全文获取类型
收费全文 | 132241篇 |
免费 | 7635篇 |
国内免费 | 80篇 |
专业分类
林业 | 6203篇 |
农学 | 4871篇 |
基础科学 | 873篇 |
16828篇 | |
综合类 | 18601篇 |
农作物 | 4849篇 |
水产渔业 | 7176篇 |
畜牧兽医 | 70915篇 |
园艺 | 1717篇 |
植物保护 | 7923篇 |
出版年
2019年 | 1289篇 |
2018年 | 2186篇 |
2017年 | 2471篇 |
2016年 | 2310篇 |
2015年 | 1957篇 |
2014年 | 2377篇 |
2013年 | 5374篇 |
2012年 | 4122篇 |
2011年 | 5006篇 |
2010年 | 3288篇 |
2009年 | 3240篇 |
2008年 | 4777篇 |
2007年 | 4550篇 |
2006年 | 4436篇 |
2005年 | 3904篇 |
2004年 | 3726篇 |
2003年 | 3765篇 |
2002年 | 3456篇 |
2001年 | 4899篇 |
2000年 | 4683篇 |
1999年 | 3715篇 |
1998年 | 1470篇 |
1997年 | 1499篇 |
1996年 | 1321篇 |
1995年 | 1512篇 |
1994年 | 1336篇 |
1993年 | 1315篇 |
1992年 | 2773篇 |
1991年 | 2932篇 |
1990年 | 2922篇 |
1989年 | 2796篇 |
1988年 | 2548篇 |
1987年 | 2563篇 |
1986年 | 2518篇 |
1985年 | 2372篇 |
1984年 | 1946篇 |
1983年 | 1609篇 |
1982年 | 1132篇 |
1979年 | 1703篇 |
1978年 | 1316篇 |
1976年 | 1119篇 |
1975年 | 1206篇 |
1974年 | 1408篇 |
1973年 | 1376篇 |
1972年 | 1345篇 |
1971年 | 1288篇 |
1970年 | 1279篇 |
1969年 | 1214篇 |
1968年 | 1090篇 |
1967年 | 1146篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
981.
Flanagan ML Parrish CR Cobey S Glass GE Bush RM Leighton TJ 《Zoonoses and public health》2012,59(3):155-163
Zoonotic disease surveillance is typically triggered after animal pathogens have already infected humans. Are there ways to identify high‐risk viruses before they emerge in humans? If so, then how and where can identifications be made and by what methods? These were the fundamental questions driving a workshop to examine the future of predictive surveillance for viruses that might jump from animals to infect humans. Virologists, ecologists and computational biologists from academia, federal government and non‐governmental organizations discussed opportunities as well as obstacles to the prediction of species jumps using genetic and ecological data from viruses and their hosts, vectors and reservoirs. This workshop marked an important first step towards envisioning both scientific and organizational frameworks for this future capability. Canine parvoviruses as well as seasonal H3N2 and pandemic H1N1 influenza viruses are discussed as exemplars that suggest what to look for in anticipating species jumps. To answer the question of where to look, prospects for discovering emerging viruses among wildlife, bats, rodents, arthropod vectors and occupationally exposed humans are discussed. Finally, opportunities and obstacles are identified and accompanied by suggestions for how to look for species jumps. Taken together, these findings constitute the beginnings of a conceptual framework for achieving a virus surveillance capability that could predict future species jumps. 相似文献
982.
983.
984.
985.
Resistance to benzimidazole and macrocyclic lactone anthelmintics in cattle nematodes in Argentina 总被引:3,自引:0,他引:3
Anziani OS Suarez V Guglielmone AA Warnke O Grande H Coles GC 《Veterinary parasitology》2004,122(4):303-306
In April 2003, persistent scouring and ill-thrift that was reported in calves form an intensive beef rearing operation in central Argentina despite treatments with benzimidazole and ivermectin. In order to conduct a controlled faecal egg count reduction test on this herd, 40 calves 5-8-months-old were selected on the basis that they had a nematode eggs per gram (epg) of faeces count greater than 150. Animals were divided into four groups (1-4) of 10 calves. Calves of groups 1-3 were treated, respectively, with subcutaneous injection of ivermectin (200 mcg/kg), ricobendazole (4 mg/kg) and levamisole (7.5 mg/kg), while calves of group 4 remained as untreated controls. The egg count reductions carried out 10 days later were lower than 15% in calves treated with ivermectin and ricobendazole, but 100% in animals receiving levamisole. Pooled post-treatment faecal cultures showed larval percentages of 92 and 95 for Haemonchus and 8 and 5 for Cooperia in the faeces of calves treated with ivermectin and ricobendazole, respectively. This is the first reported case of Haemonchus parasiting cattle showing simultaneous resistance to avermectins and benzimidazole type anthelmintics. 相似文献
986.
Nagamine MK Sanches DS Pinello KC Torres LN Mennecier G Latorre AO Fukumasu H Dagli ML 《Veterinary research communications》2011,35(6):391-399
Mast cell tumor (MCT) is one of the most prevalent neoplasms that affect skin and soft tissue in dogs. Because mast cell tumors
present a great variety of clinical appearance and behavior, their treatment becomes a challenge. Trichostatin A (TSA), an
antifungal antibiotic, has shown inhibitory effects on the proliferation and induction of apoptosis in various types of cancer
cells. In order to evaluate the potential of trichostatin A as a therapeutic drug, cells of grade 3 MCT were cultured and
treated with concentrations of 1 nM to 400 nM of TSA. MTT assay and trypan blue exclusion assays were performed to estimate
cell growth and cell viability, and cell cycle analysis was evaluated. TSA treatment showed a reduction in numbers of viable
cells and an increase of cell death by apoptosis. The cell cycle analysis showed an increase of hypodiploid cells and a reduction
of G0/G1 and G2/M –phases. According to these results, trichostatin A may be an interesting potential chemotherapeutic agent
for the treatment of canine MCT. 相似文献
987.
Effect of dietary Schizochytrium microalga oil and fish oil on plasma cholesterol level in rats 下载免费PDF全文
T. Komprda O. Škultéty S. Křížková G. Zorníková V. Rozíková R. Krobot 《Journal of animal physiology and animal nutrition》2015,99(2):308-316
The purpose of the study was to test the hypothesis that the dietary oils with different content of n‐3 polyunsaturated fatty acids (PUFA) eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA) affect plasma lipid level in rats in a different degree. The diets with 6% of fish oil (FO) and Schizochytrium microalga oil (SchO; EPA+DHA content in the diets 9.5 + 12.3 and 2.6 + 29.5% of the sum of total fatty acids, respectively) were used; the diet with 6% of safflower oil (high content of n‐6 PUFA linoleic acid, 65.5%; EPA+DHA content 0.7 + 0.9%) was used as a control. The difference between FO and SchO was established only in the case of plasma triacylglycerol (TAG) level: plasma TAG of the FO‐fed rats did not differ from the control rats (p > 0.05), while SchO decreased (p < 0.05) plasma TAG to 46% of the control. On the other hand, FO and SchO decreased (p < 0.05) total plasma cholesterol (TC) in rats in the same extent, to 73% of the control. Regarding the underlying mechanisms for the TC decrease, both SchO and FO up‐regulated hepatic Insig‐1 gene (181 and 133% of the control; p < 0.05), which tended (p = 0.15 and p = 0.19 respectively) to decrease the amount of hepatic nSREBP‐2 protein (61 and 66% of the control). However, neither SchO nor FO influenced hepatic 3‐hydroxy‐3‐methyl‐glutaryl‐CoA reductase gene expression (p > 0.05); SchO (but not FO) increased (p < 0.05) low‐density lipoprotein receptor mRNA in the liver. It was concluded that the decrease of total plasma cholesterol might be caused by an increased cholesterol uptake from plasma into the cells (in the case of SchO), but also by other (in the present study not tested) mechanisms. 相似文献
988.
989.
990.
C P Moore J B Shaner R M Halenda C S Rosenfeld W K Suedmeyer 《Journal of zoo and wildlife medicine》1999,30(3):423-430
A 5-wk-old female dromedary camel (Camelus dromedarius) was clinically diagnosed with bilateral corneal dermoids, incomplete congenital cataracts, a left persistent hyaloid artery (PHA), and a ventricular septal defect (VSD). The corneal dermoids were removed by lamellar keratectomy, and vision improved in the left eye. Thirteen months after dermoid surgery, the calf was presented for enlargement of the right eye. Glaucoma was confirmed in the right eye, and corneal fibrosis and cataract were noted in the left eye. Persistence of the VSD was confirmed by cardiac ultrasonography. The calf was euthanized, and necropsy findings confirmed VSD. Histopathologic examination revealed bilateral corneal thinning and fibrosis, cataracts with retrolental fibroplasia, and retinal dysplasia. Additional changes in the right globe were anterior segment dysgenesis, ruptured lens capsule, chronic phacoclastic uveitis, and retinal separation. The PHA was confirmed in the left eye. 相似文献