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11.
T. D. Salla C. dos S. Silva K. L. de G. Machado L. V. Astarita E. R. Santarém 《林业研究》2018,29(3):623-629
Eucalyptus is very recalcitrant to in vitro culture. In this research, an efficient shoot organogenesis system was developed using 60-day-old plants of Eucalyptus globulus grown in vitro and non-aerated liquid medium to improve shoot proliferation. Cultures were initiated with hypocotyls and leaf segments from plantlets cultivated on semisolid ½ MS modified medium supplemented with 4.44 µM 6-Benzyladenine (BA) and 16.1 µM 1-Naphthaleneacetic acid (NAA). Calli were transferred to shoot induction medium, with either 0.5 or 2.7 µM NAA. Shoot multiplication was carried out on 4.44 µM BA + 0.5 µM NAA medium, and semisolid and non-aerated liquid systems were compared for improving shoot proliferation. Rooting of adventitious shoots was evaluated on medium containing NAA or Indole-3-butyric acid -IBA (5 and 16 µM). Callogenesis was obtained from both types of explants, although shoot formation was only obtained from leaf-derived calli. Shoot proliferation on 4.44 µM BA + 0.5 µM NAA resulted in the most shoots/callus. Non-aerated liquid medium was more efficient in promoting shoot multiplication (53.5 shoots/callus) than was semisolid medium (28.5 shoots/callus). Levels of phenolic compounds were significantly reduced in the shoots cultivated in liquid medium. Efficient rooting (76%) was obtained using 16 µM IBA. 相似文献
12.
Fagerholm Nora Eilola Salla Kisanga Danielson Arki Vesa Käyhkö Niina 《Landscape Ecology》2019,34(7):1769-1787
Landscape Ecology - Local scale assessments of ecosystem/landscape services in Africa are insufficient and lack relevance in landscape management. Also, few studies have explored the potential... 相似文献
13.
Detomidine and the combination of detomidine and MK‐467, a peripheral alpha‐2 adrenoceptor antagonist,as premedication in horses anaesthetized with isoflurane 下载免费PDF全文
Soile AE Pakkanen Marja R Raekallio Anna K Mykkänen Kati M Salla Annemarie de Vries Lauri Vuorilehto Mika Scheinin Outi M Vainio 《Veterinary anaesthesia and analgesia》2015,42(5):527-536
ObjectiveTo investigate MK-467 as part of premedication in horses anaesthetized with isoflurane.Study designExperimental, crossover study with a 14 day wash-out period.AnimalsSeven healthy horses.MethodsThe horses received either detomidine (20 μg kg−1 IV) and butorphanol (20 μg kg−1 IV) alone (DET) or with MK-467 (200 μg kg−1 IV; DET + MK) as premedication. Anaesthesia was induced with ketamine (2.2 mg kg−1) and midazolam (0.06 mg kg−1) IV and maintained with isoflurane. Heart rate (HR), mean arterial pressure (MAP), end-tidal isoflurane concentration, end-tidal carbon dioxide tension, central venous pressure, fraction of inspired oxygen (FiO2) and cardiac output were recorded. Blood samples were taken for blood gas analysis and to determine plasma drug concentrations. The cardiac index (CI), systemic vascular resistance (SVR), ratio of arterial oxygen tension to inspired oxygen (PaO2/FiO2) and tissue oxygen delivery (DO2) were calculated. Repeated measures anova was applied for HR, CI, MAP, SVR, lactate and blood gas variables. The Student's t-test was used for pairwise comparisons of drug concentrations, induction times and the amount of dobutamine administered. Significance was set at p < 0.05.ResultsThe induction time was shorter, reduction in MAP was detected, more dobutamine was given and HR and CI were higher after DET+MK, while SVR was higher with DET. Arterial oxygen tension and PaO2/FiO2 (40 minutes after induction), DO2 and venous partial pressure of oxygen (40 and 60 minutes after induction) were higher with DET+MK. Plasma detomidine concentrations were reduced in the group receiving MK-467. After DET+MK, the area under the plasma concentration time curve of butorphanol was smaller.Conclusions and clinical relevanceMK-467 enhances cardiac function and tissue oxygen delivery in horses sedated with detomidine before isoflurane anaesthesia. This finding could improve patient safety in the perioperative period. The dosage of MK-467 needs to be investigated to minimise the effect of MK-467 on MAP. 相似文献
14.
Rita Maria Alves de Moraes Tais Cristina Bastos Soares Lucinete Regina Colombo Maria Fernanda Spegiorin Salla Josie Gomes de Almeida Barros Newton Deniz Piovesan Everaldo Gonçalves de Barros Maurilio Alves Moreira 《Euphytica》2006,149(1-2):221-226
Summary The antinutritional factors found in soybean, lectin (SBL) and Kunitz trypsin inhibitor (SKTI), are usually inactivated by heat treatment. However, residual activity of these factors can be found in several types of soy-derived products. Heat treatment does not completely eliminate these factors, and in addition it may considerably decrease protein solubility. The genetic elimination of these antinutritional factors could be an alternative to the heat treatment. This study aimed to develop soybean lines devoid of SKTI and SBL in their seeds. The population under study was obtained by crossing the normal cv. Monarca with a soybean line lacking SKTI and SBL. Specific DNA primers were designed for the identification of the recessive alleles that condition the absence of SKTI and SBL. F2 seeds presenting the DNA markers that identify the recessive alleles were selected and the corresponding F2 plants were backcrossed with the recurrent parent (‘Monarca’), producing the BC1F1 generation. The F2 generation was analyzed by SDS-PAGE in order to confirm the genotypes of the F2 selected. The segregation tests confirmed that these traits are governed by two genes that segregate independently. 相似文献