<0.05). Mitochondrial respiratory chain complexesⅠ, Ⅱ, Ⅲ and Ⅳ in NDLIP group were significantly increased (P<0.05). The serum level of Bcl-2 in NDLIP group was significantly increased and Bax level was reduced remarkably (both P<0.01). CONCLUSION: NDLIP improves the hemodynamic indexes, promotes the mitochondrial respiratory function and inhibits cell apoptosis, thus improving the prognosis of MI.     

Repressive effect of miRNA-363 via SOX4 on human osteosarcoma cell growth and apoptosis

AIM: To detect the expression of miRNA-363 and SOX4 in osteosarcoma tissues and to investigate the effect of miRNA-363 on the viability and apoptosis of human osteosarcoma cell line MG-63.METHODS: Real-time PCR was used to detect the expression level and the relationship of miRNA-363 and SOX4 mRNA in the osteosarcoma tissues and the corresponding paratumorous tissues collected from 63 patients. The expression levels of miRNA-363 and SOX4 in osteosarcoma cell line MG-63 after transfected with miRNA-363 mimics were measured. The cell viability was measured by CCK-8 assay. Flow cytometry was used to monitor the changes of cell cycle and apoptosis. The changes of SOX4 and miRNA-363 expression levels in the MG-63 cells after transfection with SOX4 siRNA or pcDNA/SOX4 was detect by real-time PCR.RESULTS: The expressed level of miRNA-363 was lower, and the expression level of SOX4 was higher in the osteosarcoma tissues than those in the adjacent normal tissues. A significantly negative correlation between the expression levels of miRNA-363 and SOX4 was observed. The expression of miRNA-363 in the MG-63 cells after transfection with miRNA-363 mimics was significantly up-regulated, while the expression of SOX4 in the MG-63 cells was significantly down-regulated, with significant difference as compared with the cells transfected with miRNA-NC and control cells. The viability of MG-63 cells was inhibited, the cell cycle was arrested in G₀/G₁ phase, and the cell apoptosis was increased by transfection with miRNA-363 mimics. The relative protein expression levels of SOX4 in SOX4 siRNA group and pcNDA/SOX4 group were significantly different from those in negative control group, but the relative expression levels of miRNA-363 had no significant difference. Over-expression of SOX4 restored the viability of the MG-63 cells reduced by miR-363.CONCLUSION: The expression level of miRNA-363 is low in human osteosarcoma tissue. miRNA-363 may inhibits the viability of osteosarcoma cell line MG-63 and promotes cell apoptosis in vitro via inhibiting the SOX4 expression.     

华南型黄瓜新品种‘力丰’

‘力丰’黄瓜是以耐热自交系‘冠农–863’为母本,抗病自交系‘揭阳乌皮青–311’为父本配制而成的杂交一代华南型新品种。植株生长势强,早熟性及丰产性好。瓜圆筒形,瓜长25 cm,单瓜质量400 g左右,品质良,风味佳,耐贮运。耐热,抗枯萎病,一般产量60.0 t·hm~(-2),适合华南地区春秋季及东南亚地区种植。     

六个猕猴桃品种在六盘水市的品比试验

以国内外较优6个猕猴桃品种为试材,利用引种技术进行品种的引进及筛选,综合评价各品种的物候期、适应性、果实品质等指标,以优化和调整六盘水猕猴桃品种结构,探明并确定贵州猕猴桃主产区六盘水市适应性较好的品种。结果表明:根据各品种的表现和"合理-满意度"分析,在六盘水表现较好的品种有"红阳",成熟期为8月中下旬,总糖含量为11.90%,口味香甜,果肉为红色,667m~2产量742.8kg,"金艳"成熟期为9月,667m~2产量1 018.6kg、单果质量118.43g、香味浓郁、果肉金黄色,"贵长"成熟期较晚,667m~2产量为1 401.7kg,香味较浓、果肉翠绿色;表现不好的品种有"西选2号""楚红",这2个品种产量均较低,果实口感一般;表现相对较好的品种有‘Hort 16A’口感较好,但抗病性较弱,"和平2号"单果质量156.80g,但667m~2产量仅有953.1kg,"海沃德"667m~2产量仅有703.6kg,表现不稳定。"红阳""金艳""贵长"在六盘水地区表现良好,适宜推广种植。     

不同枣头枝摘心方式对幼龄灰枣产量和品质的影响

以南疆地区密植灰枣为试材,采用生产上常用轻摘心、重摘心、极重摘心等3种枣头枝摘心方法,通过调查、测定枣吊数量、枣吊种类、果实单果质量、可溶性总糖、可滴定酸含量等指标,以确定一种合理的摘心方法。结果表明:枣头枝重摘心的单株坐果数显著高于轻摘心和极重摘心的单株坐果数,平均每株分别高出22.85、19.60个;重摘心后灰枣果实平均单果质量最大,为8.32g,分别比轻摘心和极重摘心灰枣果实的平均单果质量高9.5%、9.4%;枣头枝重摘心果实的可溶性总糖含量最高,为28.74%,分别比轻摘心和极重摘心后果实的可溶性总糖含量高3.86、4.13个百分点。因此,确定幼龄灰枣密植的夏季枣头枝摘心选择重摘心方式。     

不同授粉方法对设施番茄果实生长发育的影响

为探讨不同的授粉方法对设施番茄坐果率、单果质量和品质的影响,在番茄开花期分别采用自然授粉法、激素处理法、蜜蜂授粉法、机械振荡授粉法和熊蜂授粉法等5种方法进行授粉处理,测定了番茄坐果率、结果数量、单果质量、畸形果率和品质性状,比较不同授粉方式下番茄果实生长发育的差异,以期为设施番茄生产者选择适宜的授粉方法提供参考依据。结果表明:在同一穗位不同授粉方法对各穗坐果率均有显著影响,熊蜂授粉、蜜蜂授粉、机械振荡授粉和激素处理4种方法分别比自然授粉的坐果率提高25.46%、23.18%、19.62%和11.42%。不同辅助授粉方法能够有效增加番茄结果数量,各果穗上的表现为熊蜂授粉法蜜蜂授粉法机械振荡法激素处理法CK。熊蜂授粉番茄4~5穗果后期平均单果质量比其它处理及对照提高5.9%~11.8%,而且4种授粉方法番茄1~5穗果的畸形果率比自然授粉法降低9.14~47.00个百分点。各授粉方法的综合效果为熊蜂授粉法机械振荡授粉法蜜蜂授粉法激素处理法自然授粉法。为使设施番茄栽培达到优质高效的目的,并降低成本投入,推荐使用熊蜂授粉法。     

不同外源激素对越冬番茄坐果率、果实外观品质及产量的影响

越冬栽培番茄在低温弱光的生长环境下,常发生落花落果问题,施用外源激素可有效防治落花落果,选取4种外源激素为试材,以大果型、小果型2个番茄品种为研究对象,分别配制3种浓度对番茄进行蘸花处理,研究不同浓度激素对番茄坐果率、畸形果率及番茄果实外观品质的影响,以期筛选出较适合的外源激素及其适宜浓度。结果表明:整体上,对于小果型番茄,15mg·L~(-1)番茄灵理处的第3、4、5、6穗果的坐果率均较高,对于大果型番茄,各处理间第3、4穗果的坐果率均差异不显著(P0.05),整体上以20mg·L~(-1)的2,4-D、15mg·L~(-1)的番茄灵处理的坐果率较高,15mg·L~(-1)的番茄灵、15mg·L~(-1)的新保花保果乐处理的畸形果率为零。在大果型中,15mg·L~(-1)番茄灵处理的果实横径和纵径较大,10mg·L~(-1)的丰达处理果形指数较大,接近于椭圆形。小果型番茄果实横、纵径和果形指数均没有显著差异(P0.05)。20mg·L~(-1)2,4-D处理的大果型番茄产量较高,667m2产量为4 548.33kg,15mg·L~(-1)番茄灵处理的小果型产量较高,667m2产量为2 992.53kg。     

两种老鹳草属种子萌发特性研究

以草原老鹳草和毛蕊老鹳草为试材,采用不同种子处理方法、不同的萌发温度条件,测定2种老鹳草种子的发芽率、发芽势,研究老鹳草种子萌发特性,以期为老鹳草属植物的选育、繁殖提供参考。结果表明:不同老鹳草属种子在相同处理条件下的萌发率不同。2种老鹳草属种子萌发要求的最佳温度也不同。草原老鹳草种子发芽率最高是经砂纸摩擦处理组,为88.3%,发芽势为88.3%,最佳萌发温度为20℃。毛蕊老鹳草种子发芽率最高的是砂纸摩擦处理组,为86.6%,发芽势为86.6%,最佳萌发温度为25℃。     

桂林早熟花椰菜新品种比较试验

以本地品种瑞雪特大为对照,通过对7个花椰菜新品种的物候期、植物学性状、花球性状及产量等指标的鉴定及综合评价,筛选出适合桂林种植的早熟花椰菜品种。试验结果表明,新贵66号、花王60天植株健壮,长势旺盛,口感好,丰产明显,可进一步安排试验,进而示范推广。