Busulphan and thiourea as chemosterilants for the snail lymnaea acuminata

Treatment of the snail Lymnaea acuminata with an alkylating agent (busulphan) or a thiocarbamide (thiourea), for 3 days, significantly reduced the number of eggs and prevented embryonic development. Both the drugs caused a dose dependent reduction in the levels of protein, DNA, RNA and the activity of the alkaline phosphatase of the ovotestis, and a dose-dependent enhancement in the levels of amino-acids and the activity of the acid phosphatase. Discontinuation of the drug treatment for 7 days resulted in a near complete recovery in DNA and RNA levels, while there was no change in the levels of protein, amino-acids and the activities of the acid and alkaline phosphatases.     

Effect of field levelling quality on irrigation efficiency and crop yield

Proper land levelling in areas irrigated by canals is an essential pre-requisite for judicious use of scarce irrigation water. The concept of land levelling index, which is different from land uniformity coefficient used hitherto, has been adopted to evaluate quantitatively the precision or quality of land levelling work. Using this concept the quality of land levelling work being done in newly canal commanded areas has been assessed and it is demonstrated that the prevalent deficiency in levelling quality ranges from 21.4 to 42.2%. Field investigations have been carried out to assess quantitatively the effect of land levelling quality on irrigation and water-use efficiencies and crop yield. The study comprised five levelling indices viz., 1.2, 2.0, 2.5, 3.0 and 3.7 cm and two irrigation treatments, i.e., fixed depth and complete irrigation. The results show that irrigation application, distribution, and water-use efficiency are appreciably reduced with increase in levelling index or deterioration in levelling quality. The wheat grain yield is also similarly reduced and it is significantly less for levelling index values higher than 2.5 cm. The fixed irrigation depth treatment gives better results from the point of water economy than does the complete irrigation treatment. From this study, it can be inferred that to obtain reasonably high yield and judicious use of water at a reasonable cost the field levelling index should not have a value greater than 3.0 cm.     

Comparative study of cytotoxicity of Aeromonas spp. on four different cell lines

In vitro cytotoxicity is an important virulence property of motile mesophilic Aeromonas species. Cell-free supernatant prepared from 55 Aeromonas isolates including one A. hydrophila type strain (MTCC 646) were examined for their cytotoxic potential on four different cell lines (Vero, BHK-21, MDBK, B 95a). Results of the study revealed cytotoxic potential in 92.72% of the isolates. Analysis of data exposed significant variation among isolates in respect of their cytotoxicity. Vero cells proved to be most sensitive to aeromonal toxins and B 95a cells showed significantly (P<0.01) lower response compared to other cell lines. Sensitivities of BHK-21 and MDBK cell lines were in between Vero and B 95a.     

Endophytes from maize with plant growth promotion and biocontrol activity under drought stress

In the present study, 39 endophytic bacteria were isolated from different crops with main focus on maize roots and seeds. Endophytes were screened for drought stress tolerance, plant growth promoting (PGP) traits and antifungal activity. Out of 39 isolates, 32 could show drought tolerance up to–1.02 matric potential (MPa) and exhibited most of the plant growth promoting traits. But, only five isolates could show antagonistic activity against plant fungal pathogens. Based on the results, 10 promising isolates namely FTR, NFTR, FMZR9, FMZR2, MZ30V92, MRC12, MRC31, MRC33, MRC41 and MRR2 were selected and identified using biochemical and 16S rDNA gene sequencing as Pseudomonas aeruginosa (strains FTR and NFTR), Pseudomonas monteilii (strain FMZR2), Pseudomonas putida (strain FMZR9), Acitenobacter brumalii (strain MZ30V92), Enterobacter asburiae (strain MRC12), Sinorhizobium meliloti (strain MRC31), Pseudomonas thivervalensis (strain MRC33), Pseudomonas fulva (strain MRC41), and Pseudomonas lini (strain MRR2). Further, at–1.02 MPa all the 10 isolates showed PGP traits, and 3 isolates (FTR, NFTR and MRC12) showed antifungal activity. Thus, indicating that drought tolerant plant growth promoting antagonistic endophytic bacteria (PGPAE) helps in plant growth and disease management under drought stress.     

Effect of feeding some plant foods as source of dietary fibre on biological utilisation of diet in rats

Isoproteinous diets containing 1% cholesterol and 10% fibre derived from plant foods namely,peepalbanti (Ficus religiosa),barbanti (Ficus bengalensis),gullar (Ficus glomerata),teent (Capparis decidua) andkhejri beans (Prsopsis cineria) or pure cellulose, were fed to rats for 40 days. Effect of incorporation of these whole plant parts on feed intake, weight gain, feed efficiency ratio (FER), dry matter digestibility (DMD) and true protein digestibility (TPD) in weanling rats was studied. Their inclusion did not affect the weight gain significantly whereas all other parameters were influenced to a varying extent. Foods rich in lignin had relatively lower FER, DMD and TPD whereas cellulose and pectin rich foods had higher DMD and TPD.     

Energy Use and Output Assessment of Food-Forage Production Systems

An assessment of the energy requirements of different intensive forage production systems was carried out at the Indian Grassland Fodder, and Agroforestry Research Institute, Jhansi. This included assessment of energy use and output for five intensive crop production systems: (1) sorghum (single‐cut)–berseem, (2) cowpea–sorghum (single‐cut)–berseem + mustard–maize + cowpea, (3) sorghum (multi‐cut)–berseem + oats, (4) guar–oats–maize and (5) sorghum (single‐cut)–wheat–fallow. In all the systems, the Napier bajra hybrid (IGFRI‐3) was transplanted in regular plots of 50 m × 13 m. Results revealed that the total annual energy use was highest for sorghum (multi‐cut)–berseem + oats (36 606 MJ ha^?1), followed by sorghum (single‐cut)–berseem + mustard, sorghum (single‐cut)–berseem–cowpea, guar–oats–maize and sorghum (single‐cut)–wheat–fallow (for which values were 31 086, 30 449, 29 867 and 25 956 MJ energy ha^?1, respectively). The high value found for sorghum (multi‐cut)–berseem + oats might be attributable to the multi‐cuts in this system. Energy use by fertilizers represented the major part of the total energy use, amounting to 28–38 % in all treatments, followed by energy used in electricity, machinery, seeds, human labour and farmyard manure (FYM), in case of all with slight increase in input. In sorghum–wheat, energy use by seeds occupied the second position, followed by energy used in electricity, human labour, FYM and machinery/diesel. Pesticides contributed the least energy utilization in all the treatments. Herbicides were used for the control of weeds. Among the five forage production systems, sorghum (single‐cut)–berseem + mustard–maize + cowpea was found to be the most energy efficient, followed by sorghum (multi‐cut)–berseem + oats–sorghum (multi‐cut), sorghum (single‐cut)–berseem–cowpea, guar–oats–maize and sorghum (single‐cut)–wheat–fallow. Sorghum (single‐cut)–berseem + mustard–maize + cowpea increased the fertility of the soil, resulting in a higher percentage of organic carbon, higher availability of nitrogen and optimal balancing of the C:N ratio in the upper layers of the soil. These intensive crop production systems also maintain the optimum microbial population in the crop root zone. The benefit–cost ratio (B:C ratio) for the most energy‐efficient forage production system was 1.37 : 1. However, the highest B:C ratio was found in the sorghum–wheat rotation (1: 1.57).