High-level prokaryotic expression of envelope exterior of membrane protein of porcine epidemic diarrhea virus

The truncated fragment M' gene, encoding the exterior of the viral envelope protein of PEDV, was subcloned into prokaryotic expression vector pGEX-6p-1. The recombinant plasmid pGEX-6p-M' was constructed and transformed into E. coli BL21(DE3)pLysS for expression. SDS-PAGE analysis showed recombinant truncated M' protein was highly expressed by pGEX-6p-M' and the product fusion protein GST-M' reached 45% in the total bacteria proteins with the analysis of software AlphaImager2200. The preliminary purified recombinant protein was evaluated for its antigenicity and reactivity through Western blotting and indirect enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody against M protein of PEDV and porcine polyclonal anti-PEDV antiserum as the primary antibody. The results indicated the recombinant truncated M' protein should be candidate as a feasible recombinant diagnostic reagent.     

命题加工对态度的影响:基于APE模型

以APE模型为理论基础,采用实验法考察命题加工对外显、内隐态度的影响机制.实验一用直接呈现命题的干预方式发现短句描述材料命题学习使被试的外显态度发生显著改变,但并未以外显态度为中介导致内隐态度的改变;实验二用自我生成信息的干预方式发现自我生成不同性质信息使被试的外显态度发生显著改变,且以外显态度为中介改变了内隐态度.说明命题加工可以直接影响外显态度,且以外显态度为中介而对内隐态度产生影响,部分验证了APE模型的假设.     

小麦根腐病成株期品种抗性记载方法的探讨

小麦品种或株期对根腐病的抗病性,宜在小麦灌浆中期,调查记载平均病斑型、感病叶普遍率和平均严重率。应用品种平均严重率和高度抗病品种平均严重率的比值,参照5种不同抗病类型(HR,R、M、S、HS)标准对照品种的平均严重率比值范围,可确定品种的抗病或感病状况。上述参数可作为年度间品种抗病类型分析的依据。     

大白菜核基因互作雄性不育性的研究

通过大白菜雄性不育两用系间不育株与可育株杂交,育成了4个稳定遗传的大白菜雄性不育系。该不育系雄蕊深度退化,小孢子发育停滞于单核早期,雌蕊具有正常的结籽能力。遗传分析表明,该不育性是由细胞核内两对主效基因及一些微效基因共同控制的。提出了大白菜细胞核主效基因显性抑制及微效基因修饰核基因互作雄性不育性遗传模式和应用模式。     

东北大豆品种资源对大豆胞囊线虫病抗性的鉴定

1982～1986年,对从东北大豆产区收集的地方大豆品种202份,分别以大豆胞囊线虫的1号和3号生理小种进行田间和盆栽接种鉴定。播种后40～45天,调查每株根上的白色雌虫数,以Hill、丹豆4号和Lee作为感病对照品种,Peking作为抗病对照品种。鉴定结果:有4个小黑豆品种即磨石黑豆、北京黑豆、小粒黑豆和小粒黑,表现抗大豆胞囊线虫的1号和3号生理小种;有4个秣食豆品种表现抗3号小种,但不抗1号小种。     

Effect of Environment and Genetic Recombination on Subspecies and Economic Trait Differentiation in the F2 and F3 Generations from indicajaponica Hybridization

indica and japonica are the two most important subspecies of Asian cultivated rice. Identifying mechanisms responsible for population differentiation in these subspecies is important for indica-japonica hybridization breeding. In this study, subspecies and economic trait differentiation patterns were analyzed using morphological and molecular （InDel and Intron Length Polymorphism） data in F2 and F3 populations derived from indica-japonica hybridization. Populations were grown in Liaoning and Guangdong provinces, China, with F3 populations generated from F2 populations using bulk harvesting （BM） and single-seed descent methods （SSD）. Segregation distortion was detected in F3-BM populations, but not in F3- SSD or in F2 populations. Superior performance was observed with respect to economic traits in Liaoning compared with that in Guangdong and 1 000-grain weight （KW）, seed setting rate （SSR） and grain yield per plant （GYP） were significantly correlated with indica and japonica subspecies types. Analysis of molecular and morphological data demonstrated that the environment is the main factor giving rise to population differentiation in indica-japonica hybridization. In addition, we also found that KW, SSR and GYP are related to subspecies characteristics and kinship, which is possibly a significant factor resulting in economic trait differentiation and determining environmental adaptability. Our study has provided new insights into the process of population differentiation in these subspecies to inform indica-japonica hybridization breeding.     

油气管道可靠性评价指标及其计算

按照可靠性理论对管道工程某部分或整体的结构性质计算可靠性指标,然后根据这些指标评价其可靠性。用于管道工程可靠性评价的指标较多,欧美国家用故障率λ、可靠度R、可用度A;前苏联用可靠度H、准备系数K_r。列出了这些指标的计算公式,介绍了国外可靠性指标的计算资料。泵站由若干单件设备组合而成,其可靠性取决于单件设备的可靠性和它们的组合形式,据此可建立该系统可靠性模型,通常用可靠性逻辑框图表示。国外有关机构建立了可供油气管道可靠性评价用的数据库。     

西瓜G17AB雄性不育两用系选育

西瓜G17AB雄性不育两用系选育成功,为广泛利用西瓜杂一途径和简化杂一制种手续开辟了新的领域。G17AB系为一对隐性基因控制细胞核不育类型,育性分离稳定,自身农艺性状良好,配合力强,可直接配制杂一组合,亦可转育成新的不育材料。该两用系用于杂一制种,可提高杂交种纯度,节支省工,经济效益较大。     

一株猪源屎肠球菌的分离及其种属鉴定

为分离出可用作猪用微生态制剂的肠道益生菌株,本研究选择锦州市凌海大业乡生态猪场为试验采样点,采集10日龄左右健康仔猪肠道粪样12份。利用MRS琼脂培养基分离培养初筛目标分离菌株的形态特征和培养特性,再通过生理生化鉴定法和16SrRNA基因分子鉴定法,最终确定1株分离菌为屎肠球菌,并命名为LN/EF1312株,为后续肠道益生球菌的益生功能研究奠定了物质基础。