Root produced DHZR-, ZR- and IPA-like cytokinins in xylem sap in relation to coppice shoot initiation and growth in cloned trees of Betula pubescens

Six-year-old cloned Betula pubescens Ehrh. trees, grown outdoors at 65 degrees 01' N, were cut on six dates during the growing season to study coppice shoot development in relation to root-produced cytokinin-like compounds. Bleeding sap was collected over timed intervals for two days after cutting, and endogenous cytokinin-like compounds were measured by ELISA assay in HPLC-purified fractions of xylem sap. Initiation and development of coppice shoots on the clonally propagated plants were comparable to those in seedlings. Coppice shoot initiation was affected by the time of cutting, diminishing significantly after June. Of the cytokinin-like compounds detected in the xylem sap, zeatin riboside-like (ZR) compounds were present in the highest concentrations, and the concentrations of dihydrozeatin riboside-like (DHZR) and isopentenyladenoside-like (IPA) compounds were approximately one third and one eighth of the ZR concentrations, respectively. The concentration of cytokinin-like compounds was positively correlated with xylem sap flow rate. The export of cytokinin-like compounds, especially DHZR- and ZR-types, was positively correlated with the initiation and elongation rate of coppice shoots, the number of lateral branches, and the radial growth of the more slowly growing coppice shoots. The export of cytokinin-like compounds collected immediately after cutting may represent the basal value for each tree. This value is probably affected by the size and activity of the root system and may be a relevant estimate for predicting the success of coppicing.     

Seasonal changes in bud dormancy in relation to bud morphology, water and starch content, and abscisic acid concentration in adult trees of Betula pubescens

Annual cycles of change in bud morphology, bud burst ability, abscisic acid (ABA) concentration, and starch and water content were studied in mid-crown terminal buds of short shoots and underground basal buds of Betula pubescens Ehrh. In particular, we investigated the roles of ABA and bud water content in the regulation of bud growth. Basal buds differed morphologically from terminal buds of short shoots in that their leaf initials did not develop into embryonic foliage leaves and their total size did not increase significantly during summer. Bud burst ability, measured by forcing detached short shoots and stumps under controlled conditions, was maintained in the basal buds throughout the year, whereas the terminal buds of short shoots remained dormant until October, thereafter their bud burst ability increased gradually and reached a maximum in March-April. The ABA concentration of the basal buds was relatively constant throughout the sampling period (1-3 micro g g(DW) (-1)), whereas that of the terminal buds of short shoots, which was much higher (5-10 micro g g(DW) (-1)), showed a distinct seasonal cycle with a maximum from August to November. Bud ABA concentration decreased during the first 10 days of forcing, especially in basal buds. In both bud types, the amount of starch increased toward the autumn, declined in November, and was negligible in the terminal buds of short shoots between January and March, but in April, the amount was high again in both bud types. Water content varied characteristically in both bud types, although more distinctly in the terminal buds of short shoots, with an increase in spring before bud burst and a decrease during the summer until September. The significant morphological and physiological differences between the mid-crown terminal buds of short shoots and the underground basal buds may partly explain the characteristic growth habit of the basal buds and their development into coppice shoots after cutting the tree. The results also indicate a role for ABA in maintaining dormancy of the terminal buds of short shoots and emphasize the relationship between tissue water status and ABA concentration.     

稀土对亚麻产质量效果的研究

对黑龙江省亚麻主要栽培品种──黑亚三号,采用稀土硝酸盐—“农乐一号”,有效成分为３８．７％,对每公顷种予用６００ｇ稀土拌种,能提高亚麻生理功能,并对产质量构成因素有重要促进作用。说明用适量的稀土拌种对亚麻有增加产量,提高纤维品质的效果。