肾型传染性支气管炎病毒对鸡嘌呤代谢的影响研究

用T株肾型传染性支气管炎病毒(IBV)人工感染14日龄非免疫土种鸡建立感染模型,于攻毒前、攻毒后48 h、72 h、96 h、120 h 采集血样,测定血浆黄嘌呤氧化酶(xanthine oxidase, XOD)活性,探索鸡嘌呤代谢变化与肾型IBV致病机理之间的关系.结果表明,与攻毒前相比较, 攻毒后48 h和72 h血浆XOD活性明显增强, 差异显著(P<0.01,P<0.05).结果提示,XOD活性升高引起的嘌呤代谢紊乱可能是鸡肾型IB的病理机制之一.     

集雨栽培冬小麦田土壤水分和矿质氮

在半湿润易旱地区的关中红油土上,研究了垄上覆膜集雨栽培措施对冬小麦(Triticumaestivum)田土壤水分和矿质氮的影响。结果表明覆膜有一定的蓄水保墒效果,冬小麦返青期,0～40cm土壤贮水量覆膜处理比不覆膜处理平均多7.7mm;覆膜能使冬小麦在生长后期更有效地利用土壤深层贮水,在小麦成熟期,100～200cm土壤贮水量覆膜处理比未覆膜处理少23.8mm。覆膜有利于作物对氮肥的吸收利用,氮肥用量相同的处理,覆膜条件下的硝态氮浓度和矿质氮累积量低于不覆膜处理;施氮也可以明显增加土壤剖面中的硝态氮含量和矿质氮累积量。     

The relationship between high density lipoprotein subclasses and apoE gene polymorphism in obese subjects

AIM: The purpose of this study is to elucidate the relationship between apolipoprotein (apo) E polymorphism and plasma lipid profiles and HDL subclasses in obesity. METHODS: apoE genotype was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The subclasses of serum HDL in 93 obese subjects and 96 nonobese subjects were determined by two-dimensional gel electrophoresis conjunction with immunodetection method. RESULTS: apoE3/3 genotypes and allele ε3 frequency in obese group and the control group were both the highest, but no significant difference. In obese group, the genotype of apoE2 had higher serum apoE/aopCⅢ, HDL2a and lower apoB100, apoCⅢ, HDL3c levels compared with the genotype of apoE3 (P<0.05). In control group, the genotype of apoE2 had higher serum TC and apoE levels, but lower HDL3b level compared with the genotype of apoE3 (P<0.05). CONCLUSION: Polymorphism of the ApoE gene is associated with the distribution of HDL particles in obesity. Allele of ε2 carrier may slow the tendency of HDL particals shifted towards smaller sizes.     

应用PCR检测猪圆环病毒2型和猪繁殖呼吸综合征混合感染

猪圆环病毒病自1991年加拿大首次报道以来，包括我国在内的世界上许多国家和地区都报道有该病发生。近年来，该病发生呈上升趋势，给世界的养猪业造成了相当大的经济损失。目前猪圆环病毒在猪场中的感染率是很高的，它主要侵害断奶后2-3周的仔猪，发病率为20％。60％，死亡率为5％-35％。已成为阻碍当今养猪业发展的重要病原之一。     

完善动物免疫标识制度规范动物强制免疫工作

《动物免疫标识管理办法》由农业部于2002年7月1日颁布实施。我市自实行猪、牛、羊免疫标识制度以来．防疫工作取得了明显成效．进一步加强和规范了动物强制免疫工作。有效控制了重大动物疫病．促进了我市无疫区项目的规范建设和成果巩固。     

马铃薯纺锤块茎类病毒的检测和防治

马铃薯纺锤块茎类病毒病(potato spindle tuber viroid,PSTVd)是一种严重为害马铃薯生产的病害,降低产量20%—30%。防治的主要措施是应用无类病毒的种薯。由于目前还没有脱掉类病毒的有效措施,只能从未被饱和侵染的群体中鉴定筛选出未被侵染的个体,再脱掉其它病毒,作为核心繁殖材料。1987年以来,利用自制的电泳设备,以往复聚丙烯酰胺凝胶电泳法(return-polyacrylamide gel electrophoresis,R-PAGE)检测类病毒,筛选出未感病的个体,再用茎尖组织培养法脱掉其它病毒。经用马铃薯卷叶病毒等8种病毒酶标抗体鉴定筛选,获得既无类病毒也无主要马铃薯病毒的克新1、2、3和4号等主栽马铃薯品种的核心种。并已提供给省内外的良种场繁殖推广。1989和1990年抽样检测克山良种场繁殖的原种、一级和二级良种,未检测到类病毒。     

Environmental management and sustainable development in forested mountain areas of northeast China

IntroductionItiswellknownthatChinahasalonghistoryandcivilization,alargepopulationandlesscultivatedland.Thiskindofsituationisrelevanttotheresourcesdepletionandrecurrentnaturaldisasters.ForestsareveryimportantbothfortheeconomicalconstructionandfortheenvironmentalproteCtion.ThegraveinundationthatoccurredatsouthandnortheastriversintheSummerof1998givesusabitterlesson,manyscientistsandmassesbelievethatthelongandoverforestCuttingshouldberesponsibleforthecatastrophe.Theforestsonthemiddleandupperreac…     

寒地高效日光节能温室建造技术

黑龙江省地处北纬43°～53°以北高寒地区,过去由于冬季温度低,日照时间短,能源消耗大,冬季均以加温温室为主,随着市场经济和新一轮菜蓝子工程建设的发展,黑龙江省高效日光节能温室面积已近2000hm2(公顷),在冬春季节蔬菜生产中发挥着重要作用.     

玉米雄性不育突变体mi-ms-3的遗传分析及分子鉴定

Maize is one of the best crops in the utilization of heterosis. Male sterile lines are important germplasms for the hybrids production. A male sterile mutant named mi-ms-3 was obtained by screening in a mutator insertion library. The number of male anthers in tassel decreased and not exserted. There were few anthers with only two pollen sacs in the mutant tassels, and some of the anthers were degenerated to membranous and formed filaments at their ends. Although pollens in the anthers could be stained by I₂-KI, pollen shedding was abnormal and the number of pollen grains decreased. The number of silks in the ear of the mutant increased, and there was a sterile grain on both sides of the maturated kernel. Fertility of F₁ plants, which were obtained by hybridization between mi-ms-3 and maize inbred Mo17, was normal. Genetic analysis of F₂ population showed that the mutant phenotype was controlled by a recessive gene. The candidate gene was preliminarily mapped on the long arm of chromosome 3 by BSA and it was located between a SSR marker and an Indel marker with a distance of 1.5 cM. There are 21 candidate genes in this region. It was finally found that the insertion mutation of Mu transposon occurred at 30 bp upstream of the coding region of zm00001d042618 (zmm16) by transponson tagging and sequencing analysis. The results showed that mi-ms-3 was a new allele of sts1, which caused by a single base mutation in the coding region. RT-PCR analysis indicated that the expression of zmm16 in the mutant was decreased. The identification of the new allelic mutant of sts1 in this study would provide new materials for the study of flower development and hybrid seed production.