Identification of Differentially Expressed Genes and Lipid Metabolism Signaling Pathways between Muscle and Fat Tissues in Broiler Chickens

In this study, signaling pathways and key differentially expressed genes (DEGs) involved in lipid metabolism in muscle and fat tissues were investigated. Muscle and abdominal fat tissues were obtained from 35-day-old female broilers for RNA sequencing. DEGs between muscle and fat tissues were identified. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of DEGs were performed. A total of 6130 DEGs were identified to be significantly enriched in 365 GO terms, most of which were involved in biological processes, cellular components, and molecular functions in muscle and fat tissues. Three important lipid signaling pathways (pyruvate metabolism, the insulin signaling pathway, and the adipocytokine signaling pathway) were identified among the fat and muscle tissues of broilers. The key common DEGs in these pathways included phosphoenolpyruvate carboxykinase 2 (PCK2), acetyl-CoA carboxylase 1 alpha and beta (ACACA and ACACB), and the mitogen-activated protein kinase (AMPK) gene family. Hence, our findings revealed the pathways and key genes and gene families involved in the regulation of fat deposition in the muscle and fat tissues of broilers.     

Effect of L-lysine Added in Diluent on the Fresh Semen Quality of Dorper Sheep

In order to explore the effect of different L-lysine levels added in semen dilution on the fresh Dorper sheep quality stored at liquid state,3 health Dorper ram were used to collect semen which were equal-packaged and added to solution containing different levels (0,0.1,0.2,0.3,0.4 and 0.5 g) L-lysine in 120 mL diluent and at 0,6,24,30,48,54,72 and 78 h,the sperm motility,membrane integrity and acrosome integrity were detected.The results showed that the sperm motility in groups added 0,0.1 and 0.2 g L-lysine were higher than other groups,and that in 0.4 and 0.5 g L-lysine groups decreased faster than other groups;The sperm motility in 0.5 g L-lysine group reduced to 0 at 30 h,and it reduced to 0 at 48 h in the group added 0.4 g L-lysine;The effective survival time and survival index in the group added 0.1 g L-lysine was higher than other groups (P< 0.05);The membrane integrity of 0.1 g group was the highest,that had no significant difference with control group from 0 to 54 h (P >0.05),while after 72 h the two groups had significant differences (P< 0.05).The membrane integrity in 0.4 and 0.5 g groups were the worst,and that was significant different with other groups (P< 0.05);The acrosome integrity of 0.1 g group was the highest which had no significant difference with control group (P >0.05),0.4 and 0.5 g groups were the worst,the difference between them was not significant (P >0.05),while was extremely significant difference with other groups except for 0 and 6 h (P< 0.01).The results suggested that dilution added a high concentration of L-lysine could inhibit sperm quality,when the count of L-lysine was more than 0.2 g,sperm quality was significantly decreased,adding 0.1 g L-lysine could improve Dorper sheep fresh sperm quality stored at liquid state.     

聚脲喷枪混合室的设计和FLUENT仿真分析

为提高聚脲喷涂喷枪的混合效果，基于撞击流理论设计了一种新型聚脲喷涂喷枪的混合室。混合室采用两级撞击混合，分别采用两组T型和两组Y型对撞混合形式。利用SolidWorks设计混合室的结构模型后，利用FLUENT软件，采用冷、热水在混合室内撞击混合、对流传热的方法进行仿真模拟，并且计算了流体在混合室内的微观混合时间。结果表明:合理增加撞击流混合室的混合级数和采用多组T型、Y型对撞混合形式可增益混合效果；撞击流混合室的主要混合区是在撞击区；流体在喷枪内停留的总时间约为5.71 ms，满足具有反应迅速这一特性的材料的混合要求；微观混合时间小于1 ms，混合效果优异。      

蝴蝶兰切花花被不同开放阶段酶活性的变化

以蝴蝶兰鲜切花为试材,对切花花被不同生理阶段3种酶活性的变化进行测量分析,以期为蝴蝶兰切花寿命的延长提供参考依据。结果表明:萼片和花瓣中的过氧化物酶(POD)从花苞期到半开期酶活性变化不明显,从半开期到衰老期酶活性显著升高。花瓣中的超氧化物歧化酶(SOD)从花苞期到半开期酶活性升高,从半开期到衰老期酶活性显著降低;萼片中的超氧化物歧化酶活性变化不显著但也呈先上升后下降趋势。花瓣中的中性蛋白酶(NP)从花苞期到半开期酶活性呈上升趋势,半开期到盛开期呈下降趋势,盛开期到衰老期呈上升趋势;花萼中的中性蛋白酶从花苞期到盛开期酶活性呈下降趋势,从盛开期到衰老期呈上升趋势;总体来看中性蛋白酶的活性呈先下降后上升趋势。     

GIS技术在农业信息化中的应用

气候、资源和水土等地理信息是农业发展的重要影响因素,通过GIS技术分析复杂多变的地理信息,可以进行有效的信息管理、分辨和测试。阐述了GIS技术的概念及其在农业信息化中的应用,并对GIS技术发展前景进行了展望。     

TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE2分泌的影响

[目的] 进一步探讨牛磺鹅去氧胆酸（taurochenodeoxycholic acid,TCDCA）在抗炎免疫方面的潜在调节作用。[方法] 以AA大鼠成纤维样滑膜细胞作为研究对象,采用ELISA方法检测TCDCA和IL-1β作用下AA大鼠成纤维样滑膜细胞上清液中PGE₂的含量,分析TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE₂分泌情况的影响。[结果] TCDCA能够对IL-1β刺激下AA大鼠纤维样滑膜细胞PGE₂的分泌产生下调作用（P<0.05）。[结论] TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE₂的分泌具有抑制作用,为TCDCA在兽医临床应用提供依据。     

基于LabVIEW分布式农业大棚设计

介绍一种基于LabVIEW的分布式农业大棚，在大棚不同区域设置终端节点，负责光照度、空气温湿度、CO2浓度和土壤湿度数据的采集和上传，同时接收集中器转发的指令，根据指令控制不同区域喷淋头、加热器、排风扇和遮阳板的动作，实现对各区域不同作物生长环境的分布精准调节。通过多点分布式监控，为大棚分区种植的每种作物提供最适合的生长环境，以满足职业院校农业类专业学生的实践需求。      

猪用加味玉屏风缓释微丸水提醇沉工艺研究

目的:考察猪用加味玉屏风缓释微丸的水提醇沉工艺。方法:以黄芪甲苷的含量及干膏得率作为评价指标,选用L9(34)进行正交试验。结果:猪用加味玉屏风缓释微丸最佳水提取工艺为药材分别加12倍量水,提取2次,提取时间分别为0.5 h;最佳醇沉工艺为浓缩药液相对密度1.15,加醇浓度70%,静置时间为12 h。结论:优选出的水提醇沉工艺科学、合理。     

试验统计方法课程教学改革探索与实践

介绍了试验统计方法课程的基本理论体系和教学目标,指出了试验统计方法课程教学中存在的主要问题,并提出了课程教学改革理念及思路,以为试验统计方法课程教学改革提供参考。