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排序方式: 共有93条查询结果,搜索用时 593 毫秒
61.
Lowrance TC Loneragan GH Kunze DJ Platt TM Ives SE Scott HM Norby B Echeverry A Brashears MM 《American journal of veterinary research》2007,68(5):501-507
OBJECTIVE: To determine effects of administration of ceftiofur crystalline-free acid (CCFA) on antimicrobial susceptibility of Escherichia coli in feedlot cattle. ANIMALS: 61 feedlot steers. PROCEDURES: A cohort study was conducted. Steers were housed in pens (5 pens with 10 steers and 1 pen with 11 steers). Five steers in each pen were administered CCFA, and 5 served as control steers (1 pen had 6 control steers). The CCFA administration included a single-dose regimen (6.6 mg/kg, SC, on day 0), two-thirds-dose regimen (4.4 mg/kg, SC, on day 0), and 3-dose regimen (6.6 mg/kg, SC, on days 0, 6, and 13). Fecal samples were collected on days 0, 2, 6, 9, 13, 16, 20, and 28. Fecal samples were collected immediately before CCFA administration. Minimum inhibitory concentrations of 15 antimicrobials were determined for 3 E coli isolates/fecal sample. Escherichia coli were enumerated by use of direct-plating techniques. RESULTS: Resistance to 1 or more antimicrobials was detected in 986 of 1,441 (68.4%) isolates recovered. Administration of CCFA was associated with a transient increase in the population of ceftiofur-resistant isolates. Susceptibility returned to day 0 values (ie, samples collected immediately before CCFA administration) approximately 2 weeks after completion of CCFA administration. Agreement between ceftiofur resistance and co-resistance to ampicillin, chloramphenicol, streptomycin, sulfisoxazole, and tetracycline was almost perfect (kappa 0.97). We did not detect variation in susceptibility of E coli recovered from commingled control steers. CONCLUSIONS AND CLINICAL RELEVANCE: Administration of CCFA provided selection pressure that favored transient expansion of multiple-resistant variants. 相似文献
62.
Lores M Stryhn H McDuffee L Rose P Muirhead T 《American journal of veterinary research》2007,68(2):153-157
OBJECTIVE: To develop a transcutaneous ultrasonography (TUS) method for measuring the location of the stomach during various levels of fluid distension and evaluate any correlation between gastric fluid distension and stomach position. ANIMALS: 6 adult horses. PROCEDURES: Known volumes of water were administered in 2 trials. In trial 1, the stomach was evaluated prior to and after the administration of 2, 4, and 6 L of water. In trial 2, the stomach was evaluated after administration of 6, 8, 10, and 12 L of water. The TUS was performed at the 7th through 16th left intercostal spaces (ICSs). For each volume of water, an image was captured at the most dorsal point in each ICS where the dorsolateral aspect of the stomach wall was viewed. The distance between this point and a horizontal line drawn on the skin at the level of the elbow joint was measured. The measurements at all ICSs were used to estimate the gastric wall height at ICS 12, which was subsequently evaluated for statistical association with volume administered. RESULTS: Significant correlation between the estimated height of the stomach wall at ICS 12 and the volume of fluid administered was detected. A regression equation to estimate gastric fluid volume when initial values for gastric wall height (cm) at ICS 12 and fluid volume (L) are known was developed. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that use of TUS for gastric fluid volume estimation is a potentially useful technique. 相似文献
63.
Hezel A Bartges JW Kirk CA Cox S Geyer N Moyers T Hayes J 《Veterinary therapeutics : research in applied veterinary medicine》2007,8(4):247-254
Hydrochlorothiazide (1 mg/kg PO q12h) or placebo was administered to healthy cats for 2 weeks in a masked, placebo-controlled, crossover-design study, and 24-hour urine samples were collected. When cats received hydrochlorothiazide, 24-hour urine volume, ammonia, chloride, creatinine, magnesium, oxalic acid, phosphate, potassium, and sodium were significantly higher than when cats received placebo. Hydrochlorothiazide was associated with significantly lower urinary saturation for calcium oxalate, but no difference was found in 24-hour urine calcium and citrate, urinary saturation for struvite, or blood ionized calcium. Hydrochlorothiazide decreased urinary saturation for calcium oxalate and could be useful in managing cats with calcium oxalate uroliths. Results of this study, however, should not be extrapolated to cats that form calcium oxalate uroliths. 相似文献
64.
Incorporating biological information in local land-use decision making: designing a system for conservation planning 总被引:13,自引:0,他引:13
Theobald David M. Hobbs N.T. Bearly Tammy Zack Jim A. Shenk Tanya Riebsame William E. 《Landscape Ecology》2000,15(1):35-45
Human settlement is a formidable agent of change affecting fundamental ecological processes. Decisions governing these land-use changes occur almost exclusively at the local level and, as a result, they are made at many different locations and times. Consequently, it is difficult for ecologists to provide needed scientific support for these choices. We built an information system designed to support conservation decisions at local scales by offering data over the Internet. We collaborated with local stakeholders (e.g., developers, planners, politicians, land owners, environmental activists) to design the system. This collaboration produced several generalizations about effective design of information systems to support conservation. The most important of these is the idea that ecological data and analysis must be understood by those who will be affected by the decisions. Also, planning for conservation is a process that uses scientific data, but that ultimately depends on the expression of human values. A major challenge landscape ecologists face is to extend general landscape principles to provide specific scientific information needed for local land-use planning. 相似文献
65.
Nelson J. O’Driscoll Tammy Messier Michael D. Robertson John Murimboh 《Water, air, and soil pollution》2010,208(1-4):235-241
Global demand for carbon nanotubes (CNTs) is increasing dramatically. As CNTs become commonplace, the range of uses is expected to expand as will the potential for release into ecosystems. Recent research suggests that CNTs display increased suspension in water in the presence of natural organic matter (NOM), thus increasing their ability for transport and dispersion. However, it remains unclear how CNT size will affect the suspension of CNTs in natural waters. Here we examine the effect of CNT diameter (10–500 nm) and CNT length (1–40 μm) in the presence of 1% sodium docecyl sulfate (SDS), and two different freshwater NOM extracts on suspension of CNTs in water. Absorbance spectrometry (UV-VIS) was used to determine CNT concentration in solutions over a 68 h period in the dark. Seventy to ninety-five percent of the CNTs settled out of the 1% SDS solution as compared to 23–54% in each of the NOM solutions. The half-life of suspension in solution increased with decreasing CNT diameter (from 13.9 to 138.8 h?1 for solutions containing NOM). These results demonstrate that settling rates are strongly determined by NOM presence in solution as well as CNT size. 相似文献
66.
Pinchbeck LR Cole LK Hillier A Kowalski JJ Rajala-Schultz PJ Bannerman TL York S 《American journal of veterinary research》2006,67(8):1337-1346
OBJECTIVE: To determine whether staphylococcal isolates cultured from pustules and carriage sites in dogs with superficial bacterial folliculitis were genotypically the same strain by use of pulsed-field gel electrophoresis (PFGE). ANIMALS: 40 dogs with superficial bacterial folliculitis. PROCEDURES: Samples were obtained from 3 pustules and 3 carriage sites (anus, axillary skin, and nasal mucosa). Bacterial culture, morphologic identification, Gram staining, catalase and coagulase tests, speciation, and PFGE were performed. RESULTS: Of 246 isolates, 203 were Staphylococcus intermedius, 5 were Staphylococcus aureus, 15 were Staphylococcusspp, and 22 were coagulase-negative staphylococcal isolates. No dog had an isolate with the same PFGE pattern as an isolate from another dog. Coagulase-positive isolates from multiple pustules and multiple carriage sites had the same PFGE pattern in 37 of 39 (94.9%) and 22 of 39 (56.4%) dogs, respectively. Coagulase-positive staphylococcal isolates from at least 1 pustule had the same PFGE pattern as an isolate from at least 1 carriage site in 34 of 36 (94.4%) dogs. Ninety-seven of 116 (83.6%) coagulase-positive staphylococcal isolates from pustules had the same PFGE pattern as an isolate from at least 1 carriage site. Sixty-nine of 91 (75.8%) coagulase-positive staphylococcal isolates from carriage sites had the same PFGE pattern as an isolate from at least 1 pustule. CONCLUSIONS AND CLINICAL RELEVANCE: Coagulasepositive staphylococcal strains were heterogeneous among dogs with superficial bacterial folliculitis. In individual dogs, strains from multiple pustules were genotypically the same, and strains from pustules were genotypically the same as strains from carriage sites. 相似文献
67.
OBJECTIVE: To review the clinical presentation and histopathologic findings on a series of cats with orbital fibrotic disease and compare the data to that of humans with sclerosing orbital pseudotumor. ANIMALS: A retrospective study was undertaken, which identified tissue samples from seven cats between 1997 and 2002 with a history of orbital mass effect and pathology characterized by fibrous tissue proliferation. PROCEDURE: Information was obtained from medical records for affected cats, including age, sex, clinical signs, management, and outcome, with histopathology re-examined. RESULTS: Six of seven cats presented with unilateral orbital involvement that progressed to bilateral orbital disease despite treatment. Onset was insidious, evolving over weeks to months and was associated with fixation of orbital structures. Owners of six of the cats opted for euthanasia because of disease progression and pain. Histopathology of affected orbital tissue included extensive fibrosis with encapsulation of normal tissues without characteristics of neoplasia. CONCLUSIONS: Clinical findings and histopathology of globes and orbital tissues in cats bore many similarities to idiopathic sclerosing orbital pseudotumor in humans. In cats, the prognosis for the globe appears to be poor but an elucidation of the pathogenesis and earlier diagnosis coupled with more aggressive treatment modalities as indicated in humans may be beneficial. 相似文献
68.
Wen K Bui T Li G Liu F Li Y Kocher J Yuan L 《Comparative immunology, microbiology and infectious diseases》2012,35(4):289-301
We characterized immune modulating functions of porcine γδ T cell subsets in rotavirus infection using a gnotobiotic pig model of human rotavirus infection and sort-purified lymphocyte autologous co-cultures. We demonstrated that CD2+CD8- and CD2-CD8- γδ T cells have mainly pro-inflammatory function as evident by directly secreting IFN-γ or promoting CD4+ αβ T cell proliferation and IFN-γ production, whereas CD2+CD8+ γδ T cells mainly exert regulatory T cell function by expressing FoxP3, secreting IL-10 and TGF-β or increasing IL-10 and TGF-β production by CD4+ αβ T cells. γδ T cells responded to rotavirus infection by increasing TLR2, TLR3, TLR9 expression and IFN-γ and/or TGF-β production. The CD8- subsets likely differentiate into CD8+ subset by acquiring CD8 expression, explaining in part the apparently dual functions of CD2+CD8+ and CD2+CD8- subsets. Thus, both CD8+ and CD8- γδ T cell subsets can contribute to anti-rotavirus immunity and to the maintenance and restoration of intestinal and systemic homeostasis. 相似文献
69.
Objective To determine the complications and nonrecurrence rates following superficial lamellar keratectomy, bulbar conjunctivectomy, and adjunctive carbon dioxide (CO2) photoablation for corneolimbal squamous cell carcinoma (SCC) in the horse. Study design Retrospective study. Sample population Twenty‐four horses with corneolimbal SCC. Procedure Medical records of horses diagnosed with corneolimbal SCC that was surgically excised and where CO2 photoablation was used as an adjunctive therapy from 2000 to 2007 were reviewed. Signalment, prior therapy, tumor location and size, complications, and recurrence of SCC were recorded. Results The Thoroughbred was the most commonly (25%) represented breed. Lesions were >10 mm in diameter in 70.8% of cases. Eight horses (33.3%) had neoplastic cells extending to the deep margin of the keratectomy. All horses were available for follow‐up for an average ± standard deviation of 40.7 ± 25 months. Four horses (16.7%) developed a recurrence of SCC. Three of these four horses underwent repeat keratectomy and CO2 photoablation, one each, at 4 months, 1, and 2 years following the initial procedure. One horse underwent enucleation 8 months following the initial procedure. Conclusions and clinical relevance As an adjunctive therapy, CO2 photoablation was successful in 87.5% of the horses following a single procedure and in a total of 91.7% following a second therapeutic application. CO2 photoablation appears to be effective as an adjunctive therapy following removal of large corneolimbal SCC in the horse and in cases in which all tumor cells were not excised. 相似文献
70.
Habashi JP Doyle JJ Holm TM Aziz H Schoenhoff F Bedja D Chen Y Modiri AN Judge DP Dietz HC 《Science (New York, N.Y.)》2011,332(6027):361-365
Angiotensin II (AngII) mediates progression of aortic aneurysm, but the relative contribution of its type 1 (AT1) and type 2 (AT2) receptors remains unknown. We show that loss of AT2 expression accelerates the aberrant growth and rupture of the aorta in a mouse model of Marfan syndrome (MFS). The selective AT1 receptor blocker (ARB) losartan abrogated aneurysm progression in the mice; full protection required intact AT2 signaling. The angiotensin-converting enzyme inhibitor (ACEi) enalapril, which limits signaling through both receptors, was less effective. Both drugs attenuated canonical transforming growth factor-β (TGFβ) signaling in the aorta, but losartan uniquely inhibited TGFβ-mediated activation of extracellular signal-regulated kinase (ERK), by allowing continued signaling through AT2. These data highlight the protective nature of AT2 signaling and potentially inform the choice of therapies in MFS and related disorders. 相似文献