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21.
家兔选育性状典型相关分析 总被引:3,自引:0,他引:3
选择日本大耳白和新西兰两个品种家兔的生长、繁殖、以及体尺3组共17个性状,应用多元统计方法进行了典型相关分析,结果表明;日本大耳白兔的初生重、初生窝重、21日龄重、泌乳力、胸围等5个性状,新西兰兔的产活仔数、初生重、21日龄重、泌乳力、断奶窝重、母兔体重等6个性状为主要性状。 相似文献
22.
JEV、PPV、PRRSV、PRV多联PCR的应用研究 总被引:3,自引:0,他引:3
用 JEV、PRRSV二联 PCR,PPV、PRV二联 PCR以及这 4种病毒 4联 PCR对来自内蒙、广州、广西、天津、北京、吉林病料进行检测 ,共检了 1 4 6份病料。其中 PRRSV阳性 7份 ,PPV阳性 1 2份 ,PRV阳性 2 1份 ,PRV和 PPV混合感染 4份。并对 5份人工接种 JEV小鼠病料检测 ,其中 4份为阳性。随后对部分阳性 PCR扩增产物进行点杂交和核苷酸测序鉴定 ,证实了 PCR扩增准确性。对内蒙 PRRSV阳性扩增带测序结果显示 ,我国流行 PRRSV为美洲型 ,在扩增片段的核苷酸序列上有 3个碱基差异 相似文献
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24.
采用百度试验法对云南松人工林木材进行了干燥特性的研究.结果表明:水煮后的木材干燥质量优于非处理木材,截面变形程度轻,且干燥时间缩短,并提出了云南松的干燥基准. 相似文献
25.
新疆各类陆栖脊椎动物近 70 0种 ,占中国这一类动物种数的 32 %,其中鸟兽资源尤其丰富 ,计有 42 5种鸟类和 1 5 5种兽类 (哺乳类 ) ,占中国鸟兽种数的 31 %。本文主要以新疆三大山区———阿尔泰山、天山、昆仑 -阿尔金山地区鸟类和哺乳类动物的多样性及区系分布作综合性介绍和分析。根据实地考察和以往文献资料 ,新疆阿尔泰山区记录在 1 2 4种鸟类 ,其中古北界北方型鸟类 5 1种 (占 41 .1 %) ,中亚型鸟类 31种 (占 2 5 .0 %) ,广布种鸟类 2 7种 (占 2 1 .8%) ,其他类型1 5种 (占 1 2 .1 %) ,显出以北方型泰加林鸟类为主的鸟类区系特征 ;分布的 5 0种兽类 ,区系上以古北界北方泰加林为主 ,即北方型兽类占该山区种数的 5 2 %。天山山区记录的鸟类大约2 81种 ,北方型种类占绝对优势 ,即北方型 1 0 6种 (占 37.72 %) ,北方广布型 76种 (占 2 7.0 4 %) ,中亚型 39种 (占 1 3.88%) ,高地型 1 8种 (占 6.41 %) ,广布型 33种 (占 1 1 .74%) ,东北型 9种 (占 3.2 1 %)。兽类分布有 62种 ,北方型兽类区系占优势 ,有 30种 (占 48.40 %) ,广布型 1 2种 (占 1 9.40 %) ,中亚型 1 1种 (占 1 7.70 %) ,特有种 9种 (占 1 4.5 0 %)。昆仑—阿尔金山地区 96种鸟类中 ,中亚类型成份占 2 8.1 %,高地型成分占 2 6%,北方型成? 相似文献
26.
CHENG Biao FU Xiao-bing SHENG Zhi-yong SUN Tong-zhu SUN Xiao-qing LI Jian-fu 《园艺学报》2002,18(11):1333-1336
AIM: To examine the expression of alpha-smooth muscle actin in scar tissue, and observe the phenomenon of apoptosis and its involvement in the process of pathological scarring and the presence of myofibroblasts or absence of cell in the dermis. To investigate the potential role of reparative cell apoptosis in hyperplastic scar formation. METHODS: The samples of scar were obtained from post-burn patients undergoing plastic operation in our burn unit recently, and the samples of control came from skin donor site of the same patient correspondingly. TUNEL assays were performed to evaluate the number of apoptotic cells in scar versus normal skin. In situ hybridization and immunohistochemistry staining technique were employed to determine the expression of different dermis cells markers in scar tissue and normal skin. RESULTS: There existed evident difference in apoptotic cells in the dermis between scars tissue and normal human skin. The expression positive cells were much more in hyperplastic scars than that in normal human skin; the apoptotic cells of proliferative stage were slight more than that of mature stage. However, in proliferative stage, the number of apoptotic cells was higher for the combination of hyperplastic scar than normally healed flat scars. But in mature stage, no obviously difference was detected between hyperplastic scar and normally healed flat scar. The monoclonal anti-α smooth muscle actin (ASMA) expression was significantly stronger in proliferative stage than that of mature stage. CONCLUSIONS: With reconstitution of dermal tissue, myofibroblasts containing alpha-SM actin disappear under normal wound healing, probably as a result of apoptosis. The myofibroblast play a critical role in wound closure and in the pathologic sequelae of healing. 相似文献
27.
AIM and METHODS: Total RNA was extracted from 6th rat subcultured pulmonary artery smooth muscle cells(PASMC) exposed to continual chronic hypoxia or normoxia and the effects of chronic hypoxia on the changes of Kv1.3,Kv2.1,Kv3.1 mRNA in cultured PASMC induced by acute hypoxia were studied by semiquantitative RT-PCR in vitro. RESULTS:①Kv1.3,Kv2.1,Kv3.1 genes were found to be expressed in PASMC of rats exposed either to hypoxia or normxia.②The expression of Kv2.1 and Kv3.1 in 6th subcultured of PASMC in normaxia group could be upregulated by exposure to acute hypoxia,the levels of Kv2.1 and Kv3.1 mRNA were significantly increased from 0.646±0.092, 0.782±0.104 to 1.059±0.134, 0.985±0.116,respectively (P<0.01,n=5). ③PASMC cultured continuously in chronic hypoxia for 6 subcultures and then exposed to normoxia for 12 h,thereafter the expression of Kv2.1 and Kv3.1 were downregulated by acute hypoxia for 6 hours.The level of Kv2.1 mRNA was significantly decreased from 1.008±0.117 to 0.649±0.097 (P<0.01,n=5). CONCLUSION:Kv2.1,Kv3.1 genes might be oxygen sensitive genes.Chronic hypoxia might change the response of these Kv genes of PASMC to acute hypoxia and down-regulate its expression,which might probably decrease the role of Kv in HPV. 相似文献
28.
AIM: To investigate the relationship between somatostatin and the pathologic type, estrogen receptor,DNA ploidy of nuclei in tumor cells of breast cancer.METHODS: 67 cases of primary breast cancer and 25 cases of benign breast tumor were examined by immunohistochemical stretomyces avidin peroxidase method. 26 cases of breast cancer selected at random were analyzed by flow cytometry.RESULTS: Somatostatin expressed significantly higher in low malignant breast cancer than that in high malignant breast cancer (P<0.05). Most of cancers with positive staining of somatostatin were diploidy,most of cancers with negative staining were aneuploidy,there had significant difference between two groups (P<0.05).CONCLUSION: Somatostatin may delay the progress of breast cancer,and somatostatin levels in cancer tissues may become a useful indicator for assessing prognosis of patients with breast cancer. 相似文献
29.
WANG Li-wei CHEN Li-xin MAO Jian-wen ZHU Lin-yan NIE Si-huai ZHONG Ping SUN Xue-rong CAI Bo LI Pan 《园艺学报》2004,20(8):1349-1352
AIM: To investigate the relationship between osmolarity, cell volume and cell proliferation in nasopharyngeal carcinoma cells. METHODS: MTT method was applied to detect the proliferation ability of the poorly-differentiated nasopharyngeal carcinoma cell (CNE-2Z) under various osmolarity conditions. The flow cytometry was used to analyse cell cycle distribution. Cell volume was obtained by the image analysis of living cells and cell viability was determined by the trypan blue assay. RESULTS: Cultivation of cells under the hypertonic conditions of 370 and 440 mOsmol/L increased cell volume by 8.7% and 27.8% and facilitated cell proliferation by 22.2% and 33.9%, respectively. However, hypotonic incubation of cells with osmolarity of 160 and 230 mOsmol/L decreased cell volume by 12.8% and 4.1% and inhibited cell proliferation by 34.0% and 15.6%, respectively. Cell volume was positively correlated with cell proliferation rate. Long-term cultivation of cells under anisotonic conditions did not significantly alter cell cycle distribution, but hypotonic cultivation decreased cell viability. CONCLUSION: Proliferation of nasopharyngeal carcinoma cells was closely correlated with the osmolarity of culture medium and cell volume. Hypotonic cultivation may inhibit cell proliferation by decreasing cell volume to facilitate cell death mechanisms. 相似文献
30.
AIM: To further investigate the role of PKARⅠβ in the growth-promoting effects of shuang long Jiegu pill (SLJGP), a Chinese medicine, on cultured osteoblasts. METHODS: pcDNA- antiPKARⅠβ, a recombinant expressing the antisense sequence of PKARⅠβ, was constructed and transformed HFOB1.19 by lipofectin. MTT was undertaken to assess the cell growth with the treatment of high dosage of SLJGP containing serum. RESULTS: Antisense gene blocked the growth-promoting effects of SLJGP containing serum on HFOB1.19. CONCLUSION: The function of SLJGP is closely related to cAMP-dependent protein kinase A. 相似文献