排序方式: 共有49条查询结果,搜索用时 15 毫秒
31.
S. Zarei S. M. Taghavi H. Hamzehzarghani E. Osdaghi J.R. Lamichhane 《Plant pathology》2018,67(3):660-670
The population dynamics of Xanthomonas arboricola pv. pruni (Xap) and X. citri subsp. citri (Xcc) was assessed on over three dozen plant species/genotypes under field and greenhouse conditions. Both Xap and Xcc multiplied on red nightshade, black nightshade, bindweed, Chenopodium, common bean and wheat up to 20 days post‐inoculation (dpi) under greenhouse conditions. A high bacterial growth rate was observed on all (alfalfa, bindweed, Chenopodium, field mustard, millet and prickly lettuce) but one (liquorice) plant species tested under field conditions. Xap successfully proliferated on both lemon and sweet lemon up to 140 dpi, attaining a population density even higher than that of Xcc. The latter showed an increased growth rate on GxN, GF677, Ghisella 6 and Mariana 2624 rootstocks up to 140 dpi. While Xap and Xcc did not grow on pomegranate and common fig, they had a steady population growth on apple and pear plants up to 140 dpi, although the final population sizes were smaller than those observed on lemon and sweet lemon plants. The results suggest that a large number of non‐host plant species could support epiphytic populations of Xap or Xcc, which may have implications for plant disease epidemiology. 相似文献
32.
Pathogenicity and phylogenetic analysis of Clavibacter michiganensis strains associated with tomato plants in Iran
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E. Osdaghi M. Ansari S. M. Taghavi S. Zarei R. Koebnik J. R. Lamichhane 《Plant pathology》2018,67(4):957-970
During 2013–2016, 277 tomato fields were surveyed across Iran to monitor the status of bacterial canker of tomato, caused by Clavibacter michiganensis subsp. michiganensis. Altogether, 450 plant samples were collected, both with and without symptoms, from which 35 bacterial strains were recovered. These were positive for the PCR test performed using the Clavibacter‐specific primer pair CMR16F1/CMR16R1. Based on the phylogeny of the gyrB gene sequences, 31, three and one of the 35 strains were identified as C. michiganensis, Microbacterium sp. and Agrococcus sp., respectively. The 31 strains of C. michiganensis were further identified as C. michiganensis subsp. michiganensis (23 strains), C. michiganensis subsp. tessellarius (six strains) and Clavibacter spp. (two strains). This was subsequently confirmed by multilocus sequence analysis (MLSA) of five housekeeping genes (atpD, gyrB, ppk, recA and rpoB). In pathogenicity tests, all 23 strains induced wilting symptoms on tomato plants in greenhouse conditions, while no symptoms were observed on eggplant, bell pepper and chili pepper plants. All evaluated pathogenicity determinant genes (celA, pat‐1, tomA, ppaA, chpC and chpG) were detected in 18 out of 31 C. michiganensis strains, using eight specific primer pairs. Estimation of the number of nucleotide differences, sequence similarity matrix and MLSA clustered two peach‐coloured strains (Tom495 and Tom532) separately from all nine previously described subspecies, thereby suggesting these two strains are a new subspecies of C. michiganensis. However, a detailed taxonomic study using multiphased molecular approaches is needed to delineate a formal taxonomic name for these atypical strains. 相似文献
33.
Seyed Majid Ghoreishian Mohammad Norouzi Ali Fereydooni Saeed Nasser Toktam Asadolahi Neda Beigpour Maryamsadat Ghoreishian 《Fibers and Polymers》2017,18(7):1280-1287
In this study, central composite design (CCD) based on response surface methodology (RSM) was employed to optimize parameters of melt-spinning process of poly(ethylene terephthalate) partially oriented multifilament yarn (POMFY). On the basis of a four-variable CCD, RSM was used to determine the effects of spinning temperature, spinning pressure, take-up velocity and quenching air velocity on the levels of the elongation of POMFY as the response. The POMFY samples were also characterized by scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analysis. By applying a quadratic regression analysis, an equation indicating the effect of each variables on the response was developed. The predicted values of the parameters showed excellent agreement with the experimental values (R 2=0.9565, Adjusted-R 2=0.916). Moreover, the results confirmed that the CCD mathematical model was a suitable method to optimize the melt-spinning parameters of POMFY in an industrial scale. 相似文献
34.
Sara Sadat Aghabozorg Afjeh Jamal Shams Safar Hamednia Behzad Boshehri Asmaolhosna Amini Amin Omrani Mir Davood Omrani 《Iranian Biomedical Journal》2022,26(2):110
Background:Lithium is a therapeutic option for the treatment of the acute phase of the bipolar disorder and long-term management of this disorder. However, it is estimated that 10 to 60% of patients do not properly response to this medication.Methods:To investigate the role of MARK2 gene in response to lithium, we genotyped the MARK2 rs10792421 polymorphism in Iranian bipolar patients using ARMS-PCR.Results:Results of this study showed a significant association of this polymorphism with response to lithium. The A allele was more frequent in the responder than the non-responder group and also in the semi- responder group compared to the non-responder group in the codominant model of analysis. AA and AG genotypes were more frequent in both the responder and semi-responder groups compared to the non-responder group in dominant model of analysis.Conclusion:Based on the findings of the current study, the rs10792421 variant of MARK2 gene could be considered as a potential biomarker for predicting the treatment outcome of bipolar disorder type 1 in Iranian population. Key Words: Biomarkers, Bipolar, Lithium, Genotyping 相似文献
35.
Mohsen Naseri Mehdi Hedayati Maryam Sadat Daneshpour Fatemeh Bandarian Fereidoun Azizi 《Iranian Biomedical Journal》2015,19(3):172-176
Background:
The serum concentration of high-density lipoprotein cholesterol (HDL-C) is one of the important heritable risk factors for cardiovascular disease and is a target for therapeutic intervention. In this study, we aimed to evaluate the effects of lecithin cholesterol acyltransferase (LCAT) gene polymorphism rs5923 on LCAT enzyme activity and serum HDL-C concentration.Methods:
The study population was selected from consecutive individuals with HDL-C ≤ 5th percentile (n = 73) and extremely high HDL-C ≥ 95th percentile (n = 57) who had participated in the Tehran Lipid and Glucose Study. The rs5923 polymorphism was genotyped using direct sequencing. LCAT activity was measured by fluorometric assay kit, and lipid concentrations were measured using the enzymatic colorimetric method.Results:
The genotype frequencies were significantly different between the high HDL-C group (CC 94.7%, CT 5.3%) and the low HDL-C group (CC 83.6%, CT 16.4%) (P = 0.048). The T-allele frequencies in subjects with low and high HDL-C were 0.082 and 0.026, respectively (P = 0.16). The association of the single-nucleotide polymorphism rs5923 with low HDL-C was not statistically significant after adjustment for age, sex, and BMI (odd ratio = 2.65, 95% confidence interval = 0.32-21.5, P = 0.36, regression logistic analysis). Also, the effects of LCAT enzyme activity did not depend on the HDL-C level (P = 0.24).Conclusion:
rs5923 polymorphism is not associated with low HDL-C levels in Iranian population. Key Words: Polymorphism, Single nucleotide, Lipoproteins 相似文献36.
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38.
Fallah Mehrabadi Mohammad Hossein Ghalyanchilangeroudi Arash Ghafouri Seyed Ali Malekan Mohammad Ziafati Zahra Hosseini Hossein Mousavi Fatemeh Sadat Jabbarifakhr Masoumeh Aghaeean Leila 《Tropical animal health and production》2019,51(6):1737-1749
Tropical Animal Health and Production - Since 1998, Iran’s poultry industry has faced several outbreaks of low pathogenic avian influenza H9N2. Tissue samples were collected from a broiler... 相似文献
39.
Taghavi A Allan B Mutwiri G Van Kessel A Willson P Babiuk L Potter A Gomis S 《Avian diseases》2008,52(3):398-406
Oligodeoxynucleotides (ODN) containing cytosine-phosphodiester-guanine (CpG-ODN) motifs have been shown to stimulate the innate immune system against a variety of bacterial and protozoan infections in a variety of vertebrate species. The objective of this study was to investigate the immunostimulatory effect of CpG-ODN in neonatal broilers against Salmonella Typhimurium septicemia. Day-old broiler chicks, or embryonated eggs that had been incubated for 18 days, received 50 microg of CpG-ODN, 50 microg of non-CpG-ODN, or saline. Four days after exposure to CpG-ODN or day 2 posthatch, 1 x 10(6) or 1 x 10(7) colony-forming units (cfu) of a virulent isolate of Salmonella Typhimurium was inoculated by the subcutaneous route in the neck. Clinical signs, pathology, bacterial isolations from the air sacs, and mortality were observed for 10 days following challenge with Salmonella Typhimurium. The survival rate of birds in groups receiving either non-CpG-ODN or saline following Salmonella Typhimurium infection was 40%-45%. In contrast, birds receiving CpG-ODN had significantly higher survival rate of 80%-85% (P < 0.0001). Bacterial loads and pathology were low in groups treated with CpG-ODN compared to the groups receiving saline or non-CpG-ODN. Colony-forming units of Salmonella Typhimurium in the peripheral blood were significantly lower in birds treated with CpG-ODN compared to the group that received saline. This is the first time that CpG-ODN has been demonstrated to have an immunoprotective effect against an intracellular bacterial infection in neonatal broiler chickens following in ovo delivery. 相似文献
40.
Potential of willow and its genetically engineered associated bacteria to remediate mixed Cd and toluene contamination 总被引:1,自引:0,他引:1
Nele Weyens Kerim Schellingen Bram Beckers Jolien Janssen Reinhart Ceulemans Daniel van der Lelie Safiyh Taghavi Robert Carleer Jaco Vangronsveld 《Journal of Soils and Sediments》2013,13(1):176-188