Determination of the diglyceride content in greek virgin olive oils and some commercial olive oils by employing (31)P NMR spectroscopy

In this study, the diglyceride contents of 96 samples of virgin olive oils from the regions of Crete, Lesvos, Messinia, Pilion, Zakynthos, Halkidiki, and Ilia, 15 samples of commercial extra virgin and pure olive oils, and 3 samples each of refined olive oils and pomace oils were determined by a facile method introduced in a previous publication. This method is based on the phosphitylation of the free hydroxyls of the diglycerides with 2-chloro-4,4,5,5-tetramethyldioxaphospholane and the integration of the appropriate peaks in the (31)P NMR spectra. This preliminary study showed interesting trends in the diglyceride content of the virgin olive oils from the various regions of Greece that can be used as simple criteria to assess the olive oil characteristics. Analysis of variance has been carried out for the diglyceride content of each region in an attempt to detect possible differences in the diglyceride levels among the various regions. Finally, the relationship between the ratio of 1,2-diglycerides to the total amount of diglycerides and the total amount of diglycerides has been used to monitor the quality of virgin olive oils, commercial olive oils, refined olive oils, and pomace oils.     

Contribución al conocimiento de la neuro-arquitectura del ligamento periodontal dentario y su significatión funcional

Contribution to the study of the neuroardiitecture of the periodontal ligament and its functional significance In the present study particularly the specific receptors for certain sensory stimuli such as for pain, touch and pressure were studied. The vascular system is accompanied in its entirety by myelinated and nonmyelinated nerve fibers which form a kind of satellite system to the vessels.     

Near-infrared spectroscopy in saffron quality control: determination of chemical composition and geographical origin

Near-infrared reflectance spectroscopy has been applied for the first time to saffron spice to determine the chemical composition and geographical origin of 111 samples from the there main producers' countries: Iran, Greece, and Spain. The validation procedures with the results obtained by UV-vis and HPLC-DAD measurements demonstrated that this technique is appropriate to determine the following parameters: moisture and volatile content, coloring strength, (250 nm), and (330 nm), established on the ISO 3632 Technical Specification Normative and used to certify saffron quality in the international market. Also, it can be used to estimate the content of the five main crocetin glycosides, the compounds responsible for saffron color, the best correlations being for trans-crocetin di-(beta-D-gentibiosyl) ester (R2= 0.93), trans-crocetin (beta-D-glucosyl)-(beta-D-gentibiosyl) (R2= 0.94), and picrocrocin (R2= 0.92), the compound accepted as responsible for saffron bitterness. Finally, a discriminant analysis among the three geographical origins reveals that Iranian samples are the most different, whereas Greek and Spanish samples are more similar. All of these results reveal that NIRS spectroscopy has an enormous potential for its application to saffron quality control as the results are obtained in 2 min and without any sample manipulation.     

Influence of heat processing of African yam bean seed (Sphenostylis stenocarpa) flour on the growth and organ weights of rats

The influence of heat processing of African yam bean seed flour on the growth and organ weights of rats was studied. Body weight change, feed utilization and feed conversion ratio were improved by heat processing. All rats significantly (p0.05) gained weight except those fed raw African yam bean and basal diets (diets 3 and 1 respectively). Raw African yam bean diet decreased the growth of rats and had negative effect on the organ weights especially the pancreas which was enlarged. The results indicate that heat processing improved the growth of rats and organ weights due to heat inactivation of toxic factors especially trypsin inhibitors.     

The Effects of the Decaffeination of Coffee Samples on Platelet Aggregation in Hyperlipidemic Rats

The effect of coffee on cardiovascular diseases is still controversial. It is known that the process of decaffeination may influence the chemical constitution and, therefore, the biological effects of coffee. This study thus evaluated the effects of decaffeination on the levels of total phenols and chlorogenic acids in Coffea arabica L. samples, as well as the effects of ingesting both integral and decaffeinated coffee on the lipid profile and hemostatic and hematological parameters in normal and hyperlipidemic rats. Samples of integral and decaffeinated lyophilized coffee (Coffea arabica L., planted in Brazil) were used for chemical analysis (total phenols, chlorogenic acid and caffeine contents). For the bioassays, coffee beverages were prepared with non-lyophilized samples (10 % w/v) and were filtered and administered to animals by gavage (7.2 mL/kg/day) over 30 days. On the 31st day after beginning the treatment with coffee beverages, hyperlipidemia was induced to the animals by administering Triton WR-1339 (300 mg/kg body weight). On day 32, blood was taken to determine the lipid profile, platelet aggregation, prothrombin time, partially activated thromboplastin time and hemogram. The contents of both phenolic compounds and chlorogenic acid in the integral coffee beverage were significantly lower than those in the decaffeinated coffee beverage. The animals treated with Triton WR-1339 presented a mixed hyperlipidemia. Although the decaffeination process caused a relative increase in total phenols and chlorogenic acids, the coffee drinks were unable to change the lipid profile or the hemostatic and hematological parameters in the studied animals.     

Effects of the Consumption of Caffeinated and Decaffeinated Instant Coffee Beverages on Oxidative Stress Induced by Strenuous Exercise in Rats

Many authors attribute the antioxidant activity of brewed coffee to its caffeine content. In addition, caffeine intake has been associated with increased performance during physical exercise. This study analyzed the in vivo effects of drinking caffeinated and decaffeinated instant coffee (8%, w/v) on oxidative stress and antioxidant enzyme activity in the anterior tibialis muscles of rats subjected to intense exercise. It was observed that exercise induced lipid peroxidation (estimated using malondialdehyde) and protein oxidation (evaluated by determining the formation of carbonyl groups) in the muscle (P < 0.05). Decaffeinated instant coffee and caffeine solution did not exhibit antioxidant activity in vivo. Caffeinated instant coffee beverage intake did not induce changes in superoxide dismutase and glutathione peroxidase activities but was able to diminish lipid and protein oxidation in the anterior tibialis muscles of rats after exercise (P < 0.05), contributing to a reduction in the oxidative stress triggered by exercise.     

Uptake of soil mineral nitrogen by Acacia mangium and Eucalyptus urophylla × grandis: No difference in N form preference

The introduction of N₂‐fixing tree species in fast growing tree plantations is a sustainable management option aiming to reduce the risk of nitrogen (N) deficiency due to a large and frequent exportation of nutrients at harvest. Differences in soil mineral N preferences between Eucalyptus urophylla × grandis and Acacia mangium may, in addition to facilitation related to atmospheric N₂ fixation, contribute to the success of mixed‐species plantations of the two species on nutrient‐poor soils of the coastal Congolese plains. We tested whether these two species differ in their preference for nitrate or ammonium by supplying either ammonium or nitrate enriched in ¹⁵N to six‐month‐old potted trees growing in an open‐air nursery. Although the uptake of nitrate tended to be higher than that of ammonium by both species, the difference was not significant and there was no significant difference between the species regarding their preferred form of soil mineral N. Despite much lower N contents in foliage, stems, and roots of eucalypt compared to acacia, the specific rates of N uptake were up to three times higher for eucalypt than acacia, which suggests that atmospheric N₂ was the major source of N in the six‐month‐old acacias. We conclude that N₂ fixation rather than complementarity for soil mineral nitrogen alleviates the competition between species in successful mixed eucalypt and acacia plantations.