全文获取类型
收费全文 | 1106篇 |
免费 | 61篇 |
专业分类
林业 | 63篇 |
农学 | 29篇 |
基础科学 | 2篇 |
223篇 | |
综合类 | 73篇 |
农作物 | 56篇 |
水产渔业 | 98篇 |
畜牧兽医 | 540篇 |
园艺 | 34篇 |
植物保护 | 49篇 |
出版年
2023年 | 9篇 |
2022年 | 13篇 |
2021年 | 28篇 |
2020年 | 25篇 |
2019年 | 23篇 |
2018年 | 33篇 |
2017年 | 30篇 |
2016年 | 21篇 |
2015年 | 37篇 |
2014年 | 27篇 |
2013年 | 49篇 |
2012年 | 52篇 |
2011年 | 75篇 |
2010年 | 42篇 |
2009年 | 43篇 |
2008年 | 56篇 |
2007年 | 52篇 |
2006年 | 48篇 |
2005年 | 42篇 |
2004年 | 49篇 |
2003年 | 32篇 |
2002年 | 46篇 |
2001年 | 34篇 |
2000年 | 34篇 |
1999年 | 20篇 |
1995年 | 6篇 |
1994年 | 7篇 |
1993年 | 6篇 |
1992年 | 7篇 |
1991年 | 10篇 |
1990年 | 8篇 |
1989年 | 10篇 |
1988年 | 16篇 |
1986年 | 11篇 |
1985年 | 8篇 |
1984年 | 8篇 |
1983年 | 7篇 |
1982年 | 6篇 |
1981年 | 7篇 |
1979年 | 6篇 |
1978年 | 11篇 |
1977年 | 12篇 |
1975年 | 6篇 |
1974年 | 6篇 |
1973年 | 6篇 |
1972年 | 6篇 |
1971年 | 6篇 |
1969年 | 6篇 |
1968年 | 8篇 |
1966年 | 8篇 |
排序方式: 共有1167条查询结果,搜索用时 0 毫秒
991.
Regulation of the germinal center response by microRNA-155 总被引:1,自引:0,他引:1
Thai TH Calado DP Casola S Ansel KM Xiao C Xue Y Murphy A Frendewey D Valenzuela D Kutok JL Schmidt-Supprian M Rajewsky N Yancopoulos G Rao A Rajewsky K 《Science (New York, N.Y.)》2007,316(5824):604-608
MicroRNAs are small RNA species involved in biological control at multiple levels. Using genetic deletion and transgenic approaches, we show that the evolutionarily conserved microRNA-155 (miR-155) has an important role in the mammalian immune system, specifically in regulating T helper cell differentiation and the germinal center reaction to produce an optimal T cell-dependent antibody response. miR-155 exerts this control, at least in part, by regulating cytokine production. These results also suggest that individual microRNAs can exert critical control over mammalian differentiation processes in vivo. 相似文献
992.
Colchicine alters the nerve birefringence response 总被引:2,自引:0,他引:2
The internal perfusion of squid axons with colchicine reversibly and selectively reduces the transient sodium current and the birefringence response to a brief depolarizing voltage pulse. 相似文献
993.
994.
Adami C Di Palma S Gendron K Sigrist N 《Journal of the American Animal Hospital Association》2011,47(6):436-442
Two healthy cats underwent elective surgical procedures under general anesthesia. One developed severe esophagitis leading to esophageal rupture, mediastinitis, and pyothorax. The other cat developed esophageal stricture, diverticulum formation, and suspected iatrogenic perforation. Both cats had signs of dysphagia and regurgitation beginning a few days after anesthesia. The first cat also had severe dyspnea due to septic pleural effusion and pneumomediastinum. In the second cat, endoscopy revealed diffuse esophagitis, an esophageal stricture, and a large esophageal diverticulum. Rupture of the esophageal wall occurred while inflating the esophagus for inspection. Due to the poor prognosis, both cats were euthanized. Necropsy revealed severe esophageal changes. Postanesthetic esophagitis has been previously described in dogs and cats; however, severe life-threatening esophageal injuries rarely occur as a sequel to general anesthesia. To the authors' knowledge, esophageal rupture secondary to perianesthetic reflux has never been reported in cats. 相似文献
995.
996.
Ms K. Löfkvist R. Larsen J.-E. Englund B. W. Alsanius 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(4):326-334
Abstract Irrigation strategies in pot plants of Kalanchoë blossfeldiana were studied in two commercial greenhouse nurseries by continuous weighing of plants on a high-precision balance. The objectives were to study actual irrigation strategies implemented by growers and to evaluate the method of continuous weighing as a potential tool for future irrigation management. Mean values of temperature, relative humidity, light, and weight were recorded every five minutes using data loggers. The change in weight over time was estimated and related to the calculated total canopy surface area of the studied plants. The rate of weight change was then statistically tested in relation to the recorded external factors. The factor with the strongest effect on total water-consumption rate over time was the light integral. As expected, water consumption was also affected by temperature and humidity, as well as the time of day. Although the growers indicated clear irrigation strategies, the study showed that these were not implemented in a true sense or correlated to the information available in greenhouse climate computers. The study also indicated that a high-precision weighing balance might be an important tool for future control of plant growth and plant architecture through irrigation in the pot plant industry. 相似文献
997.
Stefania Astolfi Sabrina Zuchi Stefano Cesco Zeno Varanini Roberto Pinton 《Soil Science and Plant Nutrition》2013,59(7):1079-1083
Abstract Recent research has evidenced a relationship between Fe nutrition and S nutrition. Aim of the present work was to investigate the effect of Fe deficiency on the capacity of maize roots to take up and metabolize S. Maize (Zea mays L. cv. Cecilia) plants were grown for 10 d in nutrient solution (NS) with (+S) or without (?S) sulphate and Fe was added as FeIII-EDTA at 80 μm. After removing the extraplasmatic Fe pool, half of the plants of each treatment (+S and ?S) were transferred to a new Fe-free NS. Roots were collected 4 and 24 h from the beginning of Fe deprivation. Fe deprivation slightly increased root thiols content in both nutritive conditions (+S and ?S). ATP sulphurylase activity was enhanced by sulphur deprivation, but greatly depressed when Fe and S were both omitted from the nutrient solution. O-Acetylserine sulphydrylase activity was also enhanced by S deprivation; this activity was increased by Fe starvation in +S plants, while it was unaffected by Fe nutrition in ?S plants. S deprivation greatly increased uptake rates of 35SO4 2? (1.9 ± 0.1 vs. 5.2 ± 0.2 μmol g?1 root d.w. h?1); furthermore, Fe deficiency increased 35SO4 2? uptake rates by 11 and 55% in +S and ?S plants, respectively. Data show that Fe-deficiency in maize results in a higher ability to take up sulphate, while limiting the first step of S assimilation in S deprived plants. 相似文献
998.
KH Kjær SA Khan NJ Korsgaard J Wahr JL Bamber R Hurkmans M van den Broeke LH Timm KK Kjeldsen AA Bjørk NK Larsen LT Jørgensen A Færch-Jensen E Willerslev 《Science (New York, N.Y.)》2012,337(6094):569-573
Global warming is predicted to have a profound impact on the Greenland Ice Sheet and its contribution to global sea-level rise. Recent mass loss in the northwest of Greenland has been substantial. Using aerial photographs, we produced digital elevation models and extended the time record of recent observed marginal dynamic thinning back to the mid-1980s. We reveal two independent dynamic ice loss events on the northwestern Greenland Ice Sheet margin: from 1985 to 1993 and 2005 to 2010, which were separated by limited mass changes. Our results suggest that the ice mass changes in this sector were primarily caused by short-lived dynamic ice loss events rather than changes in the surface mass balance. This finding challenges predictions about the future response of the Greenland Ice Sheet to increasing global temperatures. 相似文献
999.
We present a report of dendritic ulcerative keratitis in a 4-year old locally immunosuppressed dog suspected to result from acute primary canine herpesvirus-1 (CHV-1) infection. The dog was presented for evaluation of mild blepharospasm and conjunctival hyperemia in the right eye (OD) shortly after attending a public boarding facility. For approximately 3 months, the dog had been receiving topical prednisolone acetate 1.0% and tacrolimus 0.02% in both eyes (OU) q12h for treatment of follicular conjunctivitis. Ophthalmic examination revealed three regions of corneal fluorescein retention OD. The lesions had a dendritic pattern, were approximately 2-3 mm in length, and were located at the dorsomedial, lateral, and ventromedial aspects of the cornea. No additional abnormalities were noted on complete ophthalmic and physical examinations. CHV-1 was identified in conjunctival samples OD by polymerase chain reaction, and paired CHV-1 serum virus neutralization antibody titers were positive and consistent with acute infection. Topical prednisolone acetate and tacrolimus were discontinued. The dog was treated with cidofovir 0.5% OU q12h for a period of 4 weeks, with resolution of corneal disease noted within 1 week of treatment. In conjunction with previous studies, this case report supports a central role for alterations in host immune status in the pathogenesis and clinical manifestations of CHV-1 ocular disease in dogs. 相似文献
1000.
Di Concetto S Mandsager RE Riebold TW Stieger-Vanegas SM Killos M 《Veterinary anaesthesia and analgesia》2012,39(1):99-105
Objective To investigate whether rostral extension of the hind limbs increases the cranio‐caudal dorsal interlaminar distance between the seventh lumbar vertebra and the sacral bone (LS distance) in sternally recumbent anesthetized dogs. Study design Prospective clinical study. Animals Eighteen dogs (eight neutered males, three intact males, six spayed females, one intact female) of various breeds, weighing 4–34 kg and ranging in age from 1 to 13 years. Methods Each dog was grouped by size: small (≤10 kg), medium (15–20 kg) or large (≥25 kg). Each dog was anesthetized and positioned in sternal recumbency. Computed tomography (CT) of the lumbosacral area was performed with the hind limbs resting on the stifle and the feet extended posteriorly, and then with the hind limbs extended rostrally. LS distance, craniocaudal dorsal interlaminar distance between sixth and seventh lumbar vertebra (L6–L7 distance), length of L7 vertebral body and lumbosacral angle (LS angle) were measured on a reconstructed mid‐sagittal CT image from the two hind limb positions. The measurements from the two hind limb positions for the whole dog population and by size were compared using Student’s T tests. Diagnostic interpretation of the CT images was performed. Results The length of L7 was taken as the reference value as it was not affected by hind limb position. LS distance, L6–L7 distance and LS angle were significantly higher when the hind limbs were extended rostrally in all three size groups. The CT images of ten dogs showed clinically undetected osteoarthrosis of the ileo‐ and lumbosacral area. Conclusions and clinical relevance Rostral extension of the hind limbs significantly increases LS and L6–L7 distance and LS angle even in dogs with clinically undetected osteoarthrosis of the ileo‐ and lumbosacral area, and may enhance the ease of lumbosacral epidural injection in sternally recumbent anesthetized dogs. 相似文献