缩宫素使用不当致母犬子宫破裂1例

缩宫素,又名催产素,临床主要用于催产、引产和产后子宫止血,以及治疗子宫积脓、促进子宫内容物的排出等.但缩宫素的使用方法很有讲究,如果使用不当可导致胎儿窒息死亡、子宫破裂等后果.我们在临床门诊上就遇到一起使用缩宫素不当导致的子宫破裂病例.     

达乌尔黄鼠染色体组型和G带分析

本文研究了达乌尔黄鼠东北亚种的骨髓细胞染色体组型和Ｇ－带带型。确定其染色体数为２ｎ＝３６，各染色体有其特有Ｇ－带带型。     

对动物防疫法中牵连违法行为的适用分析

1牵连违法情形在动物防疫法的存在动物卫生执法是《中华人民共和国动物防疫法》实现的重要途径和有力保障,严格遵守《中华人民共和国     

桑椹红色素稳定性的研究

研究了光和热等物理因素、常用食品添加剂、金属离子、氧化剂及还原剂对桑椹红色素稳定性的影响。结果表明 :pH值对桑椹色素的影响显著 ,在酸性条件下色素稳定 ;长时间高温和光照对色素有明显的破坏作用 ;金属离子Na+ 、Ca2 + 、Cu2 + 、Fe2 + 、Mg2 + 、Zn2 + 和Mn2 + 对桑椹色素色泽有一定的增色效果 ,但Fe3 + 则有明显的不良影响 ;食品中常用的葡萄糖、蔗糖、苯甲酸钠和山梨酸钾对桑椹色素无明显的不良影响 ;VC对桑椹色素有双重作用 ,而H2 O2 和Na2 SO3 对色素有严重的破坏作用。     

2007年第一季度饲料和原料中霉菌毒素调查报告

1样品概述 2007年1－3月,百奥明公司共收集来自全国各地的样本68份,其中：玉米28份,配合饲料样本34份,DDGS、豆粕、棉粕、菜粕、花生粕及玉米蛋白粉各1份。[第一段]     

Pharmacokinetics of mequindox and one of its major metabolites in chickens after intravenous, intramuscular and oral administration

Pharmacokinetics of mequindox and one of its major metabolites (M) was determined in chickens after intravenous (i.v.), intramuscular (i.m.) and oral administration of mequindox at a single dose of 10 (i.v. and i.m.) or 20 mg/kg b.w. (oral). Plasma concentration profiles were analyzed by a non-compartmental pharmacokinetic method. Following i.v., i.m. and oral administration, the areas under the plasma concentration-time curve (AUC(0-∞)) were 0.71±0.15, 0.67±0.21, 0.25±0.10 μg h/mL (mequindox) and 37.24±7.98, 36.40±9.16, 86.39±16.01 μg h/mL (M), respectively. The terminal elimination half-lives (t(1/2λz)) were determined to be 0.15±0.06, 0.21±0.09, 0.49±0.23 h (mequindox) and 5.36±0.86, 5.39±0.52, 5.22±0.35 h (M), respectively. The bioavailabilities (F) of mequindox were 89.4% and 16.6% for i.m. and oral administration. Steady-state distribution volume (V(ss)) of 1.20±0.34 L/kg and total body clearance (Cl(B)) of 13.57±2.16 L/kg h were determined for mequindox after i.v. dosing. After single i.m. and oral administration, peak plasma concentrations (C(max)) of 3.04±1.32, 0.36±0.13 μg/mL (mequindox) and 3.81±0.92, 5.99±1.16 μg/mL (M) were observed at t(max) of 0.08±0.02, 0.32±0.12 h (mequindox) and 0.66±0.19, 6.67±1.03 h (M), respectively. The results showed that mequindox was rapidly absorbed after i.m. or p.o. administration and most of mequindox was transformed to metabolites in chickens, with much higher C(max)s and AUCs of metabolite (M) than those of mequindox in plasma.     

昆明翠湖越冬红嘴鸥数量变化及投食量调查分析

昆明翠湖公园是红嘴鸥进城越冬数量最集中的地方,其数量分布在昆明水域内具有一定的代表性,翠湖公园是红嘴鸥的主要投食点,每年冬季,有大量市民和游客前来观鸥、喂鸥、护鸥。每年11月至次年3月翠湖公园的红嘴鸥数量存在规律性的变化,游客每天投放多少饲料量,要结合红嘴鸥的日食量和数量,受节假日的影响,游客数量变动大,投食量依赖于游客数量,经常出现喂鸥过量或不足的情况,翠湖水体补给有限,过量投食会带来富营养化污染,投食不足则不利于红嘴鸥的保护。通过对2009年11月至2010年3月期间昆明翠湖越冬红嘴鸥的数量进行分期统计,得出2010年翠湖越冬红嘴鸥前、中、后期的数量变化,参照红嘴鸥的能量代谢标准,分析计算昆明翠湖红嘴鸥饲料的合理投放量,为科学喂鸥提供依据,便于红嘴鸥和翠湖水体的保护。     

Pyruvate kinase is necessary for Brucella abortus full virulence in BALB/c mouse

Brucellosis, caused by a facultative intracellular pathogen Brucella, is one of the most prevalent zoonosis worldwide. Host infection relies on several uncanonical virulence factors. A recent research hotpot is the links between carbon metabolism and bacterial virulence. In this study, we found that a carbon metabolism-related pyruvate kinase (Pyk) encoded by pyk gene (locus tag BAB_RS24320) was associated with Brucella virulence. Determination of bacterial growth curves and resistance to environmental stress factors showed that Pyk plays an important role in B. abortus growth, especially under the conditions of nutrition deprivation, and resistance to oxidative stress. Additionally, cell infection assay showed that Pyk is necessary for B. abortus survival and evading fusion with lysosomes within RAW264.7 cells. Moreover, animal experiments exhibited that the Pyk deletion significantly reduced B. abortus virulence in a mouse infection model. Our results elucidated the role of the Pyk in B. abortus virulence and provided information for further investigation of Brucella virulence associated carbon metabolism.     

Development of a disperse dye immunochromatographic test for the detection of antibodies against infectious bursal disease virus

For investigating the feasibility of using disperse dyes as an immunoassay chromogenic marker, a disperse dye, DADISPERSE NAVY BLUE SP, was selected in analyzing antibody against infectious bursal disease virus (anti-IBDV). With the color intensity revealed in the disperse dye immunochromatographic test (DICT) strip as the objective function, the optimal dyeing conditions were found as follows: dye concentration absorbance (at lambda(max)=587nm)=3, pH 7, 50 degrees C, for 10min. Under these conditions, the resultant dyed-antibody (rabbit anti-chicken) can produce an optimal color intensity reading of 55,054 on the strip. For performing qualitative immunoassay, chicken sera samples taken from different farms were used for the anti-IBDV titre assessment. The results of DICT strips showed very high sensitivity and specificity as compared to that analyzed by FlockChek enzyme linked immunosorbent assay (F-ELISA) kits. For quantitative immunoassay, it was found that the color intensity measured with DICT was linearly correlated to that of F-ELISA titre (r(2)=0.9687). Therefore, DICT was further applied to the detection of chicken anti-IBDV sera under vaccination in the farms. The average titres of the sampling groups exhibited a strong agreement to that of F-ELISA. Accordingly, the DICT method developed in this study, shown to be reliable, cheap and simple in both qualitative and quantitative immunoassays, is particularly suitable for point-of-need testing (PONT) in agricultural applications.     

传染性法氏囊病病毒（IBDV）人工感染SPF鸡的病理学观察

取３２０只３０～４０Ｈ龄ＳＰＦ鸡，经滴鼻和点眼接种传染性法氏囊病病毒（ＩＢＤＶ），接种后２４～７２ｈ出现死亡，病死率５１．２５％，死亡鸡剖检变化主要为法氏囊肿胀、出血，甚至呈紫葡萄样，脾脏肿大、出血，骨骼肌出血，腺胃肌胃交界处出血。病理组织学变化，呈现以法氏囊淋巴滤泡内髓质淋巴细胞坏死为主的特征性病变，并可在巨噬细胞浆内发现病毒包涵体。电镜观察，在法氏囊内淋巴细胞、异染性细胞、巨噬细胞浆内，见大量晶格状排列的病毒粒子和包涵体，表明ＩＢＤＶ首先损害法氏囊淋巴滤泡髓质内未成熟的Ｂ淋巴细胞，病毒在淋巴细胞内以包涵体方式增殖。