福州地区露地越冬快菜品种比较试验

为筛选出适合福州地区露地越冬栽培的耐抽薹快菜品种,以万农5号为对照品种,对来自全国各地的20个快菜品种进行了农艺性状、耐抽薹性和产量比较的研究试验。结果表明,早熟和谐5号具有整齐度高、叶色绿、心叶色黄、耐抽薹性强等特点,每667 m~2产量为3 173.4 kg,比对照增产22.86%。说明早熟和谐5号适合在福州地区露地越冬茬口推广种植。     

苹果赤霉素信号转导因子MdGAMYB的克隆和表达分析

以‘长富2号’苹果为试验材料,从其短枝顶芽中克隆得到1个赤霉素信号转导因子MdGAMYB,对其进行生物信息学和表达分析。结果表明,MdGAMYB的开放阅读框(ORF)长度为1 656bp,编码551个氨基酸,蛋白质分子量为59.741 kD。生物信息学分析表明MdGAMYB编码的蛋白存在多个糖基化位点和磷酸化位点;序列分析表明,Md GAYMB和其他物种的GAMYB蛋白有很高的相似性,均含有保守的R2R3 DNA结合域和GAMYB家族所特有的Box1,Box2和Box3保守区域;系统进化分析表明,Md GAYMB与梨、梅花、草莓、枣和葡萄等的GAMYB蛋白具有较高的同源性。实时荧光定量PCR分析表明,Md GAYMB具有组织表达特异性,在叶片、花和芽中的表达量较高。外源GA3处理抑制了花芽孕育和翌年成花,抑制MdGAMYB的表达。在易成花品种‘烟富6号’中的表达量高于难成花品种‘长富2号’。     

干旱胁迫下外源油菜素内酯对玉米幼苗光合作用和D1蛋白的调控效应

为了揭示干旱胁迫下外源油菜素内酯对玉米幼苗光合作用的保护机制,采用溶液培养的方法,以驻玉309为试验材料,研究外源油菜素内酯(BR)预处理及20% PEG-6000模拟干旱胁迫后玉米幼苗的生长参数、叶绿素含量、光合参数、叶绿素荧光参数及D1蛋白含量的变化。结果表明,与干旱胁迫处理(PEG)相比,BR+PEG处理的玉米苗株高增加45.87%,根长增加20.56%,总干物质积累增加8.01%,叶片相对含水量提高4.50%,叶绿素a含量增加26.32%,光合参数(Pn、Gs、Ci、Tr)分别提高9.57%,38.23%,30.19%,28.12%,光合系统Ⅱ(ΦPSⅡ)活性提高了20.48%,最大光化学效率提高了0.66%,光合系统Ⅱ绝对电子传递速率(ETR(Ⅱ)和相对电子传递速率r ETR(Ⅱ))分别提高20.40%和31.02%;D1蛋白含量增加37.34%(P0.05)。说明在干旱胁迫条件下叶片喷施BR可以改善玉米幼苗的生长发育,减缓光合系统的损伤,促进D1蛋白质的稳定,从而提高玉米幼苗对干旱胁迫的适应性。     

吉林延龄草种子内源抑制物质初步研究

为探讨吉林延龄草种子内源抑制物质生理活性及种子中抑制物质的去除方法,采用乙酸乙酯、甲醇、水提取吉林延龄草种子并测定其提取物对白菜、水稻幼苗生长的抑制活性,用温水(25,35,45℃)浸种并测定各批次浸提液抑制活性,结果表明,吉林延龄草种子浸提液(乙酸乙酯提取液除外)对白菜幼根及胚轴,水稻幼根均有显著抑制作用,且抑制物质的活性随着提取物浓度的增加而增强,吉林延龄草种子水提取液(C)对白菜和水稻幼苗生长的抑制活性高于甲醇提取液(B)的生物活性,吉林延龄草种子乙酸乙酯提取液对白菜和水稻幼苗无显著抑制作用(p＞0.05),吉林延龄草种子经乙酸乙酯、甲醇溶液提取后,再经蒸馏水提取获得的水提液Ⅱ(E)对白菜和水稻幼苗生长的抑制活性减弱;温水浸泡可将其内源抑制物质浸提出来,且随着浸种时间的增加,浸提液对白菜幼根及胚轴的抑制作用呈现先增加而后逐渐降低的趋势.25℃蒸馏水浸泡种子第5天浸提液抑制作用达到最大,对白菜幼根及胚轴的抑制作用分别达到65.89％和25.69％,35℃蒸馏水浸泡种子第3天浸提液抑制作用达到最大,对白菜幼根及胚轴的抑制作用分别达到82.14％和59.93％,45℃蒸馏水浸泡种子第2天浸提液抑制作用达到最大,对白菜幼根及胚轴的抑制作用分别达到90.62％和83.86％.综合分析表明,吉林延龄草种子中存在活性较高的内源抑制物,用45℃温水浸泡可有效除去种子内源抑制物质.     

生长年限对苜蓿和柠条光合特征及土壤水分的影响

为了探究生长年限对衰败期苜蓿和中老龄柠条的叶片光合生理特征及土壤水分的影响,在2014年于地处黄土高原水蚀风蚀交错带强烈侵蚀中心的神木六道沟小流域,测定了不同生长年限苜蓿（ALF₁₀、ALF₁₃、ALF₃₃和ALF₄₉）和柠条（KOP₁₀、KOP₂₅、KOP₄₃和KOP₇₃）的叶片光合参数、叶结构性状参数以及0~400 cm土层土壤体积含水量（SWC_0-400）。结果表明：对于衰败期苜蓿,ALF₁₀和ALF₁₃叶片净光合速率（P_n）差异不显著（P>0.05）,而后随生长年限的延长逐渐降低,ALF₃₃和ALF₄₉比ALF₁₃叶片的P_n（24.01 μmol·m^-2·s^-1）分别降低了2.32 μmol·m^-2·s^-1和7.76 μmol·m^-2·s^-1。相比ALF₁₀,随生长年限延长SWC_0-400恢复至10.88%（ALF₁₃）,而后随着生长年限延长降低至8.81%（ALF₃₃）和6.12%（ALF₄₉）。土壤水分对ALF₃₃的限制作用不明显,水分胁迫使ALF₄₉非气孔限制值比ALF₃₃增大了0.340。对于中老龄期柠条,叶片P_n随生长年限的延长先升高后降低,KOP₂₅和KOP₄₃叶片P_n最大且两者差异不显著（P>0.05）,比KOP₁₀（6.62 μmol·m^-2·s^-1）分别增大了6.57 μmol·m^-2·s^-1和7.66 μmol·m^-2·s^-1,KOP₇₃比KOP₄₃叶片P_n降低了4.95 μmol·m^-2·s^-1。对于同为黄绵土生长的KOP₁₀、KOP₄₃和KOP₇₃,SWC_0-400随生长年限延长逐渐上升,分别为8.50%、9.06%和10.71%。土壤水分恢复使KOP₄₃叶片气孔及非气孔限制值比KOP₁₀柠条降低了0.185和2.180。此外,柠条叶片相对叶绿素含量（SPAD）与P_n呈极显著正相关（P<0.001）,相关系数为0.514。研究结果表明衰败期苜蓿和中老龄期柠条叶片光合性能呈波动式变化,这与土壤水分的相互作用有关。     

miR-140-3p inhibits viability,invasion and migration of non-small-cell lung cancer A549 cells by targeting PD-L1

AIM: To explore the target relationship between microRNA-140-3p (miR-140-3p) and programmed cell death ligand 1 (PD-L1) and their effect on the viability, migration and invasion of non-small-cell lung cancer A549 cells.METHODS: RT-qPCR was used to detect the miR-140-3p expression in HLF-1, A549 and H1299 cells, and then the A549 cells with the most significant difference were selected as the subsequent research object. TargetScan software and dual-luciferase reporter assay were performed to predict and confirm the target relationship between miR-140-3p and PD-L1. RT-qPCR and Western blot were used to determine the effects of miR-140-3p mimic and inhibitor on PD-L1 expression level. MTT assay was used to detect the viability of A549 cells. Transwell assay was performed to detect the migration and invasion abilities of the A549 cells.RESULTS: miR-140-3p was significantly down-regulated in the A549 cells and H1299 cells (P<0.05). Transfection with miR-140-3p mimic decreased the expression of PD-L1 and inhibited the viability, migration and invasion of the A549 cells. Transfection with pcDNA3.0-PD-L1 reversed the inhibitory effect of miR-140-3p on the viability, migration and invasion of the A549 cells.CONCLUSION: miR-140-3p inhibits the viability, migration and invasion of A549 cells by targeting PD-L1.     

MicroRNA-145 inhibits epithelial-mesenchymal transition in human renal cancer A-498 cells by ADAM28

AIM: To investigate the inhibitory effect of microRNA-145 (miR-145) on epithelial-mesenchymal transition (EMT) in renal cancer A-498 cells. METHODS: The A-498 cells were transfected with miR-145 mimics (M145) and mimic negative control(MNC), which served as M145 group and MNC group, respectively. Mock control (MC) group was set up using untreated A-498 cells. The expression level of miR-145 in each group was detected by RT-qPCR. Transwell assay was used to detect the invasion ability of the cells. The protein expression of vimentin, E-cadherin and ADAM28 was determined by Western blot. Bioinformatic method was used to predict the target genes of miR-145. Antagonistic effect of ADAM28 over-expression on the inhibition of EMT by miR-145 was detected by Western blot. The relationship between miR-145 and ADAM28 was analyzed by dual-luciferase reporter assay. RESULTS: The expression level of miR-145 in M145 group was significantly up-regulated than that in MC group (P<0.05). The number of invasive cells in M145 group was 12.78±3.37, which was significantly lower than that in MC group (P<0.05). ADAM28 may be the target gene of miR-145. Compared with MC group, the protein expression of vimentin and ADAM28 in M145 group was significantly decreased (P<0.05), while the protein expression of E-cadherin was significantly increased (P<0.05).After ADAM28 over-expression, the protein expression of vimentin in the A-498 cells of M145 group was significantly increased (P<0.05), and the protein expression of E-cadherin was significantly decreased (P<0.05). The results of dual-lucife-irasei reporter assay showed that ADAM28 was a downstream target gene of miR-145. CONCLUSION: miR-145 may inhibit the expression of EMT-related proteins through the downstream target gene ADAM28 and inhibit the EMT process of renal cancer A-498 cells.     

淮安市食品产业转型升级与高职教育发展关联研究

高职教育发展影响着着区域产业的发展,区域产业的发展又决定着对高职人才的需求.文章运用教育学和产业经济学有关理论,探讨淮安食品产业发展存在的问题和淮安市食品大类高职教育现状,基于两者紧密关联的角度,从政府、学校、企业三个维度,共提出了八条针对性措施.