中药对黄曲霉毒素B1中毒雏鸭肾组织抗氧化功能的影响

为观察黄曲霉毒素B1(Aflatoxin B1,AFB1)对雏鸭肾组织抗氧化功能的影响及复方中药对AFB1的颉颃效应,试验将90只7日龄雏鸭分为3组,每组30只.第Ⅰ组设为空白对照组,灌胃同等量二甲基亚砜;第Ⅱ、Ⅲ组为试验组,每天分别按0.1 mg/kg剂量给第Ⅱ、Ⅲ组雏鸭灌胃AFB1一次,连续投药21 d,试验期间给第Ⅲ组雏鸭日粮中添加2％复方中药.分别在给雏鸭投药后7、14、21 d,检测雏鸭肾组织总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、谷胱甘肽还原酶(GR)及丙二醛(MDA)等抗氧化指标.试验结果显示,第Ⅱ、Ⅲ组雏鸭肾组织SOD、CAT、GSH-Px及G1R活性与T-AOC均显著低于第Ⅰ组(P＜0.05),而MDA显著高于第Ⅰ组(P＜0.05);与第Ⅱ组相比,在日粮中添加复方中药的第Ⅲ组雏鸭肾组织各项抗氧化指标均得到明显的改善(P＜0.05).结果表明,黄曲霉毒素B1导致雏鸭肾组织抗氧化功能发生显著的变化,而复方中药能明显改善其变化.     

贵州香羊MSTN基因的多态性

肌肉生长抑制素（Myostatin,MSTN）是一种在骨骼肌中广泛存在的糖蛋白,是骨骼肌生长的负调控因子,其基因的缺失、插入或突变会引起肌细胞增生与肌纤维肥大从而提高产肉力。采用PCR-RFLP方法对67只贵州香羊MSTN基因进行了多态性分析。结果显示,5′UTR和外显子1没有出现变异,内含子1和外显子2中存在BspLⅠ、XmnⅠ、BmrⅠ、Hpy188Ⅰ4个酶切多态位点。纯合野生型（AA、CC、EE、MM）为优势基因型,杂合型（AB、CD、EF、MN）和纯合突变型（BB、DD、FF、NN）为非优势基因型,A、C、E、M等位基因为优势基因。     

镉重度超标耕地饲料桑利用安全性评价研究

通过试验研究筛选出生物产量高、营养成份丰富的饲料桑品种粤桑11号,在镉重度超标耕地桑叶中镉的含量符合国家饲料卫生标准。镉重度超标耕地桑叶发酵料中镉的含量也符合国家饲料卫生标准,在饲料桑基地用桑叶发酵料配合饲料饲养生猪其肉质指标测定结果符合食品要求。研究结果表明,在镉超标耕地上可种植桑品种粤桑11号进行饲料化利用。     

基于信息熵的天山山区降水时空变化特征

降水是山区气候中最重要的气象要素之一,其大小及变化直接关系到干旱山区中、下游的生态环境与经济社会的发展,但是,由于技术和地理条件的限制,是难以精确观测的气象要素。本研究采用天山山区31个台站和1960-2008年间的实测降水资料,应用信息熵方法对天山山区年、季和月的降水变化特征进行分析。结果表明,天山山区降水在时间和空间上均存在变异性,时间变异性表现为信息熵的无序指数(DI)年际变化小于季节变化,季节变化小于月变化。DI多年变化特征呈现下降趋势;冬季DI变化最为显著,夏季最小;冬季北坡降水随高程的增加DI变化呈显著增加趋势(P0.05),春、夏和秋季南坡降水随高程的增加DI变化呈显著减少趋势(P0.05)。山区月降水4-9月逐渐增加,10月至次年3月降水逐渐减少,月降水DI的变化与降水变化呈反相特征。在空间上DI变化与降水的分布相反,表现为南强北弱,东强西弱,伊犁河谷降水DI变化最小。结果证明,DI方法能够有效地分析山区降水的时空变化特征,为合理开发山区的水资源提供科学依据。     

太湖鹅群体遗传多样性研究

通过筛选的28个多态性较好的微卫星标记,检测了太湖鹅群体的遗传多样性,结果表明,28个微卫星位点均表现出多态性,总共检测到87个等位基因,平均等位基因数为3.1071个;群体多态信息含量为0.4450,杂合度为0.5206。瓶颈效应分析结果表明该群体已偏离了突变-漂移平衡,近期可能经历了瓶颈效应,种群数量曾经下降。根据遗传多样性检测数据,提出保护与开发建议。     

猪囊尾蚴头节单链抗体-PE40重组载体的构建及表达

从绿脓杆菌ATCC27853菌株中提取基因组,PCR扩增PE40基因,与pMD18-T栽体连接并转化感受态大肠杆菌JM109中,构建重组质粒pMD-PE40;经鉴定正确后将PE40基因插入抗猪囊尾蚴头节单链抗体基因重组质粒pMD-ScFv中,构建重组质粒pMD-ScFv-PE40,经酶切、电泳回收目的片段,并亚克隆到表达栽体pET-28a中,构建重组表达载体pET-ScFv-PE40;鉴定后转化E.coli BL21,IPTG诱导后进行SDS-PAGE和Western blotting验证.结果表明,试验成功构建了重组表达载体pET-ScFv-PE40,目的片段约为1800 bp;Western blotting证明重组蛋白具有一定的反应原性,为重组免疫毒素的制备及动物保护性试验奠定了基础.     

壳聚糖对仔猪生长性能及血清中类胰岛素生长因子-Ⅰ和生长激素水平的影响

本文旨在研究日粮中添加不同剂量壳聚糖对仔猪生产性能及血清中类胰岛素生长因子-I(IGF-I)和生长激素(GH)水平的影响。试验选择60日龄大白×长白×VI系杂交仔猪96头,随机分为4个日粮处理组,每个处理3个重复,每个重复8头,进行为期21 d的饲养试验。4种日粮分别是在基础日粮中添加0、0 .01 %、0 .05 %、0.1 %的壳聚糖配制而成。结果表明,壳聚糖对仔猪生长性能的影响呈二次剂量依赖关系,添加0 .01 %~0 .05 %壳聚糖能够提高仔猪平均日增重、平均日采食量及增重耗料比,并可提高仔猪血清中IGF-Ι和GH含量。而添加更高水平时生长性能却有下降趋势。因此,在本试验条件下初步得出,0 .01 %~0 .05 %为壳聚糖在仔猪日粮中的适宜添加量。     

Pathogen Analysis of Goat's Respiratory Diseases

To investigate the pathogens of goat's respiratory disease from a goat farm in Guangxi province, the epidemiological investigation, clinic observation, pathological examination, bacterium isolation and identification, biochemical test, PCR and animal experiment test were conducted.The preventive and control measures were taken on the basis of pathogen epidemiological characteristics and drug sensitive test results.The results showed that Mycoplasma, influenza virus and parasite were negative by isolation and culture or PCR, however, two strains of gram-negative bacteria named MS1 and MS2 were isolated from lung.MS1 was identified as Serratia marcescens by biochemical test and 16S rRNA sequencing, which shared 99% nucleotide homology with other Serratia marcescens in GenBank.And MS2 was identified as E.coil by the same methods that shared 99% nucleotide homology with other E.coli in GenBank.Animal experiment showed that two strains could cause death in mice.The drug sensitive tests showed two strains were highly sensitive to spectinomycin, amikacin, kanamicin and neomycin.Kanamycin and dexamethasone were used to treat the sick goats, and achieved good results.     

Study on Expression Difference of IGFBP-5 Gene in Different Tissues of Kazakh and Yanqi Horses

The study aimed to explore the mRNA expression pattern of insulin-like growth factor binding protein-5 (IGFBP-5) gene in different tissues of Kazakh and Yanqi horses.The expression of IGFBP-5 gene in different tissues of heart,liver,spleen,lung,kidney,small intestine,large intestine,cecum,intercostal muscles,longissimus dorsi muscles,brachialis muscle and gluteus in two horses were detected by Real-time quantitative PCR and compared the mRNA expression in the same tissues of two breeds.The results showed that the expression of IGFBP-5 in longissimus dorsi muscle and brachialis muscle of two breeds were significantly higher than other tissues including heart,liver,spleen,lung,kidney,small intestine,large intestine and cecum (P<0.05),and was the lowest in large intestine.The expression of IGFBP-5 in kidney,small intestine,large intestine,cecum,longissimus dorsi muscle and brachialis muscles of Yanqi horse were higher than in the same part of Kazakh horse,and among those in longissimus dorsi muscle and large intestine of Yanqi horse were extremely significantly higher than in the same part of Kazakh horse (P<0.01),and in small intestine and cecum of Yanqi horse were significantly higher than in the same part of Kazakh horse (P<0.05).The test was for further researching the biological function of IGFBP-5 gene,and it could provide a theoretical basis for genetic improvement of production performance of horse in our country.     

家蚕平附种母蛾微粒子病集团抽样检验方法改进研究

以超几何分布和二项分布为基本数学模型,综合现行家蚕母蛾微粒子病集团检验方法的优点,结合广西蚕种生产实际,以集团为单位,设计形成适合平附种的计数二次抽样检验方法。分别从理论和实际两个方面对新方案进行了比较分析。结果表明新方案解决了现行方法有毒集团与有毒蛾数不对等的问题,改进了农业行业标准散卯种方法过于严格,生产方风险过大的问题,改进了农业行业标准平附种方法过于宽松,使用方风险失控的问题,同时检验结果准确,检验工作量少,具有较高的准确性和较好的经济性。