三江源区四种栽培禾草抗寒性研究

通过对质膜相对透性、渗透调节物质、内源保护酶系等生理生化指标的测定,对三江源区引种栽培的4种禾草:青海草地早熟禾,青海冷地早熟禾,青海中华羊茅和无芒雀麦的抗寒性进行了研究。通过综合指标判定,4种牧草的抗寒能力为青海草地早熟禾最强,然后依次是青海冷地早熟禾,青海中华羊茅,无芒雀麦的最弱。     

Study on the Tissue Culture of Coleus blumei

This paper studied on the way of Coleus blumei, the leaf was chosen as explants and was inoculated on 1/2 MS medium with different combinations of 6-benzyladenine （6-BA）, α-naphthaleneacetic acid （NAA） and indole-3-butyric acid （IBA）. The optimal conditions of callus induction from explants were achieved on the medium containing 6-BA （2.0 mg· L^-1） and NAA （1.0 mg· L^-1）. Shoot tips were induced on the medium containing 6-BA （4.0 mg·L^-1） and NAA （0.5 mg·L^-1）. The same media conditions were found suitable for shoot multiplication, we multiplied shoots rooted best on 1/2 MS medium supplemented with IBA （0.1 mg·L^-1）.     

PRRSV tGP5基因克隆及pcDNA3.1-tGP5真核表达载体的构建

根据GenBank公布的猪繁殖与呼吸综合征病毒(PRRSV)S1株ORF5基因的核苷酸序列设计一对特异性引物,用PCR扩增PRRSV GP5蛋白非中和性表位缺失的tGP5基因,将基因定向连接在真核表达载体pcDNA3.1的HindⅢ和EcoRⅠ多克隆位点之间,构建重组质粒pcDNA3.1-tGP5。转化DH5α感受态大肠埃希菌,提取质粒。重组质粒经PCR、双酶切鉴定及DNA序列测定,成功构建了pcDNA3.1-tGP5基因的重组体。     

洪湖自然保护区浮游生物空间分布及与水质关系

研究围垸养殖对洪湖自然保护区水质的影响,从浮游生物视角评价水质现状及发展规律,以期为洪湖自然保护区科学管理及水生态修复提供基础数据。2019年6月在洪湖自然保护区不同方位水面布设10个采样点,开展水质及浮游动植物现状调查和分析,计算生物多样性,并与环境因子做CCA分析。调查共检出浮游植物6门57属74种,以蓝藻门的微囊藻、硅藻门的颗粒直链藻、绿藻门的单角盘星藻为主要优势种;浮游动物61种,以萼花臂尾轮虫和广布中剑水蚤为主要优势种。浮游植物Shannon-Wiener生物多样性指数均值为2.26,浮游动物均值为2.12,水质为中度污染状态,但有重度污染的趋势。CCA分析表明,浮游植物分布与SD呈正相关,与WT、COD_Mn等呈负相关;部分轮虫及大部分原生动物与SD、As呈正相关,与WT、Chl-a等呈负相关。洪湖自然保护区水体处于中富营养型状态。围垸养殖会导致洪湖水体有机物沉积、水质恶化,并对浮游生物种类组成和数量产生重要影响。     

Factors influencing seed reserve utilization during seedling establishment in maize inbred lines

Strong seedlings are essential for high yield. To explore the foundation of strong seedlings, we investigated various factors influencing the conversion and distribution of seed storage reserves during seedling establishment in maize inbred lines. Three maize inbred lines were used to explore the effects of seed size, seed vigor, illumination duration, temperature, water content, and salt concentration of the seedling medium on the utilization of seed storage reserves during seedling establishme...     

Mitochondrial DNA deletion of the brain tissue of aged rats with learning and memory deficit

AIM and METHODS: The ratio of mitochondrial DNA (mtDNA) deletion was measured to find the relationship between mtDNA deletion and aged learning and memory deficit. The aged rats were divided into two groups, aged learning and memory deficit group and aged learning and memory normal group. The ratio of mtDNA deletion was measured by dilution polymerase chain reaction. RESULTS: There are deleted mtDNA (about 4834 bp) in the cerebral cortex, hippocampus and cerebellum of both young and aged rats. The ratios of deleted mtDNA were similar in the cerebral cortex,hippocampus and cerebellum of young rats (about 0.00018%). The ratio mtDNA of aged learning and memory normal rats had increased by five-fold in the cerebral cortex and hippocampus, or one-fold in the cerebellum over young rats. The ratio of aged learning and memory dificit rats had increased by one-fold in the cerebral cortex or 0.8-fold in the hippocampus or two-fold in the cerebellum over aged learning and memory normal rats.CONCLUSIONS: There was really the increase of mtDNA in aging rat brain. And this increase was double in amount in aged learning and memory deficit rats compared to the normal learning and memory aged rats. It is suggested that the mtDNA deletions in the brain regions associated with learning and memory may be contributed to the cellular and molecular mechanism of learning and memory deicit with aged rats.     

玉米小斑病抗性种质鉴定及生理生化分析

以200份玉米优良自交系为材料,2022-2023年夏分别进行小斑病菌的田间接种,综合两年数据共鉴定出高抗小斑病的玉米自交系3份,分别为K22、CML50和齐319;筛选到包括Mo17在内的抗性玉米自交系24份。选取K22和B73两个具有不同抗性水平的玉米自交系进行小斑病菌的室内接种。结果表明,48 h和72 h时,自交系K22叶片的病斑数、病斑面积和病斑面积比等各项指标均显著优于自交系B73,进一步验证田间鉴定的准确性。48 h或72 h时,高抗小斑病自交系K22与感病对照B73相比,5种防御酶活性增加14.32%～70.45%。     

提高中文社科纸质型期刊利用率的管理探索

文章从三个方面对如何提高中文社科纸质型(或印刷型)期刊利用率的管理进行实践探索,取得良好效果进行阐述。     

玉米与小麦秸秆无土栽培基质的理化性状分析

将1～5cm的玉米与小麦秸秆,按质量添加15％鸡粪与1％尿素,堆制90d后风干,测定其理化性状。结果表明：两种秸秆单独作为栽培基质时存在物理性状的缺陷,体积质量偏小,大小孔隙比偏大;玉米秸秆基质pH值偏高,而小麦秸秆基质电导率（EC）偏大,两种秸秆混合后基质的pH值与EC在基质适宜指标的上限;秸秆基质的营养元素丰富,以全氮、钾和钙含量较高,两种秸秆基质间各种营养元素含量差异较小。但混合后基质的全氮、全磷、全钾及速效磷、速效钾含量均显著高于草炭;秸秆基质的砷、汞、铅与铜含量均在绿色食品生产要求范围内,镉的含量符合无公害蔬菜生产的要求。因此,合成无土栽培基质是农作物秸秆资源化利用的一条有效途径。