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排序方式: 共有1166条查询结果,搜索用时 15 毫秒
91.
92.
Chen J de Felipe KS Clarke M Lu H Anderson OR Segal G Shuman HA 《Science (New York, N.Y.)》2004,303(5662):1358-1361
Legionella pneumophila, the bacterial agent of legionnaires' disease, replicates intracellularly within a specialized vacuole of mammalian and protozoan host cells. Little is known about the specialized vacuole except that the Icm/Dot type IV secretion system is essential for its formation and maintenance. The Legionella genome database contains two open reading frames encoding polypeptides (LepA and LepB) with predicted coiled-coil regions and weak homology to SNAREs; these are delivered to host cells by an Icm/Dot-dependent mechanism. Analysis of mutant strains suggests that the Lep proteins may enable the Legionella to commandeer a protozoan exocytic pathway for dissemination of the pathogen. 相似文献
93.
The brain keenly depends on glucose for energy, and mammalians have redundant systems to control glucose production. An increase in circulating glucose inhibits glucose production in the liver, but this negative feedback is impaired in type 2 diabetes. Here we report that a primary increase in hypothalamic glucose levels lowers blood glucose through inhibition of glucose production in rats. The effect of glucose requires its conversion to lactate followed by stimulation of pyruvate metabolism, which leads to activation of adenosine triphosphate (ATP)-sensitive potassium channels. Thus, interventions designed to enhance the hypothalamic sensing of glucose may improve glucose homeostasis in diabetes. 相似文献
94.
Jadzinsky PD Calero G Ackerson CJ Bushnell DA Kornberg RD 《Science (New York, N.Y.)》2007,318(5849):430-433
Structural information on nanometer-sized gold particles has been limited, due in part to the problem of preparing homogeneous material. Here we report the crystallization and x-ray structure determination of a p-mercaptobenzoic acid (p-MBA)-protected gold nanoparticle, which comprises 102 gold atoms and 44 p-MBAs. The central gold atoms are packed in a Marks decahedron, surrounded by additional layers of gold atoms in unanticipated geometries. The p-MBAs interact not only with the gold but also with one another, forming a rigid surface layer. The particles are chiral, with the two enantiomers alternating in the crystal lattice. The discrete nature of the particle may be explained by the closing of a 58-electron shell. 相似文献
95.
Meshik A Mabry J Hohenberg C Marrocchi Y Pravdivtseva O Burnett D Olinger C Wiens R Reisenfeld D Allton J McNamara K Stansbery E Jurewicz AJ 《Science (New York, N.Y.)》2007,318(5849):433-435
To evaluate the isotopic composition of the solar nebula from which the planets formed, the relation between isotopes measured in the solar wind and on the Sun's surface needs to be known. The Genesis Discovery mission returned independent samples of three types of solar wind produced by different solar processes that provide a check on possible isotopic variations, or fractionation, between the solar-wind and solar-surface material. At a high level of precision, we observed no significant inter-regime differences in 20Ne/22Ne or 36Ar/38Ar values. For 20Ne/22Ne, the difference between low- and high-speed wind components is 0.24 +/- 0.37%; for 36Ar/38Ar, it is 0.11 +/- 0.26%. Our measured 36Ar/38Ar ratio in the solar wind of 5.501 +/- 0.005 is 3.42 +/- 0.09% higher than that of the terrestrial atmosphere, which may reflect atmospheric losses early in Earth's history. 相似文献
96.
Ke Jin Wim M. Cornelis Wouter Schiettecatte Junjie Lu Yuqing Yao Huijun Wu Donald Gabriels Stefaan De Neve Dianxiong Cai Jiyun Jin Roger Hartmann 《Soil & Tillage Research》2007,96(1-2):131-144
Field experiments were carried out to study the effects of different soil management practices on the water balance, precipitation use efficiency (PUE), and crop yield (i.e. winter wheat and peanut) on a loess soil near Luoyang (east edge of the Chinese Loess Plateau, Henan Province, China). Field plots were set up in 1999 including following soil management practices: subsoiling with mulch (SS), no-till with mulch (NT), reduced tillage (RT), two crops per year (i.e. winter wheat and peanut, TC), and a conventional tillage control (CT). The field plots were equipped to monitor all components of the soil–water balance except evapotranspiration, which was computed by solving the water balance equation. The results showed that although soil management had smaller influence on the magnitude of the water balance components than did precipitation variations, small influences of the applied soil management practices on water conservation during the fallow period can greatly affect winter wheat yield. SS increased consistently precipitation storage efficiency (PSE) and PUE over the 5 years compared to CT except during the wettest year. NT also had a noticeable effect on postharvest water storage during the fallow period; however, the influence on yield of NT depended on the amount of precipitation. TC lowered the winter wheat yield mainly due to the unfavorable soil moisture conditions after growing peanut in summer; however, the harvested peanut gained an extra profit for the local farmer. No matter which kind of soil management practices was adapted, PSE never exceeded 41.6%, which was primarily attributed to high evapotranspiration. From data of five consecutive agricultural years between 2000 and 2005, it could be concluded that SS resulted in the highest PSE, PUE and crop yield. TC also showed promising results considering the economic value of the second crop. NT performed slightly less as SS. CT gave intermediate results, whereas RT was the worst alternative. 相似文献
97.
A nitrogen dynamic hydroponic culture on performance and quality of water spinach (Ipomoea aquatica)
AbstractIn view of the unreasonable application of chemical fertilizers in agriculture and the groundwater pollution caused by nitrogen (N) leaching, a nitrogen dynamic hydroponic culture was used to simulate the dynamics variation of ammonium nitrogen (NH4-N) and nitrate nitrogen (NO3-N) in the leaching loss soil. Solutions with different ratio of NO3-N and NH4-N (100:0, 70:30, 50:50, 30:70 and 0:100) were prepared as well as the same solutions that not containing NO3-N .Water spinach was chosen to culture in the two solutions that differ from NO3-N every two (E2) or five days (E5) to observe the growth, quality and nutrient solution uptake. In terms of the growth, uptake of N source, N use efficiency and the chemical indicators, plants grown in the balanced solution all showed the best results. The nitrogen dynamic hydroponic culture showed some differences between E2 and E5 in some aspects. Under the same N source level, plants in E2 showed a better growth and higher NO3-N uptake than E5. On the other hand, the uptake of NH4-N seemed to be affected significantly by the interchanged frequency, which showed the same variations of glutamine synthetase activity. The activity of nitrate reductase and glutamine synthetase showed the coexist of NO3-N and NH4-N could play synergistic effect. It is not recommended to supplement NO3-N frequently in the case of N leaching loss, which has little impact on the growth and may lead to the hard taste and groundwater pollution. 相似文献
98.
99.
Amy M. Treonis Roger Cook Lorna Dawson Susan J. Grayston Tony Mizen 《Biology and Fertility of Soils》2007,43(5):541-548
We studied the effects of the root endoparasitic nematode Heterodera trifolii on rhizodeposition and the root architecture of white clover (Trifolium repens). Rhizosphere solutions were collected from the root systems of plants growing with and without H. trifolii (200 juveniles per inoculated plant) in sand-based microlysimeters. The organic carbon (C) content of these solutions was
analyzed, and they were applied to plant-free soils to investigate microbial responses. Although plant biomass was unaffected
by nematodes, the architecture of the root systems was significantly altered, with a decrease in overall root length and an
increase in the density of lateral branches from the primary root. The presence of nematodes reduced the concentration of
organic compounds in the rhizosphere solutions but only on the final sampling date (75 days). Analysis of microbial signature
phospholipid fatty acids revealed no change in the structure of the microbial communities in soils to which rhizosphere solutions
were applied. However, these microorganisms did respond with changes in substrate utilization patterns (community-level physiological
profiles). Microbes in soils that received rhizosphere solutions from the nematode-infected clover showed lower utilization
of most substrates but higher utilization of oligosugars. These responses appear to be related to changes in roots and rhizodeposition
associated with nematode infection of clover roots. The results of this study suggest that root herbivory can negatively impact
carbon-limited soil microbial communities via changes in root architecture that moderate rhizodeposition. 相似文献
100.