Prevention of isinglass on the chronic atrophic gastritis in rats and its mechanism

AIM: To evaluate the effect of isinglass on chronic atrophic gastritis(CAG) in rats and its mechanism. METHODS: An animal model of CAG in accordance with the previous experience of combined administration of 60% ethanol, 20 mmol/L sodium deoxycholate and 0.1% ammonia water was established in SD rats. Isinglass was used as preventive therapy while we were establishing CAG rat model. Finally all the rats were executed and pathologic changes of the gastric mucosa were studied by gross appearance and microscopy and serum epidermal growth factor (EFG) and growth hormone(GH) contents were tested. RESULTS: In each isinglass prevention group, inflammation grade of gastric antrum was less than that in model group (P<0.01) while the mean ratio of the thickness of gastric mucosal gland and muscularis mucosa (L1/L2), the number of gastric glands in 1 mm lengths of mucosal layer in longitudinal sections were much better than those in model group (P<0.01).They were very close to normal control group (P>0.05). The expression of proliferating cell nuclear antigen (PCNA) in gastric mucosa and serum EFG level were higher than those in model group (P<0.01, P<0.05), but serum GH content showed no different between isinglass prevention group and model group. CONCLUSION: Isinglass preventes the gastric mucosal atrophy in the CAG model. Its mechanism may be related to the effects of decreasing the gastric mucosal damage， promoting the cell proliferation and increasing of internal EFG secretion.     

Effect of rhEGF activating extracellular signal-regulated kinase on the expression of Bcl-2 and p53 protein in human amniotic cells

AIM: To explore the effect of recombinant human epidermal growth factor (rhEGF) on the ERK1/2 pathway and its safety in use when the proliferation of cultured FL cells (human amnion epithelial cells) were promoted by rhEGF.METHODS: FL cells were stimulated by rhEGF at various concentrations.The proliferation rate of FL cells was evaluated by MTT assay.Western blotting was used to detect phosphorylation of ERK1/2 (p-ERK1/2) and the change of Bcl-2 and P53 protein.RESULTS: The proliferation rate FL cells reached the maximum when the concentration of rhEGF was 10 μg/L (42.4％，P<0.01).Western blotting showed that the activity of p-ERK1/2 increased significantly when rhEGF ranged in 10 μg/L to 60 μg/L.Level of Bcl-2 was unchanged in each groups.Level of P53 decreased significantly when the concentration of rhEGF was 60 μg/L.CONCLUSION: It is safe when rhEGF presents in optimization concentration of 10 μg/L.The capability of apoptosis may be depressed when rhEGF is in larger dose.     

浅探《思想道德修养与法律基础课》的实践教学方法

切实提高“思想道德修养与法律基础课”课教学的实效性，是当前”思想道德修养与法律基础课”课教学方法改革的重要课题。如何在教学方法上突破传统单一的理论灌输方法模式，创新更多的教学方法，才能有效改进大学生思想政治教育工作。     

聆听蔬菜种业声音 鉴赏蔬菜新优品种——第十一届广东种业博览会纪实

2012 年12 月12 日，第十一届广东种业博览会在广州成功举办，精彩求真的种业论坛、国内外蔬菜种子公司的群英荟萃、上千个新优品种的田间展示为2012 年的蔬菜种业奉献了一场丰盛的年终大餐。     

HPLC法测定蒺参胶囊中人参皂苷Re的含量

目的建立蒺参胶囊中人参皂苷Re的含量测定方法。方法采用高效液相色谱法测定。结果人参皂苷Re在1.65～6.05μg之间与峰面积呈良好的线性关系(r=0.999 7),平均回收率为95.98%,RSD为1.35%(n=5)。结论方法简便、可靠、准确,可用于蒺参胶囊的质量控制。     

猪瘟乳前免疫及其抗体检测

本试验对新生仔猪哺乳前初次进行猪瘟疫苗免疫，至７０日龄时进行第二次免疫，免疫持续至７０日龄和１５０日龄时两次应用Ｄｏｔ－ＥＬＩＳＡ方法进行检测，结果表明，阳性率分别为９２．３％和９１．２５％，说明此免疫方法是一较好的预防猪瘟的免疫程序。     

塞罕坝地区湿地资源及其保护对策

通过对塞罕坝地区范围内湿地资源的调查、汇总和分析,结果表明:此区湿地类型众多,面积达5088.5hm2;湿地植物种类比较丰富,有48种之多;以湿地为生境的鸟类有50多种。说明了湿地资源的丰富性、多样性及重要性,并对其存在的问题及解决方法进行了初步的探索。     

核桃楸树皮的没食子酸、鞣花酸及水解单宁成分研究

研究了核桃楸树皮丙酮-水提取物的水溶性萃取部位的化学成分.采用Sephadex LH-20柱色谱及薄层色谱等方法进行分离,经波谱分析及理化性质测定,鉴定了6个化合物:没食子酸(1)、鞣花酸(2)、1,2,6-三没食子酰葡萄糖(3)、1,3,6-三没食子酰葡萄糖(4)、1,2,4,6-四没食子酰葡萄糖(5)及1,2,3,4,6-五没食子酰葡萄糖(6).化合物2、4、6为首次从该植物中分得.     

银中杨生长特性的研究

通过对银中杨生长特性研究表明 ,银中杨在生长方面超过当地推广树种小黑杨 ,其中材积生长超过小黑杨 73.4 %～ 2 6 9.2 % ;在本地区生长超过目前东北、内蒙及华北地区的优良杨树品种。探讨了银中杨生长规律及适宜栽植密度 ,并对银中杨生长划分 5个时期 ,为合理经营银中杨提供科学依据。     

杉木人工林地力衰退研究概述

介绍了我国杉木人工林地力衰退的现状，从森林土壤条件、杉木人工林的营养循环特点和人为经营措施等方面分析了导致杉木人工林地力衰退的原因，提出营建人工复层林、林地施肥、适当延长轮伐期，加强植被管理和进行定位观测等措施防止地力衰退。