Association Mapping of Quantitative Trait Loci for Grain Size in Introgression Line Derived from Oryza Rufipogon

Grain size is one of the critical agronomic traits governing grain yield and quality in rice. However, the underlying genetic mechanisms that control grain size in rice are poorly understood. We used an introgression line derived from Zhonghui 8015 and Oryza rufipogon Griff. This introgression line was evaluated under two different environmental conditions to dissect the quantitative trait loci controlling grain size. Genome-wide association study (GWAS) was performed using 28 193 SNPs through a general linear model, and 56 significant SNPs on different loci associated with the 4 grain size traits were detected. Cloned genes including GS3 and qGL3 showed substantial effects on grain length and size. Seven new stable loci were identified with pleiotropic effects on grain size. Haplotype, gene expression analyses, combined gene-based associations, and functional annotations permitted the shortlisting of important dominant genes including GS3 and qGL3.     

Co-Ion Effect on Cr3+ Sorption by Amberlyst-15(H+)

Cr³⁺ sorption on strong acid exchanger Amberlyst-15(H⁺) is studied as a function of time and temperature using CrCl₃.6H₂O and [Cr₄(SO₄)₅(OH)₂] solutions. The rate is found to be governed by a mixed diffusion for both the solutions and faster for Cl^1? solution than SO₄ ^2?. The exchange capacities are found to be higher for Cl^1? system than SO₄ ^2?. From the rate constant values, the energies of activation are calculated using the well-known Arrhenius equation. Equilibrium data is explained with the help of the Langmuir equation. The Langmuir parameters are also found to be higher for exchange from the chloride solutions. Various thermodynamic parameters (??H^o, ??S^o, and ??G^o) for Cr³⁺ exchange on the resin are calculated. The ??G^o values are found to be negative while ??H^o and ??S^o are positive for both the Cr³⁺/Cl^1? and Cr³⁺/SO₄ ^2? systems. It is suggested that in case of Cl^1? solutions, the metal is exchanged as Cr³⁺, while in case of SO₄ ^2? solutions, the metal exchanging specie is CrSO₄ ⁺.     

Spatial Distribution and Temporal Trends of Polycyclic Aromatic Hydrocarbons (PAHs) in Sediments from Lake Maryut, Alexandria, Egypt

Surface and core sediments from Lake Maryut, Egypt, one of the most polluted lakes in Egypt, were analyzed for polycyclic aromatic hydrocarbons (PAHs) using gas chromatography?Cmass spectrometry. This investigation represents the first extensive study of the distribution and sources of PAHs in sediments from Lake Maryut. The total PAHs concentrations (sum of 39 PAH compounds) in surface sediments varied greatly depending on the sampling location and ranged from 106 to 57,800 ng/g dry weight with a mean concentration of 6,950 ng/g. The most polluted areas are distributed in areas which are mainly influenced by municipal sewage and industrial effluent discharges, suggesting a direct influence of these sources on the pollutant distribution patterns. PAH concentrations were one to two orders of magnitude higher in comparison with those reported for riverine/estuaries systems around the world. Molecular indices, such as pyrogenic index (PI), methylphenanthrenes to phenanthrene ratio, HMW_PAH/LMW_PAH, A-PAHs/P-PAHs, FL/FL?+?PY, BaP/BaP?+?C, IP/IP?+?BgP, and Per/??(penta-aromatics) were calculated to evaluate different hydrocarbon origins and their relative importance. In general, sediments from the main basin and northwest basin of Lake Maryut showed the highest PAH concentrations with petrogenic signatures, indicating major sources of petrogenic PAHs in the city. On the other hand, lower levels of PAHs with a pyrogenic signature were widely recorded in areas that are distant from anthropogenic sources. At other locations, both petrogenic and pyrogenic inputs were significant. The concentrations of perylene relative to the penta-aromatic isomers are dominant especially in locations associated with terrestrial inputs and in the deepest core sediments, indicating diagenetic origin for the presence of perylene. Temporal trends of PAH concentrations in both cores sediments were influenced by input pathways and followed the economic development and the environmental policies of the Egyptian Government in the last 15 years. Finally, PAH levels in sediments were compared with Sediments Quality Guidelines (effects range median?Ceffects range low) for evaluation probable toxic effects on organism. Results suggest an ecotoxicological risk for benthic organisms mainly in the main basin area, where high concentrations of PAHs were found in sediments influenced by anthropogenic activities.     

Effect of animal age,postmortem chilling rate,and aging time on meat quality attributes of water buffalo and humped cattle bulls

The study was aimed to investigate the influence of animal age, post‐slaughter chilling rate, and aging time on meat quality of M. longissimus dorsi (LD) of water buffalo (Bubalus bubalis) and humped cattle (Bos taurus indicus) bulls. After slaughtering, one side of carcasses was subjected to rapid chilling (RC) (0 ± 2°C) and other side was hanged in controlled room temperature (25 ± 2°C) for 3 hr, then allowed to the chiller (0 ± 2°C). The meat quality traits were analyzed at 1, 7, and 14 days of storage. It was noted that rapidly chilled carcasses from the younger animals of both species missed the ideal pH/temperature window, which affects the toughness of the meat. Buffalo meat presented higher shear force, color L* values, and lower b* value as compared to the cattle meat. Moreover, meat shear force values decreased while all color coordinates and cooking loss values increased with lengthening the storage time in both age groups of cattle and buffalo. In conclusion, the tenderness of cattle meat was superior to that of buffalo and RC adversely affect the shear force values of young cattle and both age groups of buffalo bulls.     

Production analysis of broiler farming in central Punjab,Pakistan

Tropical Animal Health and Production - This study examined the analysis of personal and production performance of broiler farming in 10 cities of central Punjab (Lahore, Sheikhupura, Kasur, Okara,...     

Boron nutrition for improving the quality of diverse canola cultivars

Abstract

Canola (Brassica napus L.), is the most important oilseed crop due to high oil contents and low concentration of erucic acid and glucosinolates. In Pakistan, oil seed production is not sufficient to fulfill the needs of the country. Thus, the planned experiment was aimed to evaluate the performance of different canola cultivars i.e. Faisal Canola, Pakola, PARC Canola hybrid and Rainbow at grown under various soil applied boron (B) levels viz., 0, 1, 2?kg ha^?1 under a rainfed environment. The experiment was laid out in randomized complete block design with four replications. Among the canola cultivars, the maximum seed oil contents were recorded in cultivar ‘Pakola’ whereas, higher linolenic acid and protein were recorded in cultivar ‘Faisal canola’ as compared to other cultivars. A synergistic effect was found between various levels of B and quality parameters of the canola seed; as higher concentration of oil contents were found when B was applied 2?kg ha^?1. Conversely the linolenic acid showed the antagonistic behavior with the various B levels. On the other hand, protein contents, oleic acid and erucic acid revealed non-significant differences under different B application rates. In conclusion, the cultivar ‘Pakola’ provided the highest oil content when the B was applied at 2?kg ha^?1; the low concentration of unsaturated fatty acid was observed in ‘PARC canola’ cultivar in the Pothwar region of Punjab, Pakistan.     

Yield and yield components of two irrigated red bean cultivars and some soil properties as influenced by wheat residues and nitrogen management

Crop residues are beneficial substances affecting crop production and soil properties. A field experiment was carried out to evaluate the effects of wheat (Triticum aestivum L.) residue rates (0, 25, 50 and 75%) combined with N levels (0, 34.5, 69, 103.5 kg ha^?1) on yield and yield components of two red common bean (Phaseolus vulgaris L.) cultivars and to monitor chemical soil parameters. The experiment was conducted at Research Center, College of Agriculture, Shiraz University, Shiraz, Iran for two years (2008–2009). The experiment was conducted as a split–split plot arranged in a randomized complete blocks design with three replications. The highest seed yield was obtained when 25–50% of residues were incorporated. The highest seed yield, seed weight per plant, 100-seed weight and seed number per pod were obtained with 103.5 kg N ha^?1 with no significant difference to 69 kg N ha^?1. Residue incorporation significantly increased soil organic carbon (SOC) as well as available K and P content. It is possible to sow red common bean as a double cropping by soil incorporation of 25–50% wheat residues with application of 69 kg N ha^?1.     

Exogenous glycinebetaine application improves yield under water-limited conditions in hybrid sunflower

Limited water availability is a severe threat to the sustainability of crop production. Exogenous application of glycinebetaine (GB) has been found very effective in reducing the adverse affects of water scarcity. This study was conducted to examine the role of exogenous GB application in improving the yield of hybrid sunflower (Helianthus annuus L.) under different irrigation regimes. There were three levels of irrigation: Control (four irrigations), three irrigations (irrigation missing at budding stage) and two irrigations (irrigation missing at budding and grain formation stage) in the experiment. While GB was applied exogenously at 100 mM GB each at budding and grain formation stages, the Control treatment did not receive any GB application. Data regarding yield, yield components and quality parameters showed that water stress reduced the head diameter, number of achene per head, 1000-achene weight and yield. Nonetheless, it was significantly improved by the exogenous GB application. Among the qualitative characteristics, protein contents were significantly increased by water stress at different growth stages but were reduced by exogenous GB application. Whilst oil contents were reduced by drought at different stages, GB application, however, did not ameliorate the negative effect of drought stress on achene oil contents. The effects of water stress and foliar application of GB were more pronounced when applied at vegetative stage than at the reproductive stage. Moreover, exogenous GB application was only advantageous under stress conditions.     

Soybean preference for Bradyrhizobium japonicum for nodulation

For examining the probability of increase in the occupation ratio of inoculated rhizobium in nodules, various Rj-soybean cultivars including the Rj ₂ Rj ₃ Rj ₄-lines of soybean were grown in a field of the Kyushu University Farm. Bradyrhizobium japonicum USDA110 that carries uptake hydrogenase (Hup⁺) was used as an inoculum. The relative efficiency of nitrogen fixation generally increased by the inoculation. However, there were no significant differences in the effects among the genotypes of the host plants. The occupation ratio of serogroup USDA110 in the nodules on the taproot of the inoculated plants was in the range of 77–100%, suggesting that the B. japonicum strain USDA110 infected taproots immediately after inoculation. The occupation ratios in the nodules on the lateral roots were 53–67, 40–86, 63–83, and 62–77% in inoculated plants of the non-Rj-, Rj ₂ Rj ₃-, Rj ₄-, and Rj ₂ Rj ₃ Rj ₄-genotypes, respectively, and they decreased in all the genotypes with the progression of growth. At the time of the first sampling, the occupation ratios on the lateral roots of these Rj ₂ Rj ₃ Rj ₄-genotypes showed values intermediate between those of IAC-2 (Rj ₂ Rj ₃) and Hill (Rj ₄) , which were the parent cultivars of the Rj ₂ Rj ₃ Rj ₄-lines, B340, B349, and C242. The reduction in the occupation ratio of the serogroup USDA110 for about 1 month after the first sampling was the lowest (0.13–0.16) in the Rj ₂ Rj ₃ Rj ₄-genotypes, excluding B349, followed by the non-Rj- and Rj ₂ Rj ₃-genotypes and highest (0.52–0.69) in the Rj ₄-genotypes, excluding Hill. Therefore, it was considered that the population of compatible rhizobia with host soybean plants increased in the rhizosphere with the progression of the development and growth. The results showed that with the expansion of the root area of host plants, the occupation ratio of type A rhizobia including the serogroup USDA110 was high. Therefore, the Rj ₂ Rj ₃ Rj ₄-genotypes were superior to other Rj-genotypes in terms of the inoculation effects of nodulation type A rhizobium, B. japonicum USDA110. However, the preference of the Rj ₂ Rj ₃ Rj ₄-genotype for serogroup USDA110 is not sufficient to rule out the competition with the other serogroups in this study. Therefore, the study should be centered on the isolation of more efficient (Hup⁺) and highly compatible rhizobial strains with the Rj ₂ Rj ₃ Rj ₄- genotypes.     

Sodicity Intensifies the Effect of Salinity on Grain Yield and Yield Components of Wheat

ABSTRACT

This study reports the relationship of the leaf ionic composition with the grain yield and yield components of wheat in response to salinity x sodicity and salinity alone. The study was conducted in soil culture in pots with three treatments including control (ECe 2.6 dS m^{? 1} and SAR 4.53), salinity (ECe 15 dS m^{? 1} and SAR 9.56), and salinity x sodicity (ECe 15 dS m^{? 1} and SAR 35). The soil was treated before being put in the pots and the pots were arranged in a completely randomized factorial arrangement with five replications. The seeds of three wheat genotypes were sown directly in the pots and the study was continued till the crop maturity. At booting stage, the leaf second to the flag leaf of each plant was collected and analyzed for sodium (Na⁺), potassium (K⁺), and chloride (Cl^?). At maturity, plants were harvested and data regarding grain yield and yield components were recorded. This study shows that salinity and sodicity in combination decreases the grain yield of wheat more than the salinity alone with a greater difference in the sensitive genotype. This study also shows that as for salinity, the maintenance of lower Na⁺ and higher K⁺ concentrations and higher K⁺: Na⁺ ratio in the leaves relates positively with the better development of different yield components and higher grain yield in saline sodic soil conditions. Although, the leaf Cl^? concentration was increased significantly by salinity as well as salinity x sodicity and would have affected the growth and yield, yet it does not seem to determine the genotypic tolerance or sensitivity to either salinity or salinity x sodicity.