毛竹种子内生真菌的分离与分子鉴定

从毛竹种子中分离得到不同形态特征的内生真菌7株,通过菌落形态特征、r DNA-ITS片段的扩增、克隆、序列测定和生物信息学分析等手段,对其中的5个菌株进行鉴定。确认2号菌株为枝孢属枝状枝孢,是一种重要的致霉菌。4号和7号菌株属于刺盘孢属,能引起竹炭疽病,是种常见的竹杆部病害。5号菌株为竹黄属竹黄菌,能使竹子患赤团子病,但竹黄具有重要的药用价值。虽然2号与6号菌株菌落形态不尽相同,但鉴定为同一种菌。     

榄溪菊韵的景观特色分析

小榄菊花会是广东省中山市小榄镇一项重要的民俗活动。以菊花会200周年景点之一的榄溪菊韵为例,从园林景观的角度分析了菊花与湿地景观融合、菊花与园林造景等方面的关系,为菊花在园林中的应用提供了新的思路,阐述弘扬小榄菊花文化和保护湿地的重要性。     

基于粒子群算法的海洋平台热介质锅炉PID参数优化

文中采用串级控制作为海洋平台热介质锅炉的控制方案，采用粒子群算法对PID控制器的比例带啄、积分时间Ti进行最优化，从而实现热介质锅炉温度的自动控制。通过粒子群算法进行串级控制系统PID参数优化，提高了控制系统的控制精度、可靠性、稳定性，具有工业应用价值。     

红松无性系种子园与实生种子园种实性状观测

建立红松无性系种子园和实生种子园的实践证明:嫁接母树的结实株数、结实率、公顷产种量、种子千粒重等9项种实性状均优于实生母树,关键种实性状指标结实率、公顷产种量、出种率、种子千粒重、出仁率分别是实生母树的3.67倍、2.74倍、1.16倍、1.02倍和1.12倍.     

转录组的测序方法及应用研究概述

转录组研究是基因的结构和功能研究的基础,转录组测序技术在近年来取得了很大的进展,被应用到多种动植物中。利用其能够获得大量未知序列的优势,已经被广泛应用到未知基因挖掘、分子调控及相关代谢途径中。该研究主要介绍了转录组研究的方法、转录组测序技术的发展及其在动植物中的重要应用,并讨论其存在的问题及发展趋势。     

BI-2000图像分析系统在家兔失血性休克实验中的应用

家兔失血性休克实验是《医学机能学》实验中的一个重要组成部分,在日常教学过程中,传统经典实验只观察血压的变化,针对这一问题,我院引进BI-2000图像分析系统,并在2014级临床医学教改班家兔失血性休克实验中应用,使学生对于休克发生、进展的机制有了更深刻的理解,对休克各期微循环血液灌流的变化特点有了更直观的认识。     

Nutrient uptake requirements with increasing grain yield for rice in China

Improved estimates of nutrient requirements for rice（Oryza sativa L.）in China are essential to optimize fertilization regulation for increasing grain yields and reducing the potential of environmental negative influences,especially under high-yielding intensive systems.A database involving rice grain yields,nutrient concentrations and accumulations collected from on-field station experiments in the literatures published from 2000 to 2013 in China was developed to understand the relationships between grain yields and plant nutrient uptakes,and to quantify nutrient requirements for different yield levels.Considering all data sets,rice grain yield ranged from 1.4 to 15.2t ha^-1with the mean value of 7.84t ha^-1,and ca.10.4%of yield observations were higher than the yield barrier level of 10 t ha^-1.N requirement to produce one ton grain was 21.10 kg for the yield range<4.0t ha^-1with a high variation of 45.8%.Except of the yield range<4.0t ha^-1,the values of N requirement,firstly increased from 18.78 kg for yield range 4.0-5.5 t ha^-1to 20.62 kg for yield range 7.0-8.5 t ha^-1,then decreased slightly to 19.67 and19.17 kg for the yield range 8.5-10 and>10 t ha^-1,respectively.Phosphorus（P）and potassium（K）requirements showed increasing trends,from 3.51 and 19.87 kg per t grain for<4.0 t ha^-1yield range to 4.10 and 21.70 kg for>10.0t ha^-1range.In conclusion,nutrient requirement varied with increasement of grain yield,and N,P and K presented various response trends,increasing,declining or stagnating,which would be of great benefit for improving fertilizer strategies.     

生殖细胞早期发生与减数分裂启动调节机制的研究进展

卵巢性生殖疾病发生的主要原因之一是卵子发生过程中减数分裂发生或染色体分离异常。为了更好地进行这些生殖疾病的诊断和治疗,需要充分地了解个体发育的生物学过程中及关键时间点。鉴于此,本文主要阐述了哺乳动物生殖细胞早期发生和减数分裂启动的生理过程,介绍生殖细胞的出现、迁移、性别分化和减数分裂的整个过程。深入了解卵母细胞发生过程将为利用体外发育来源的卵母细胞治疗卵巢性生殖疾病,克服女性不孕、卵巢早衰等重大疾病提供一定的理论支持。     

Cloning and Sequence Analysis of Complete Genome of Porcine Circovirus Type 2 from Liaoning Province

It was aimed to lay a foundation for epidemiological survey of porcine circovirus type 2(PCV2) in Liaoning province and selection of highly homologous strain inactivated vaccine.Some pigs suspected of having postweaning multisystemic wasting syndrome (PMWS) from Shenyang,Chaoyang,Tieling,Dalian and other places in Liaoning province,were detected by PCR method,then the whole genome of 10 positive samples were cloned and sequenced.The result showed that all of them were virulent strains,9 strains were 1767 bp and 1 strain was 1768 bp,and there was a T base additon at 1039 bp.The nucleotide homology was 95.1% to 98.5% with HM038034 and JQ692110.In the phylogenetic tree,JHZ2 was PCV2a type,the other 9 strains were PCV2b type,DLS38,LYD32 and SYX7 were in the same branch and same onset time.The amino acid homology of ORF2 was 89.6% to 100.0%,there were 35 mutation points,large degree of variation,the only glycosylation site (NYS) was conserved.10 strains did not change significantly,mutants were not due to PCVD,PCV2b was the predominant type,and PCV2 was related with seasonality.     

水稻卷叶突变体rlm1的基因克隆

叶片是植物光合作用的主要场所,优良的叶片形态有利于塑造理想的株型,提高光合效率。为了研究叶片形态建成的分子机制,自水稻T-DNA插入突变体库中筛选获得1个叶片半卷曲的卷叶突变体(roll leaf mutant,命名为rlm1),突变体rlm1主要特征为成熟叶片沿中脉向内卷,叶片最终卷成直立半圆筒状,叶片卷曲度达0.64,叶片直立参数达95,且光合效率显著优于野生型。通过图位克隆技术,确定突变体rlm1突变位点位于LOC_Os03g06654基因的第1个内含子,LOC_Os03g06654基因编码黄素单氧化酶(flavin-containing monooxygenase),RT-PCR表达分析表明LOC_Os03g06654基因因T-DNA插入而导致完全失活。该基因与已报道的水稻卷叶基因Os COW1(Constitutively Wilted 1)为等位基因,而且突变体rlm1所表现的农艺性状均佳,可期待利用该突变体进行高光合的育种实践。