Role of PPARα/PGC-1α in doxorubicin induced mouse dilated cardio-myopathy

AIM: To investigate the changes of peroxisome proliferator-activated receptors (PPAR)α/peroxisome proliferator activated receptor coactivator 1 alpha (PGC-1α) in doxorubicin (DOX) induced dilated cardiomyopathy (DCM) and its effect on the energy metabolism and myocardial function in mice. METHODS: Forty mice were randomly divided into 4 groups: control group, DOX group, PPARα inhibitor group and PPARα agonist group. The DCM model was established by injection of DOX. The protein levels of PPARα/PGC-1α were detected. The PPARα inhibitor and PPARα agonist were used 2 weeks beforeinjection of DOX. The contents of adenine acid and phosphocreatine (Pcr) in the mitochondria were measured by high-performance liquid chromatography (HPLC). The ANT activity was analyzed by the atractyloside-inhibitor stop technique. The changes of the echocardiography and hemodynamics were also observed. RESULTS: DOX induced DCM model was successfully established. The protein levels of PPARα and PGC-1α in control group were significantly higher than those in DOX group (P<0.05). Both of the high-energy phosphate contents and the transport activity of ANT were decreased in DOX group (P<0.05), and the hemodynamic parameters were disordered (P<0.01). Compared with DOX group, PPARα inhibitor pre-treatment significantly reduced the PPARα/PGC-1α expression. Meanwhile, high-energy phosphate contents in the mitochondria and the ANT transport activity of the mitochondria decreased, as well as the left ventricular function (P<0.05). On the other hand, PPARα agonist significantly increased the expression of PPARα and PGC-1α, and improved the transport activity of ANT. In addition, the hemodynamic parameters were ameliorated, but the high-energy phosphate contents of the mitochondria did not significantly change. CONCLUSION: PPARα/PGC-1α plays an important role in the regulation of ANT transport activity in dilated cardiomyopathy induced by DOX, and the activation of PPARα/PGC-1α has protective effects on the DCM induced by DOX.     

Juglone inhibits epithelial-mesenchymal transition of prostate cancer cells by regulating Wnt/β-catenin/Snail signaling pathway

AIM: To investigate the mechanism of juglone on epithelial-mesenchymal transition in prostate cancer cells. METHODS: Human prostate cancer LNCaP cells were divided into control group (without juglone), 12.5 μmol/L juglone group and 25 μmol/L juglone group. LNCaP cells in the latter 2 groups were treated with juglone for 24 h. The invasion ability of the LNCaP cells was detected by Transwell assay. The protein expression of E-cadherin, vimentin, Snail and β-catenin was determined by Western blot. The LNCaP cells were treated with LiCl and juglone in combination for 24 h, and the protein expression of Snail and E-cadherin was detected by Western blot.RESULTS: The results of Trans-well invasion assay showed that the invasion ability in juglone groups was significantly decreased (P<0.01). The protein expression of E-cadherin in the LNCaP cells treated with juglone was increased, and the expression levels of vimentin and β-catenin were reduced (P<0.01). Treatment with LiCl significantly attenuated the inhibitory effect of juglone on Snail expression and subsequent down-regulation of E-cadherin expression. CONCLUSION: Juglone inhibits the epithelial-mesenchymal transition by inhibiting the Wnt/β-catenin/Snail signaling pathway in the LNCaP cells.     

杨树大苗造林地膜覆盖技术

结合常规造林方法应用地膜覆盖技术,提高了杨树大苗造林成活率,加快了工程造林步伐,解决了东北地区春季大风、干旱、无雨的干燥恶劣天气条件下进行大规模工程造林成活率低的问题,达到一季造林、一次成活、一次成林。同时节省了造林资金。     

长沙市郊枫香人工林降水再分配及养分动态

采用定位试验研究方法对长沙市郊枫香人工林生态系统水文过程及养分动态进行了研究.结果表明：该森林生态系统春季平均降雨量为18.73 mm,其中以林冠穿透雨形式输入林地水量占降雨量的81.63%,以树干流形式输入林地水量占1.54%;树冠截留率随降雨量的增大而减小,平均树冠截留率为16.83%;在枫香人工林中,降水pH值的变化不大,且基本呈中性;养分元素质量浓度在各过程中变化不尽相同,Ca^2＋、K^＋质量浓度在穿透水和树干茎流中呈上升趋势,NH4^＋质量浓度在树干茎流中呈上升趋势,Zn^2＋、PO4^3-质量浓度呈下降趋势,Mg^2＋、Cu^2＋质量浓度变化不大.     

空气悬架对提高车辆抗倾翻能力的分析

针对近年来商用车侧翻事故的凸现,提出了一种利用空气悬架提高车辆倾翻阀值,减小车辆倾翻危险的方法,编写了控制器,在MATLAB中SIMULINK下建立了七自由度车辆模型,并进行了仿真。仿真结果表明,半主动空气悬架能够有效提高车辆倾翻阀值,提高车辆抗侧翻能力。     

病态池的检查和维修技术

随着沼气建设的快速发展,病态池的数量也随之增多。本文就如何分辨沼气池和输气线路病态、确定病态沼气池的病态类型、查找病态池漏水漏气部位及维修病态池提出了具体的方法。     

不同泡桐品种(种源)埋根苗生长与光合特性分析

为进一步研究泡桐埋根苗生长发育动态与光合特性之间的关系,以在郑州地区生长良好的4个泡桐品种(种源)埋根苗为研究材料,对1年生埋根苗生长期的苗高、地径、净光合速率、气孔导度、胞间CO2浓度、蒸腾速率等指标变化动态进行分析。结果表明:不同泡桐品种(种源)埋根苗的苗高生长和地径生长的季节变化趋势基本一致,苗高生长的快速生长期在7月中旬到8月下旬,9月为生长缓慢期,10月初到10月中旬苗高生长基本停止;地径生长的快速生长期从7月中旬到9月初,9月中下旬到10月为缓慢生长期,但10月下旬地径仍然还在缓慢生长,即泡桐地径生长比苗高生长延长10~15 d。不同泡桐品种(种源)埋根苗净光合速率、胞间CO2浓度、气孔导度、蒸腾速率4个光合指标在不同月份的日变化规律比较一致,胞间CO2浓度的日变化均呈现出先下降后上升的单谷曲线特征;净光合速率、蒸腾速率、气孔导度均在6、8、10月份出现双峰现象,且峰值出现时间较为一致,说明几个指标呈正相关;毛白33、9502的净光合速率相对较高,郑州白花的值相对较小,与生长量的表现一致;总体来看,5、10月份各泡桐品种(种源)无性系埋根苗的净光合速率偏低,6、7、8、9月份则相对较高,显示了泡桐在不同月份光合能力大小的差异。     

地下水环境质量评价Fuzzy-Grey模式

基于地下水环境质量评价所具有的模糊性和灰色性特点，将模糊数学理论中的隶属函数和隶属度等概念引入灰色系统灰色局势决策中，构成地下水环境质量的Fuzzy-Grey评价模式。用该模式对地下水环境质量进行评价，结果表明，该模式具有评价信息量多、结论符合客观实际等优点。     

基于反射率及导数的草原植被冠层光合有效吸收分量高光谱反演

利用ASD便携式野外光谱仪和光量子仪实测了6种草原植被类型关键生育期的反射光谱数据和光合有效辐射值,利用可见光波段处反射率及近红外波段区处一阶导数分别与fPAR建立逐步回归方程,同时,将各波段反射率与各波段导数光谱建立逐步回归方程.结果表明:典型草原光合有效辐射分量与可见光反射率相关性好于近红外波段反射率,其中在405和470 nm波段相关性最好;fPAR与一阶导数相关关系在855和965 nm波段处较强.fPAR与405和470 nm反射率以及965 nm一阶导数的多波段逐步回归分析结果取得了较单波段和NDVI最优的估算效果,R2达0.939.利用高光谱数据进行fPAR估算时,需要综合考虑可     

大豆抗旱的生理生态基础

大豆抗旱的生理基础和生态基础 ,前人已做过研究。综述前人在大豆抗旱的形态特征、解剖组织特点及影响抗旱的各生态因子方面的研究成果 ,为大豆的高产优质低耗栽培提供理论依据