Generation of insulin-producing cells from PDX1 and NKX6.1 gene-modified bone marrow mesenchymal stem cells

AIM: To differentiate bone marrow mesenchymal stem cells (BMSCs) into functional insulin-producing cells to produce sufficient pancreatic islet cells for transplantation. METHODS: Recombinant adenovirus vectors carrying PDX1 and NKX6.1 genes were constructed and the bone marrow mesenchymal stem cells were infected by the recombinant adenovirus combined with several cytokines for differentiation. The expressions of PDX1, NKX6.1 and insulin and C-peptide in the differentiated bone marrow mesenchymal stem cells were detected by RT-PCR and Western blotting. After the differentiated bone marrow mesenchymal stem cells were transplanted into subrenal capsule of diabetic mice, cell morphology of the grafts as well as their secretion of insulin and C-peptide were detected. Besides, regulating capacities of grafts on the blood glucose level of the diabetic mice were also detected. RESULTS: BMSCs induced by recombinant adenovirus (pAdxsi-CMV-PDX1/CMV-NKX6.1) and several cytokines showed positive dithizone staining and the expressions of β-cell related molecule such as insulin and glucose transporter 2 were detected by RT-PCR, which showed a sustaining and stable expression. The similar results were showed by Western blotting, immunohistochemical staining and indirect immunofluorescence. The insulin secretions in the cells stimulated with glucose at concentrations of 5.5 and 25 mmol/L in the experimental group were (1 240.4±109.3) mU/L and (3 539.8±245.1) mU/L, respectively, and were significantly higher than those in control group. Moreover, transplantation of the cells to STZ mice in treatment group made serum glucose recover to normal level. CONCLUSION: PDX1 and NKX6.1 gene-modified bone marrow mesenchymal stem cells differentiate into insulin-producing cells in vitro. When these cells transplanted into STZ induced diabetic mice, their serum glucose could return to the normal level and they could live well. Thus this is a promising method for diabetes treatment.     

硬肉大果型番茄新品种北研1号的选育

北研1号是以05B-91为母本,以05B-93为父本配制而成的硬肉大果型番茄一代杂种。无限生长类型,中早熟,生育期 106 d（天）左右。成熟果红色,扁圆形,无绿肩,可溶性固形物含量4.8 %,VC 114.4 mg·kg^-1,可溶性总糖2.7 %。抗叶 霉病、ToMV,果实硬度0.84 kg·cm^-2,耐贮运,平均单果质量192.7 g,每667 m²产量6 000 kg左右,全国各地保护地、露 地均可栽培。     

不同来源菠菜品种营养品质分析与评价

为评价不同来源菠菜品种的营养品质,筛选出营养物质含量丰富,硝酸盐、单宁和草酸含量低的品种,测定了34份 菠菜材料的水分、VC、可溶性糖、粗纤维、草酸、单宁和硝酸盐7个品质指标的含量,并用模糊数学的隶属函数法对其营养品 质进行了综合分析。结果表明：不同菠菜品种在品质方面有较大的差异,34份菠菜材料中,用隶属函数法分析综合营养品质 最好的是欧洲的胜先锋、日本的トライ和アソナ,但在所有品种中,国内的京菠1号VC含量最高,达601.0 mg·kg^-1(FW),且 硝酸盐含量最低。     

华蔓茶藨子扦插繁殖技术研究

研究了华蔓茶藨子枝条不同部位、IBA不同浓度处理对扦插生根的影响.结果表明:插穗不同部位对愈伤组织出现期无影响,均为5 d,在生根时间上存在差异,上部16 d生根,比中部和基部提前2 d生根;对根质量的影响差异显著,上部最好,中部次之,下部最差;NAA和IBA对插穗生根均有明显促进作用,且IBA优于NAA,100 mg/L mA生根效果最好,生根率和生根质量均达到最高.     

干旱胁迫对果树开花的诱导及对生理生化性状的影响

干旱胁迫是许多热带、亚热带果树花芽诱导的有效措施,现就干旱胁迫对果树开花诱导的效果以及对果树生理生化过程的影响进行讨论.认为干旱胁迫明显降低果树的营养生长,影响果树体内碳水化合物以及含氮化合物的含量水平和相对比例,明显调节果树体内内源激素含量状况,并有效促进部分果树的开花.同时对干旱诱导的时间、强度以及今后控水促花研究中尚需解决的问题进行了讨论.     

西瓜、甜瓜果实品质及调控研究进展

西瓜甜瓜深受消费者喜爱,但是近年来品质呈现下降的趋势.西瓜甜瓜的品质包括外观品质、营养品质和风味品质,而品质的形成又受品种、土壤环境、肥料供应、水分供应、栽培方式、生长调节剂以及采收与贮运等因素的影响,所以要针对以上因素,从改进育种手段和栽培技术2大方面来改良和提高品质.     

秀珍菇生物富硒及富集水平

比较了不同采集地秀珍菇(Pleurotus geesteranus)子实体中硒含量、不同秀珍菇菌株对亚硒酸钠耐受能力和转化率,筛选富硒菌株与亚硒酸钠的最佳添加水平.结果表明,不同来源的子实体中硒含量存在差异;菌株XJ-1的耐硒能力与硒吸收转化率最高;液体培养XJ-1时,菌丝对亚硒酸钠的生理耐受敏感临界区间为3.0～5.0 μg/100 mL,亚硒酸钠添加水平在1.5～3.0 μg/mL时,菌丝对硒的转化水平最高.     

金耳菌丝体醇提物体外抗肿瘤抗病毒活性研究

采用体外肿瘤细胞株(小鼠白血病细胞株L1210、人乳腺癌细胞株MCF-7和人肠腺癌细胞株SW620)的增殖抑制试验模型和抗病毒(流感甲型病毒和疱疹病毒)的筛选模型,对3个金耳(Tremella aurantialba)菌株菌丝体醇提物活性进行比较研究.抗肿瘤试验结果表明,3种提取物材料在高浓度(200 μg/mL)时,对3个供试肿瘤细胞株的细胞增殖均有明显的抑制作用,其中以YK的抑制活性最高(对L1210、SW620和MCF-7的抑制率分别为89.4%,75.0%和64.1%);在中浓度(50 μg/mL)时,均表现出一定的抑制作用;而在低浓度(10 μg/mL)时,则均无明显的抑制作用.抗流感甲型病毒和抗疱疹病毒试验结果表明,只有YK对流感甲型病毒表现有抗病毒活性,但此活性较弱.     

水产养殖用水估价模型研究

水产养殖行业中,水资源利用效率低下,以及环境污染日益增加的主要原因,是水资源稀缺以及环境的消极外部影响的不合理体现所致.根据水产养殖用水的资源特性,水产养殖用水的评价模型已经建立起来了.该模型用水资源影子价格来确定资源水价的比例,用环境外部性来确定环境水价的比例.与此同时,探索和讨论养殖用水评价模型的途径和方法也已经构建起来了.运用水产养殖用水水价估值模型来计算2008年上海淡水池塘养殖用水的价格,其中资源水价部分(P1)为3.07元,环境水价部分(P2)为0.61元,折算系数(r)为0.95,计算得到每吨的价格为3.65元.     

稀土壳聚糖螯合盐对水产颗粒饲料性能的影响

研究了在饲料中添加稀土壳聚糖螯合盐（ＲＥＣＣ）对水产颗粒饲料性能的影响。根据鲫（Ｃａｒａｓｓｉｕｓａｕｒａｔｕｓ）鱼苗的营养需求配制４种类型的ＲＥＣＣ试验日粮,即１号饲料（０．００％）、２号饲料（０．０８％）、３号饲料（０．１６％）和４号饲料（０２４％）。测定４种饲料的粉化率和溶失率。结果显示,添加稀土壳聚糖螯合盐后可以降低饲料的 粉化率,饲料中添加０．１６％的稀土壳聚糖螯合盐效果尤其明显（Ｐ＜０．０１）;在饲料中添加ＲＥＣＣ可以显著降低饲料的溶失率（Ｐ＜０．０５）,并可以提高和改善水产颗粒饲料的性能。