丹东市林业资源综合分析

分析了丹东市林业资源现状;指出了目前存在的主要问题;提出了合理开发利用林业资源的措施。     

鲜食玉米设施栽培

针对近年来甜糯等鲜食玉米在我市种植面积迅速扩大的现状，本文根据田间试验点总结出鲜食玉米超早熟设施栽培技术。旨在为广大农民实际生产应用提供参考依据。     

黄麻（新、引进）品种在闽南地区的产量（鉴定）试验

通过对中国农科院麻类所新选育的3个黄麻圆果种和12个国外引进的黄麻优异品种进行2002-2005年品种比较试验，结果表明：C90-2、C90-6、C89-6比对照种粤圆五号增产14．97％-19．34％，均达极显著水平。O-3、O-1、O-3（红茎）比对照种宽叶长果增产32．02％-34．21％，均达极显著水平。O-4（Ⅱ）比对照种增产12．28％达显著水平。C-1、C-5比对照种粤圆五号增产11．90％和7．82％。且主要经济性状优良，抗病力强。黄麻品种C90-2、C90-6、C89-6、O-3、O-1、O-3（红茎）、0-4（Ⅱ）、C-1和C-5适合于福建闽南麻区推广种植。     

家蚕第2隐性赤蚁的遗传学研究——Ⅱ.ch-2基因的连锁分析

Ch-2基因是继ch之后新发现的一种隐性赤蚁ch-2基因与pM(2)、Ze(3)、L(4)、Pe(5)、E~(EL)(6)、q(7)、st(8)、I(9)、w-2(10)、ms(12)、cf(13)、U(14)、Se(15)、cts(16)、B_m(17)、nb(19)、rb(21)、or(22)、sp(23)、Nd(25)、及Y_m等各标志基因都是独立遗传.Ch-2与mln杂交F_2代分离+ch-2+min:ch-2+min:ch-2mln:ch-2mln=523:284:231:0≈2:1:1:0;ch-2与elp杂交F_2代分离+ch-2+elf:ch-2+elp:+ch-2elp:ch-2elp=219:97:68:0≈2:1:1:0,充分说明ch-2与mln、elp是连锁遗传的,即ch-2基因位于第18连锁群.     

Effects of FK506 on anti-glomerular basement membrane nephritis in rats

AIM:To investigate the effects of FK506 on anti-glomerular basement membrane(GBM) nephritis in rats. METHODS: Anti-GBM nephritis model was elaborated by rabbit anti-rat GBM antibody injection in SD rats in this study. The rats were divided into three groups: FK506 treated group(0.5 mg·kg^-1·d^-1, sc), untreated nephritis control group and normal control group. FK506 was administered daily six hours after injection of anti-GBM IgG. All the rats were observed urinary protein at the 4th day, the 14th day and the 21st day. At the same time, the kidney specimens were collected, and T cell transforming function was also monitored.RESULTS:Rats injected with rabbit anti-GBM Ab developed heavy proteinuria by 4 days, and serum creatinine and serum urea appeared which kept on the rising. Glmerular hypercellularity, crescents, and protein casts were observed in nephritic rats. By electron microscopy, the thickening of GBM and loss of foot processes were seen. T cell transforming function was higher than normal. But, all pathological changes obviously turned for the better in FK506 treated group. CONCLUSION:FK506 could inhibit the progression of rat anti-GBM nephritis.     

大白菜新品种琴萌2号的选育

琴萌 2号大白菜由两个自交不亲和系〔(青杂 3号 80 4×古青 76 2S 9S小 9S) 5S 1S 12×中青 3SI〕 2S 7S小 5S 2S 15S小 SI和秦白 2号 93 4S 5S小 12S小 11小 互为父母本杂交而成。该品种生育期 85d(天 ) ,每 6 6 7m2 净菜产量 6 0 0 0kg左右 ,高抗病毒病、霜霉病、软腐病 ,适宜沿海及黄河、长江中下游地区及辽宁、甘肃、宁夏等地种植。     

Preparation of gfp-bcl-XL-contained recombinant adenovirus vector by the homologous recombination in bacteria

AIM: To prepare gfp-bcl-X_L-contained recombinant adenovirus(rAd-gfp-bcl-X_L).METHODS: Bcl-X_L gene was amplified from pEGFP-C₃-bcl-X_L, subcloned into shuttle plasmid and formed transfer plasmid of pAdTrack-CMV-bcl-X_L. Then pAdTrack-CMV-bcl-X_L was linealinzed with PmeI and co-transformed into BJ5183 bacteria with adenovirus genomic plasmid of pAdEasy-1. The identified recombinant adenovirus plasmid was digested with PacI and transfected into 293 cells to package recombinant adenovirus particles. The target gene was detected by PCR.RESULTS: There were about 35% positive recombinant bacterial clones after the co-transformation of pAdTrack-CMV-bcl-X_L and pAdEasy-1 into BJ5183. Recombinant adenovirus particle were produced and further amplified after the transfection of pAdEasy-1-gfp-bcl-X_L into 293 cells. PCR test indicated that the recombinant Ad contained bcl-X_L gene. The titer of the purified rAd-gfp-bcl-X_L was 6.5×10¹² PFU/L. CONCLUSIONS: The homologous recombination in bacteria is a convenient and high efficient method to prepare rAd-gfp-bcl-X_L. This affords a good gene transfer vector for the gene therapy in human’s diseases.     

黄花蒿粗提物对几种害虫拒食性的初步研究

以黄花镐为原料进行浸提，经生物活性测定，结果表明黄花蒿Artenisia annua L.粗提物对供试的6种害虫均具有拒食性。其中对黑翅土白蚁Odontotermes formosanus Shiraki、赤拟谷盗Thibolium castaneum Herbst、谷蠹Rhizopertha dominica Fabricius拒食性极强，对棉蚜Aphis gossypii Glover、棉红蜘蛛Tetranychus urticae Koch及豇豆荚螟Etiella zinckenella Treitschke 也具有较强的巨食性。使用黄花蒿粗提物时，以稀释500倍和800倍效果最好，处理与对照之间有极显著差异。     

萝卜离体再生的影响因素

对影响萝卜离体再生的因素进行了研究,结果表明,不同基困型材料均以带柄子叶的再生频率最高;不同基因型材料所需的最适激素浓度和配比不同,在一定范围内提高6-BA浓度可促进不定芽的分化;苯基脲类细胞分裂素TDZ与嘌呤型细胞分裂素6-BA和KT相比,对促进萝卜不定芽分化的效果更好;AgNO_3可显著提高不定芽再生频率;NAA诱导生根的效果优于IAA。     

Antitumor effect of chlorophyllin in vitro

AIM: To study the effect and mechanism of chlorophyllin (CHL) inhibiting HT29 cells. METHODS: IC₅₀ value and growth curve of HT29 cells were detected with MTT method. Apoptosis was detected with Wright-Giemsa staining, FCM and DNA electrophoresis. Telomerase was detected by PCR-ELISA, and protein and mRNA expression of COX-2 gene were detected through RT-PCR and Western blot. RESULTS: CHL inhibited the growth of HT29 in a dose-dependent manner. CHL blocked HT29 cells in G₁ phase but did not induce apoptosis. Different concentration of CHL inhibits the expression of telomerase and COX-2 in HT29 cells. CONCLUSION: CHL inhibited the growth of HT29 cells by inhibiting the expression of telomerase and COX-2 and blocking cells in G₁ phase.