水肥耦合对设施番茄肥料减施效应的影响

为了探索水肥耦合在设施番茄上的应用效果及其对肥料减施的贡献,采用田间试验,设置6个处理,研究了水肥耦合条件下底肥减施20%和40%、灌溉量减少15%的条件下对设施番茄产量、品质和土壤养分的影响。结果表明:中水中肥(W2F2)处理产量最高,显著高于其他处理,产量比高水中肥(W1F2)处理提高2.0%,比常规处理(W1F1)提高4.1%。中水中肥处理番茄可溶性固形物、维生素C、土壤有机质、碱解氮、有效磷、速效钾含量比常规处理分别提高1.5%、5.8%、6.5%、6.3%、4.5%、10.6%。说明中水中肥处理(80%常规施肥+滴灌至田间持水量的85%,每公顷每次施用51kg大量元素水溶肥随水滴灌4次)不仅能提高设施番茄产量,还能在一定程度上提高番茄品质和土壤养分含量,是设施番茄肥料减施最佳处理。     

Effects of p21-activated kinase 6 on invasion and migration of human non-small-cell lung cancer A549 cells

AIM：To investigate the effects of p21-activated kinase 6 (PAK6) on the invasive and migratory abilities of human non-small-cell lung cancer A549 cells. METHODS：The expression of PAK6 mRNA in A549 cells, human bronchial epithelial (HBE) cells, non-small-cell lung cancer tissues and paired adjacent non-tumor tissues was measured by real-time PCR. After A549 cells were transfected with siRNA-PAK6 (siPAK6) or negative control (NC) for 48 h, the expression of PAK6 at mRNA and protein levels was measured by real-time PCR and Western blotting, respectively. The invasion and migration of A549 cells were detected by Matrigel invasion assay and Transwell migration assay. The cytoskeletal changes were observed with FITC-phalloidin staining under confocal microscope. RESULTS：The level of PAK6 mRNA in A549 cells was higher than that in HBE cells (3.50±1.16 vs 1.12±0.42, P<0.05). The level of PAK6 mRNA in non-small-cell lung cancer tissues was higher than that in paired adjacent non-tumor tissues (5.13±1.33 vs 1.08±0.37, P<0.05). The expression of PAK6 protein decreased by 72% in A549 cells transfected with siPAK6 (P<0.05), and the level of PAK6 mRNA significantly decreased in A549 cells transfected with siPAK6 (3.72±0.75 vs 0.69±0.21, P<0.05). Matrigel invasion assay and Transwell migration assay demonstrated that knockdown of PAK6 markedly attenuated the invasion and migration of A549 cells (P<0.05). The cytoskeletal actin remodeling and reduction of stress fibers in A549 cells transfected with siPAK6 were observed under confocal microscope. CONCLUSION：PAK6 may affect the invasive and migratory abilities of non-small-cell lung cancer cells by cytoskeletal actin remodeling.     

Effect of lansoprazole on gastric ulceration in rats

AIM:To investigate the effect of lansoprazole on gastric ulceration in rats. METHODS:Using the gastric ulcer model induced by hemorrhagic shock, restraint water-immersion stress and pylorus-ligature, the protective effect of lansoprazole (iv) on gastric ulceration was observed. RESULTS:Pretreatment with lansoprazole (7.5-60 mg/kg) significantly inhibited the formation of gastric ulcer in the three models in a dose-dependent manner. The autiulcer efficacy of lansoprazole was similar to that of omeprazole in the equal dose, but stronger than that of omeprazole for ulcer induced by water-immersion stress.CONCLUSION:The intravenously administered lansoprazole inhibited formation of experimental gastric ulcer in rats.     

RT-PCR技术诊断猪瘟的应用研究

应用反转录—聚合酶链反应(RT-PCR)对猪瘟进行诊断应用研究。应用RT-PCR况对来自广西不同地区的135份疑似猪瘟病料进行检测，以份诊断为阳性，阳性率62．2％。从百色、柳州地区等采集的健康猪扁桃体和淋巴结共276份，经RT-PCR检测，37份为阳性，阳性率为13．4％。其中健康猪扁桃体带毒较高，246份扁桃体中有35份阳性，占14．2％。采自柳州健康猪的26份淋巴结材料全为阴性，只有邕宁县的1份健猪淋巴结阳性。结果表明，RT-PCR技术可应用于猪瘟的临床诊断。     

小麦(T. aestivum)Waxy-D1 基因缺失材料的发现及分析

利用SDS-PAGE技术, 从河北省及其它省引进小麦地方品种900份中, 鉴定出了普通小麦Waxy-D1基因缺失材料一份, Waxy-B1基因缺失材料6份。 这些材料的发现, 为我国开展品质育种研究打下了基础, 尤其是Waxy-D1隐性材料尤为稀少, 是至今为止世界上发现的第二份含有该隐性基因的地方品种。 在小麦淀粉遗传控制研究和品质育种     

云南农产品加工业现状存在问题及对策

在深入分析云南省农产品加工业发展现状、存在问题的基础上,借鉴国内外农产品加工业先进地区的经验,提出了加快云南省农产品加工业发展的思路及对策建议。     

杂交水稻新组合赣优9812

赣优9812(赣香A/中种恢9812)是中国种子集团有限公司三亚分公司联合肇庆学院、江西省农业科学院水稻研究所选育的感温型三系杂交稻新组合,2018年2月通过广东省农作物品种审定委员会审定(粤审稻20180025)。该组合具有丰产性突出、米质较优、抗稻瘟病能力强等特点,适宜在广东省粤北以外稻作区早造、中南和西南稻作区的平原地区晚造种植。     

高压电晕电场处理紫花苜蓿的生物效应

为研究高压电晕电场生物效应物理机制,非均匀电场和电晕放电产生等离子体注入2种因素对种子的影响,利用频率为50 Hz,极距为4 cm,电压为0(ck)、4 kV、8 kV、12 kV、16 kV、19 kV的交流高压针-板电晕电场处理紫花苜蓿种子,通过1 mm厚的聚丙烯培养皿盖电介质阻挡和不加培养皿盖2组放电处理。培养后检测不同参数的高压电晕电场处理对紫花苜蓿种子生长的影响。结果表明:高压电晕电场处理紫花苜蓿种子时,无论是否有培养皿盖阻挡放电,均可改善紫花苜蓿种子的亲水性,使种子沉入培养皿底部,无培养皿盖阻挡放电组改善程度更大,除8 kV加盖阻挡组种子的吸水率比对照有所下降外,其余各组吸水率均上升。有无培养皿盖阻挡放电对紫花苜蓿种子的发芽势和发芽率有截然相反的影响,整个变化趋势呈非单调震荡型曲线,经培养皿盖阻挡放电后,可以非常有效的减小离子风对种子的物理刻蚀程度,同时降低紫花苜蓿种子接受的场强,并使紫花苜蓿种子接受的辐射电场更趋均匀。     

不同类型杜仲雄花茶活性成分的含量测定

采用紫外分光光度法和高效液相色谱法测定杜仲雄花茶中总黄酮、绿原酸及京尼平苷含量,探讨不同原料生产的杜仲雄花茶活性成分含量差异。结果表明,杜仲雄花茶中总黄酮、绿原酸及京尼平苷含量分别为1.89%～6.22%、0.06%～2.81%、0.06%～2.09%;不同类型杜仲雄花茶中各类活性成分含量有所差异,以花朵为原料生产的杜仲雄花茶中总黄酮含量和绿原酸含量均为最高,以花蕊为原料生产的杜仲雄花茶中京尼平苷含量最高,发酵杜仲雄花茶中各类活性成分含量均偏低。本研究可为开发不同保健功能杜仲雄花茶提供理论依据,有利于进一步推动杜仲雄花资源的开发利用。