Sequence Analysis and Prokaryotic Expression of NSP1 Gene of Bovine Rotavirus UK Strain

Rotavirus is a major cause of acute diarrhea in both many kinds of young animals and children under 5 years old.Rotavirus NSP1, a 55 ku RNA binding protein, is the product of gene 5, which can subvert innate immune responses and be one of virulent determinant factors.According to the sequence in GenBank, specific primers targeting to NSP1 gene were designed and the gene was amplified by RT-PCR, following by being cloned into the pET-28a(+) vector.It showed that the full length of NSP1 gene was 1 473 bp, encoding 491 amino acids.The NSP1 shared the highest identity with WC3 strain.The recombinant protein was induced in E.coli Rosetta(DE3) by IPTG and was analyzed by SDS-PAGE and Western blotting.The results revealed that NSP1 recombinant protein existed in the form of inclusion body with the molecular weight of 55 ku.The purified recombinant protein could be recognized by His-tag antibody.This study laid the foundation for further research on the relationship between the intracytoplasmic location of NSP1 protein and its activity.     

美国农产品分布对我国农产品区域发展的影响研究

美国农业一直处于领先地位,不仅得益于其丰富的自然资源和高科技的农业技术,其合理的农产品分布也不可少的影响因素。中国是一个农业大国,农业的发展离不开农产品,农产品的种植种类、农产品的区域分布、农产品的质量发展都直接影响农产品的发展。美国虽然面积和我国差不多,但因为有合理的布局,农产品贸易一直发展良好。要借鉴美国农产品的发展,研究和分析其农产品分布现状很有必要。     

Whistle signal variations among three Indo-Pacific humpback dolphin populations in the South China Sea: a combined effect of the Qiongzhou Strait's geographical barrier function and local ambient noise?

Geographic variations in the dolphin whistles could be useful in assessing association and isolation among populations. Whistle of free-ranging Indo-Pacific humpback dolphins (Sousa chinensis) among the Pearl River Estuary (PRE), Leizhou Bei (LZB) and Sanniang Bay (SNB) populations were investigated. A total of 2850 whistles with legible fundamental contour were extracted and 15 acoustic parameters were measured. Contrary to SNB, PRE and LZB had the same relative proportion of tonal type compositions with flat and sine representing the most frequent types. The generalized linear model analysis showed significant acoustic difference among populations and tonal types. All frequency parameters in SNB were significantly higher than those in PRE and LZB, where no significant variation was observed in most of the parameters either at the population level or within each tonal type. Canonical discriminant functions analysis showed a smaller difference between PRE and LZB than between PRE and SNB and between LZB and SNB. Compared with previous recordings, recent recordings demonstrated a consistent pattern of becoming higher in whistle frequency parameters in both LZB and SNB populations, suggesting that noise pollution in LZB and SNB increasing with time according to the acoustic niche hypothesis. Dolphin whistle's geographic variations could be shaped by the combined function of the geographical barrier function of the Qiongzhou strait and local ambient noise. Considering the isolated condition and the relatively smaller population size of the humpback dolphin in the SNB, more effective and proactive conservation actions should be taken to prevent the extinction of small populations.     

基于熵算法的股票指数高频数据复杂度测算与评价

在日内高频环境下检验基于兼容法的柯尔莫哥洛夫熵、样本熵和模糊熵等复杂度测算方法对我国沪深300股票指数的测算效率，并运用筛选后的有效算法分阶段研究和比较了序列复杂度的变化过程与变化幅度.结果表明，模糊熵算法是一种更适用于我国沪深300股票指数的有效复杂度测算方法，其对相似容忍度的敏感性更低，测度值连续性更好.随时间推移，我国沪深300股票指数复杂度整体呈上升趋势,而相较于发达市场甚至周边新兴市场其复杂度偏低.     

低聚木糖和低聚壳聚糖及其复合制剂对肉鸡生产性能、免疫机能和盲肠菌群的影响

本试验旨在研究低聚木糖(XOS)和低聚壳聚糖(COS)及其复合制剂对肉鸡生产性能、免疫机能和盲肠菌群的影响。将240只1日龄爱拔益加(AA)肉鸡随机分为5组,每组6个重复,每重复8只。对照组(CON)饲喂基础日粮,其他处理组分别在基础日粮中添加100 mg/kg XOS(XOS),30 mg/kg COS(COS),50 mg/kg XOS+10 mg/kg COS(XCⅠ),75 mg/kg XOS+15 mg/kg COS(XCⅡ)。结果表明:与对照组相比,XOS、XCⅠ、XCⅡ均能显著提高肉鸡21 d、42 d平均体重(BW)和1²1 d平均日增重(ADG)(P<0.05);XCⅠ、XCⅡ较COS 42 d体重和121 d平均日增重(ADG)(P<0.05);XCⅠ、XCⅡ较COS 42 d体重和1⁴2 d的ADG显著提高(P<0.05)。与对照组相比,XCⅠ、XCⅡ14 d、28 d肉鸡胸腺指数及14 d肉鸡十二指肠和空肠黏膜IgG含量;且XCⅠ14 d肉鸡盲肠乳酸杆菌数量较CON和XOS也有提高。结果提示,XOS和COS能改善肉鸡生产性能,改善肉鸡微生物区系,提高机体免疫功能,且XOS与COS低剂量复合添加效果优于单一寡糖,两者具有协同效应,本试验条件下以50 mg/kg XOS+10 mg/kg COS复合效果为最佳。     

基于快速幂法子空间跟踪的低频振荡辨识方法

基于中国农村1986~2010年的时间序列数据，采用状态空间模型和中介效应分析方法检验并测算了农村金融发展对农村贫困的影响效应及其成分。研究结果表明：农村金融发展有效促进了农村贫困缓减，并且在促进农村贫困缓减的过程中中介效应与直接效应并存。     

1961—2010年东北农作区春小麦需水量的时空变化特征

利用东北农作区58个气象站点1961—2010年的逐日气象数据,基于Simulation of Evapotranspiration of Applied Water(SIMETAW)模型分析气候变化背景下东北农作区春小麦生育期内作物需水量(Crop evapotranspiration,ETc)和灌溉需要量(Evapotranspiration of applied water,ETaw),以及典型站点春小麦的灌溉需求指数(Irrigation demand index,IDI)的时空变化特征。结果表明:近50年来,东北农作区日平均温度呈显著上升趋势,平均降水量下降趋势不明显,平均太阳辐射及作物蒸散量呈显著下降趋势。春小麦生育期作物需水量和灌溉需要量呈下降趋势,其分布均表现为西多东少。50年来春小麦作物生育期需水量下降主要集中于松辽及兴安岭南部地区,东部地区变化趋势不明显;生育期灌溉需要量下降集中于松辽及兴安岭地区,三江平原地区略有增加,长白山地区多年保持平稳。     

基于径向基神经网络的非寿险公司财务预警研究

选取我国保险行业中40家非寿险公司2006~2008年的混合非平衡面板数据作为研究样本，采用径向基神经网络模型从偿付能力、盈利能力、成长能力三个角度对非寿险公司财务预警进行研究。实证表明，此模型可以为管理层的财务预警提供更有有效的信息，并且在我国保险业具有样本量小，有效数据少的情况下，有着良好的运用前景。