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61.
62.
Identification and characterization of a novel etiological agent of mango malformation disease in Mexico, Fusarium mexicanum sp. nov 总被引:1,自引:0,他引:1
Otero-Colina G Rodríguez-Alvarado G Fernández-Pavía S Maymon M Ploetz RC Aoki T O'Donnell K Freeman S 《Phytopathology》2010,100(11):1176-1184
The primary objective of this study was to characterize Fusarium spp. associated with the economically devastating mango malformation disease (MMD) in Mexico. In all, 142 Fusarium strains were isolated from symptomatic mango inflorescences and vegetative tissues in eight geographically diverse Mexican states from 2002 through 2007. Initially, all the Mexican isolates were screened for genetic diversity using appolymerase chain reaction and random amplified polymorphic DNA markers and were grouped into seven distinct genotypes. Based on results of these analyses, evolutionary relationships and species limits of the genetically diverse MMD-associated Fusarium spp. were investigated using multilocus DNA sequence data and phylogenetic species recognition. Maximum parsimony analyses of a five-locus data set comprising 5.8 kb of aligned DNA sequence data indicated that at least nine phylogenetically distinct Fusarium spp. within the Gibberella fujikuroi species complex are associated with MMD, including one species within the African clade (Fusarium pseudocircinatum), two species within the Asian clade (F. mangiferae and F. proliferatum), and at least six species within the American clade (F. sterilihyphosum and five undescribed Fusarium spp.). Molecular phylogenetic analyses indicate that a novel genealogically exclusive lineage within the American clade was the predominant MMD associate in Mexico. This new Fusarium sp. caused MMD and could be distinguished from all other known species morphologically by the production of mostly sterile, coiled hyphae which are typically associated with sporodochial conidiophores together with unbranched or sparsely branched aerial conidiophores. Koch's postulates were completed for isolates of the new species on nucellar seedlings of mango cv. Ataulfo. This pathogen is formally described herein as F. mexicanum. 相似文献
63.
64.
Canine atopic dermatitis is a multifaceted disease, whose clinical presentation may be affected by numerous factors, including the genetic background of the animal, the environment, the offending allergens and flare factors. In particular, breed-associated differences have often been mentioned but never defined precisely. Using a large data set of atopic dogs, we document in this study the clinical presentation of nine often-affected breeds and demonstrate the existence of substantial differences between the clinical phenotype of each breed and the whole population. Some of the differences may be due to genetic differences while others are most likely to be associated with variations in environmental factors. 相似文献
65.
66.
Soil population levels ofVerticillium dahliae in Ohio were monitored from May–October, 1982–1985, in 15 fields in potato-wheat rotation and two fields in potato monoculture. Population levels in fields in rotation ranged from 0 to 86 microsclerotia/10 g of air-dried soil with average values during each 6-month sampling period of 9.7, 12.9, 9.6 and 19.6 microsclerotia/10 g of soil for 1982, 1983, 1984 and 1985, respectively. In at least 2/3 of the fields sampled each year, soil populations ofV. dahliae peaked in either July or August, regardless of whether fields were under wheat rotation or potato monoculture. Of the 15 fields under potato-wheat rotation, 13 showed a general pattern of increased populations ofV. dahliae in one or both years following cropping to potato. Among those 13, significantly higher populations (P < 0.05) were observed in one wheat field in 1983 and in eight fields in 1985. In the two fields in potato monoculture, one consistently had population levels ofV. dahliae 3–4 times higher than any other field sampled in this study. Factors that may contribute to periodic changes inV. dahliae populations, implications of these changes in interpreting soil population data, and usefulness of a potato-wheat rotation in managingV. dahliae populations in Ohio are discussed. 相似文献
67.
Over 250 accessions ofSolanum phureja from Colombia and 300S. phureja—S. tuberosum hybrids were screened in the greenhouse for resistance toPseudomonas solanacearum (isolate K-60). Each plant was inoculated by stem puncture at the pre-bud stage and then held at a constant 28 C for 15 days. Seventeen clones with different levels of resistance to K-60 were inoculated with nine additional isolates representing all three races of the bacterium. The reactions of individual clones varied from resistance to almost all isolates, resistance to only one isolate, or complete susceptibility. Crosses amongS. phureja clones and betweenS. phureja and 24-chromosomeS. tuberosum stocks gave progeny with various percentages of resistant plants. Clones with resistance equal to that inS. phureja were found in theS. phureja × S. tuberosum crosses. Tests conducted in growth chambers showed that resistance was expressed more frequently at high light intensity (2000 ft-c) than at low intensities (1300 ft-c) at 24 C and 28 C. Resistance appears to be relatively simply inherited and the prospects for transferring a high level of resistance into cultivated forms are promising. 相似文献
68.
69.
The leaf phenolics of the potato speciesS. × ajanhuiri, S. stenotomum, S. megistacrolobum and the artificial F1 S. stenotomum × S. megistacrolobum hybrids have been compared using thin layer chromatography The wild potatoS. megistacrolobum produced unidentified phenolic compounds which were present in the artificialS. stenotomum × S. megistacrolobum hybrids and the YARI group ofS. × ajanhuiri, but which were absent inS. stenotomum. This further supports the hypothesis thatS. X ajanhuiri is of hybridogenic origin and was derived from natural crosses between the cultivatedS. stenotomum and the wildS. megistacrolobum. 相似文献
70.
Summary The effects of inbreeding in cultivated diploid potatoes were studied by using self-fertile hybrids from crosses between Group
Phureja and Group Stenotomum and haploids of Group Tuberosum. S1, S2 and S3 progenies usually exhibited decreased performance and vigour with increased inbreeding. In most families the inbreeding depression
seemed to coincide with a curve claculated on the basis of the expected loss of heterozygosity in a diploid organism. The
performance of some progenies deviated markedly from expectations apparently because a non-random sample of parents contributed
to later generations. Many inbred genotypes were lost owing to poor seedling vigour or tuberization. This indicated the presence
of many harmful recessive genes. The rapid increase in homozygosity following selfing at the diploid level provides a means
for developing homozygous clones with a potential for use in future genetic investigations.
Cooperative investigations of the Crops Research Division, Agricultural Research Service, U.S. Department of Agriculture, and the Wisconsin Agricultural Experiment Station. 相似文献
Zusammenfassung Inzucht bei kultivierten diploiden Kartoffeln wurde m?glich, nachdem man herausgefunden hatte, dass Kreuzungen zwischen kultivierten diploiden Arten und gewissen Haploiden vonSolanum tuberosum in hohem Masse selbstfertil sind. Die ursprüngliche genetische Basis dieses Versuches bestand aus 4 Familien, die durch Kreuzungen zwischen der Gruppe Phureja und der Gruppe Stenotomum mit der Gruppe Tuberosum haploidUS-W 4 erzielt wurden. Bei einer Anzahl der untersuchten Eigneschaften stimmte die durchschnittliche Inzuchtdepression der 4 Familien mit einer Kurve überein, die auf der Basis der Heterozygotie in einem diploiden Organismus (Tabellen 1, 2 und Abb. 1) berechnet wurde. Die vier Familien reagierten unterschiedlich auf die Einkreuzung (Tabelle 1). Dies kann teilweise auf genetische Unterschiede zwischen den ursprünglichen Elternpopulationen (S0) und auf eine Auslese auf Frohwüchsigkeit sowohl im geschlechtlichen als auch im ungeschlechtlichen Vermehrungszyklus zurückgeführt werden. Nur die kr?ftigeren Pflanzen blühten, und nur ein kleiner Teil davon war in den fortgeschrittenen Inzuchtgenerationen selbstfertil (Tabelle 4). Dies zeigt, dass Allele für Selbstunvertr?glichkeit in den Populationen noch vorhanden sind. Der niedrigere Grad der Selbstfertilit?t, ausgedrückt in Samen pro Frucht, weist beim Vergleich mit der Pollen- und der Eizellenfertilit?t darauf hin, dass rezessive lethale Gene vorhanden sind. Fortgeschrittene Inzuchtgenerationen wiesen eine abnehmende Knollenbildung auf (Tabelle 3). Beim Vergleich der Leistung der ersten Klongenerationen mit jener der ungeschlechtlichen Nachkommenschaften war die Depression in bezug auf die entsprechenden S0's bei den meisten Nachkommenschaften kleiner als bei ihren entsprechenden ungeschlechtlichen Elternpopulationen (Tabellen 1 und 2). Es wurden einige kr?ftige S5-Linien erzielt, manche von ihnen besitzen einen hohen Grad von Einheitlichkeit. Die rasche Abnahme der Frohwüchsigkeit nach der Einkreuzung deutet darauf hin, dass ein Heterosiseffekt bei Kreuzung fortgeschrittener Inzuchtklone erreicht werden k?nnte. Da Inzucht durch Selbstung eine Auslese auf Selbstfertilit?t erfordert, k?nnte dieses Vorgehen einige homozygote, selbstfertile Linien hervorbringen, was für eine Untersuchung der Genetik der Selbstfertilit?t sehr nützlich w?re.
Résumé L'autofécondation chez les pommes de terre cultivées diplo?des devint possible quand on découvrit que des croisements entre espèces cultivées diplo?des et certains haplo?des deSolanum tuberosum étaient hautement auto-fertiles. La base génétique initiale de la présente étude comprend 4 familles issues de croisements entre groupe Phureja et groupe Stenotomum avec le haplo?deUS-W4 du groupe Tuberosum. Pour un certain nombre de caractères, la dépression moyenne de consanguinité des 4 familles co?ncide avec une courbe calculée sur la base de l'hétérozygotie dans un organisme diplo?de (Tableaux 1, 2 et Fig. 1). Les 4 familles réagissaient différemment à l'autofécondation (Tableau 1). Ceci peut partiellement être attribué à des différences génétiques entre les populations parentales orignales (S0) et à une sélection pour la vigueur dans les cycles de reproduction à la fois sexuelle et asexuelle. Seules les plantes les plus vigoureuses fleurissaient, et seule une petite partie de celles-ci étaient autofertiles dans les générations successives autofécondées (Tableau 4). Ceci indique que les allèles d'autoincompatibilité sont toujours présents dans les populations. Le niveau plus faible d'autofertilité exprimée par le nombre de graines par fruit, quand on la compare à la fertilité du pollen et de l'ovule fait supposer que des gènes létaux récessifs sont présents. Les générations autofécondées successives montrent une tubérisation décroissante (Tableau 3). Quand on compare les performances des premières générations clonales avec les descendances assexuées, la dépression, comparée aux S0 respectifs, est plus faible chez la plupart des descendances que chez les populations parentales assexuées respectives (Tableaux 1 et 2). Plusieurs lignées S5 bien vigoureuses ont été obtenues, plusieurs de celles-ci montrant un haut degré d'uniformité. Le déclin rapide dans la vigueur après autofécondation suggère qu'on peut obtenir des hybrides vigoureux de croisements entre clones précédemment autofécondés. Puisque la consanguinité par autofécondation impose une sélection pour l'autofertilité, on peut de la sorte produire quelques lignées homozygotes auto-fertiles qui pourraient être utiles pour une recherche sur la génétique de l'autofertilité.
Cooperative investigations of the Crops Research Division, Agricultural Research Service, U.S. Department of Agriculture, and the Wisconsin Agricultural Experiment Station. 相似文献