Insecticide resistance compromises the control of Aedes aegypti in Bangladesh

BACKGROUND

With no effective drugs or widely available vaccines, dengue control in Bangladesh is dependent on targeting the primary vector Aedes aegypti with insecticides and larval source management. Despite these interventions, the dengue burden is increasing in Bangladesh, and the country experienced its worst outbreak in 2019 with 101 354 hospitalized cases. This may be partially facilitated by the presence of intense insecticide resistance in vector populations. Here, we describe the intensity and mechanisms of resistance to insecticides commonly deployed against Ae. aegypti in Dhaka, Bangladesh.

RESULTS

Dhaka Ae. aegypti colonies exhibited high-intensity resistance to pyrethroids. Using CDC bottle assays, we recorded 2–24% mortality (recorded at 24 h) to permethrin and 48–94% mortality to deltamethrin, at 10× the diagnostic dose. Bioassays conducted using insecticide–synergist combinations suggested that metabolic mechanisms were contributing to pyrethroid resistance, specifically multi-function oxidases, esterases, and glutathione S-transferases. In addition, kdr alleles were detected, with a high frequency (78–98%) of homozygotes for the V1016G mutation. A large proportion (≤ 74%) of free-flying and resting mosquitoes from Dhaka colonies survived exposure to standard applications of pyrethroid aerosols in an experimental free-flight room. Although that exposure affected the immediate host-seeking behavior of Ae. aegypti, the effect was transient in surviving mosquitoes.

CONCLUSION

The intense resistance characterized in this study is likely compromising the operational effectiveness of pyrethroids against Ae. aegypti in Dhaka. Switching to alternative chemical classes may offer a medium-term solution, but ultimately a more sustainable and effective approach to controlling dengue vectors is required. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.     

Acute and Subacute Metabolic and Endocrine Effects of Clenbuterol in Female Pigs

The aim of the study was to assess the relationship between acute and subacute metabolic and endocrine effects after intravenous administration of the ₂-adrenergic agonist clenbuterol in a growth-promoting dose to female pigs. Acute metabolic and endocrine effects were assessed by measuring the blood glucose, serum insulin and nonesterified fatty acid (NEFA) concentrations during 300 min after a single administration of clenbuterol. Significantly higher serum insulin and NEFA concentrations (19.90±2.50 U/ml, p<0.01, and 0.69±0.04 mmol/L, p<0.001, respectively) were measured 30 min after the preprandial administration of clenbuterol in female pigs. Over the same period, the levels of blood glucose (4.42±0.30 mmol/L) showed no difference from those of control pigs. The postprandial serum NEFA concentration decreased moderately during 210 min after feeding. Postprandial blood glucose and insulin concentrations increased and reached maximal levels 120 min after clenbuterol administration (10.91±0.60 mmol/L and 85.22±7.24 U/ml, respectively), and returned to basal levels at 300 min (4.20±0.21 mmol/L and 7.75±1.60 U/ml, respectively) after the administration of clenbuterol. Subacute metabolic and endocrine effects were assessed by measuring the blood glucose, serum insulin and NEFA concentrations for 21 days after the repeated doses of clenbuterol. In addition, the influence of clenbuterol administration on the endocrine regulation of the onset of the next expected oestrus in female pigs was assessed by measuring their serum 17-oestradiol and progesterone concentrations. Blood glucose, serum insulin and NEFA concentrations after the last administration of clenbuterol did not differ significantly from those in control animals. The onset of the next expected oestrus occurred regularly without any significant difference in serum 17-oestradiol or progesterone concentrations between the treated (9.83±2.60 pg/ml and 0.15±0.03 ng/ml) and control pigs (8.52±2.70 pg/ml and 0.25±0.06 ng/ml). The study results suggest the duration of intravenous administration of clenbuterol in a growth-promoting dose necessary to influence the metabolic and endocrine activities in female pigs.     

Properties of anaerobically digested and composted municipal solid waste assessed by linking soil mesofauna dynamics and nitrogen modelling

We have studied the effect of anaerobically digested (ADMSW) and composted municipal solid waste (CMSW) on mineralization and foodweb dynamics to verify the hypothesis that ADMSW would immobilize N right after addition to soil in contrast to addition with CMSW. Another hypothesis was that the mesofauna (enchytraeids and microarthropods) would stimulate N release from the decomposer community. We measured excretion of -N and urea-N from the mesofauna and hypothesized that enchytraeids would release urea. ADMSW and CMSW were amended to pots with sandy loam and barley. The pots were divided into treatments with or without mesofauna. Mesofauna, plant N and biomass, soil N and ergosterol (fungal biomass) were measured over a 113-day period of four equidistant samplings. Soil respiration, N mineralization and N release by the mesofauna were modelled from concurrent studies. ADMSW- and CMSW-treated soils initially (<20 days) immobilized N. The amendments did not increase plant growth substantially, and this was probably due to N-limitation in the early stages of plant growth. Enchytraeid abundance was about three times higher in ADMSW- than CMSW-treated soils, indicating that ADMSW contained more labile compounds, bacteria, and microfauna. The mesofauna did not affect N-content, but the cumulated -N excreted by the mesofauna was estimated to be substantial and about one-fifth of total plant N in ADMSW. An explanation for this discrepancy might be that in the absence of the mesofauna, other members of the detrivore and microbivore community performed the mesofauna’s function. Neither enchytraeids nor microarthropods excreted urea.     

Performance assessment of irrigation water management of heterogeneous irrigation schemes: 1. A framework for evaluation

Studies of the performance assessment of irrigation schemes have gained momentum since the late 1980s due to the common perspective that the resources (land and water) in irrigation schemes are not being managed appropriately. In this paper irrigation water management is considered as one of the activities of the irrigation scheme. Three phases of irrigation water management namely planning, operation and evaluation are identified. A framework for the performance assessment of irrigation water management in heterogeneous irrigation schemes is proposed in this paper, based on earlier studies made in this direction. The paper presents two types of allocative measures (productivity and equity) and five types of scheduling measures (adequacy, reliability, flexibility, sustainability and efficiency), together with the methodologies for estimating these for the scheme as a whole during different phases of irrigation water management.     

Sugar beet and Barley Yields in Relation to Bacillus polymyxa and Bacillus megaterium var. phosphaticum Inoculation

Microbiological fertilizers are an important part of environment friendly sustainable agricultural practices. A study was conducted in order to investigate seed inoculation of sugar beet (cv. Sonja) and barley (cv. Tokak), with nitrogen fixing (Bacillus polymyxa) and phosphate dissolving (Bacillus megaterium var. phosphaticum) bacteria in comparison to control and mineral fertilizer application in the green house and in the field at two sites in 1996 and 1997 in Erzurum (29^o 55' N and 41^o 16' E with an altitude of 1950 m), Turkey. In the green house trial bacterial inoculation of seeds increased sugar beet root and barley seed yields compared with control. B. polymyxa was more effective, but dual inoculations gave increases of 19.0 and 25.9% respectively. In the field as an average of sites and years sugar beet root yields were increased by 12.0%, 7.5% and 16.5% while barley seed yields were increased by 15.0%, 8.4% and 18.2% respectively with B. polymyxa, B. megaterium var. phosphaticum and dual applications over control treatment. Other yield and yield components were also enhanced especially by dual applications. However, increases in yield and yield components were lower than or comparable to mineral fertilizer application. The beneficial effect of bacterial inoculation was higher in well irrigated pot trials and at the site of rich clayey soils. In conclusion, microbial inoculation of seeds with Bacillus polymyxa and B. megaterium var. phosphaticum, alone or in dual combinations, may substitute costly NP fertilizers in sugar beet and barley production even in cold highland areas such as in Erzurum.     

The almond Sf haplotype shows a double expression despite its comprehensive genetic identity

Since self-compatibility has become the primary objective of most almond breeding programmes, search for new self-compatibility sources has acquired a great importance in almond research. The local Spanish cultivar ‘Vivot’, identified as showing the genotype S₂₃S_f, thus presumably self-compatible, was found to be unexpectedly self-incompatible in spite of the presence of the S_f allele, as also observed in other almond cultivars. However, not only the coding sequences of both the S_f-RNase and the SFB_f of ‘Vivot’ and ‘Blanquerna’, a confirmed self-compatible cultivar, were identical, but also the 5′ regulatory sequence of the S_f-RNase of both. Thus, the reason for the different expression of the S_f is independent of the complete genetic identity found in the whole chromosome region bordering the S-locus in the almond cultivars sharing the S_f allele.     

The effect of N and NaCl on growth,yield, and nitrate content of salad rocket (Eruca sativa Mill.)

Hydroponic production of rocket as a salad vegetable has become increasingly important in recent years. Rocket is known to be a high nitrate (NO₃^–)-accumulating vegetable, which can be grown throughout the year. In the present study, rocket was grown in a floating hydroponic system at three levels of nitrogen (N) and sodium chloride (NaCl). The highest yield was obtained at 14 mM N, whereas the yield was lower at 20 mM and 40 Mm NaCl. Leaf elongation was more sensitive to salinity than leaf differentiation. Adding NaCl to the nutrient solution increased the relative chlorophyll content. Na⁺ and Cl^– concentrations increased as salinity increased. NO₃^? levels in fresh biomass increased with increased amounts of NO₃^? in the nutrient solution, and plants at 18 mM N were able to maintain a higher NO₃^? : Cl^? ratio than those at 10 mM N.     

The prevalence of <Emphasis Type="Italic">Cryptosporidium</Emphasis> species in diarrhoeic lambs in Kars province and potential risk factors

This study was carried out to determine the prevalence of Cryptosporidium species in diarrhoeic lambs and investigate some risk factors in Kars province (Northeastern region of Anatolia) in Turkey. Four hundred faecal samples were taken from the rectums of clinically diarrhoeic and aged to 1-month-old lambs from 34 sheep farms in 20 villages in March-April 2007 and examined by using the modified acid-fast staining technique. The prevalence of Cryptosporidium species was found as 38.8% (155/400). Cryptosporidium oocysts were detected in 90.0% (18/20) of villages and in 76.5% (26/34) of the sheep farms. Infection rates were detected as: 44.4% (67/151) in 1-week-old lambs, 37.5% (39/104) in 2-week-old lambs, 40.0% (38/95) in 3-week-old lambs, and 22.0% (11/50) in 4-week-old lambs. Farms classified according to their zoohygienic conditions and fine, average and bad conditioned farms were contaminated with Cryptosporidium with the percentages of 14.7%, 20.6% and 41.2%, respectively. Clinical cryptosporidiosis was determined in 35.0% of the villages (7/20) and in 29.4% of the sheep farms (10/34), Cryptosporidium oocysts were found in 81.3% of the lambs (91/112) in these farms. Cryptosporidiosis may be a major epidemiological significance in lambs in Kars province, and suggests that naturally infected lambs may be reservoirs of Cryptosporidiosis infections for calves even for humans too.     

Leaching of mineral nitrogen in the soil influenced by addition of compost and N-mineral fertilizer

This paper deals with the problem of mineral N leaching from arable lands due to the fertilization method. The influence of different doses of compost (50 and 100?Mg ha^?1) and N-mineral fertilizer (35-70-140 kg N ha^?1) on leaching of N_min in a lysimetric experiment with winter wheat. The area of our interest represents the main source of drinking water for the city of Brno and its neighborhoods. To demonstrate the effect of compost and mineral nitrogen addition, the lysimetric experiment was established there. Seven variants of the experiment with different fertilization schemes were studied during two vegetation seasons (2013 and 2014), each with three repetitions. The experiment was carried out in plastic experimental containers of 0.3 m diameter and 0.5 m height. Therefore, each lysimeter was the same size and was filled with 25 kg of subsoil and 25 kg of topsoil. The highest leaching of N_min was detected in the variant C2 where 140 kg N ha^?1 was applied, in both vegetation periods (5.97 kg N_min ha^?1 after the first vegetation period and 17.02 kg N_min ha^?1 after the second vegetation period). The positive effect of compost application (individually or in combination with the mineral N) on decrease in mineral N leaching was found during both vegetation period in comparison with variant C2. The highest doses of compost (100?Mg per ha) significantly decreased the concentration of mineral nitrogen in the soil eluate in both periods (3.03 kg N_min ha^?1 and 5.79 kg N_min ha^?1, respectively), by 197% and 293% in comparison with variant C2. There is evidence that the application of compost has a positive effect on the reduction of N_min leaching.     

阿苯达唑对自发性兔脑炎原虫病的治疗

为了有效地治疗兔脑炎原虫病,本研究根据脑炎原虫的生物学特点和对组织损伤的特征选用阿苯达唑进行了治疗试验。将28只3～4月龄隐性感染的獭兔,随机分为对照组和治疗组进行试验。治疗组按30mg／kg剂量（首次给药量为50mg／kg）,每隔12h给药1次,连续用药10d,停药1周为1个治疗期,共治疗3个疗程。对照组按正常饲养。每1疗程之后,均采血和尿液进行ELISA检测和尿沉渣虫体检查。结果,治疗组的病兔用阿苯达唑治疗3个疗程后,ELISA检测的D值均低于标准值（〈0．044）,即抗脑炎原虫血清抗体呈阴性反应,从尿沉渣中也不能检出虫体。而对照组病免的D值则大大高于正常值．从尿沉渣中均易检出虫,并有1只对照兔出现典型的神经症状,剖检后从脑组织中检出脑炎原虫性肉芽肿。经方差分析,2组间差异非常显著（P〈0．01）。试验证明,阿苯达唑对兔脑炎原虫有良好治疗作用,用药的时机以脑炎原虫还未进入脑组织时最合适,用药的剂量一定要保持有效的杀虫浓度。