Role of neutrophil elastase and myeloperoxidase in asthma of rats

AIM:To observe the changes of polymorphonuclear leukocytes (PMN), neutrophil elastase (NE) and myeloperoxidase (MPO) expression in rat asthma. METHODS:Eighteen rats were randomly divided into two groups on average, including asthma group and control group. The rat model of asthma was established by the ovalbumin (OVA) challenge methods. Blood PMN were isolated andpurified. The protein expression of MPO were detected by immunohistochemistry and chromatometry techniques. NE was detected by ELISA. RESULTS:(1) Immunohistochemistry showed that the levels of MPO expression around bronchus and in purified PMN in asthma group were significantly increased compared with those in control group (P<0.01). Activities of MPO were significantly increased in bronchoalveolar lavage fluid (BALF) and lung tissue in asthma group compared with those in control group (P<0.05, P<0.01). (2) Levels of NE were significantly increased in BALF and PMN in asthma group compared with control group (P<0.01). (3) Number of PMN were significantly higher in BALF, bronchus and lung tissue in asthma group than those in control group (P<0.01). CONCLUSION:Levels of PMN counting and expression of MPO and NEare significantly increased in experimental asthma rats. PMN may be involved in inflammation in asthma via increasing the levels of NE and MPO.     

不同果袋类型对紫花杧的套袋效果

以主栽品种紫花杧为试材,探讨不同果袋类型的套袋效果.结果表明:套袋可大幅度降低采收果的果面流胶和雨水污染,极显著地降低果面虫伤率.套袋可改变采收时的果面颜色,但不同果袋类型间存在差异.后熟完成时,果面均为品种固有颜色,不因果袋种类而改变.套袋能促进果实后熟转黄和果色均匀,能显著降低炭疽病的病果率,但不同果袋对蒂腐病的防效存在较大差异.套袋能提高可溶性固形物含量和减轻果实失重.供试品种用外黄内黑复合纸袋套袋效果最为理想.     

Etoposide induces apoptosis via mitochondrial signaling pathway with cytochrome c release in Jurkat leukemia cells

AIM:To investigate the molecular mechanisms of apoptosis and to elucidate the apoptosis signaling pathway triggered by etoposide in Jurkat human leukemia cells. METHODS:Apoptosis was detected using annexin V-FITC and propidium iodide (PI) staining, respectively, and annexin V-FITC positive cells and hypodiploid cells were analyzed by flow cytometry. Mitochondrial membrane potential (△Ψm) was detected using 3, 3-dihexyloxycarbocyanine iodide ［DiOC6(3)］ staining and △Ψm low cells were analyzed by flow cytometry. Preparation of cytosolic extracts and isolation of mitochondria were completed by centrifugation. Western blotting analysis was used to evaluate the level of cytochrome c, caspase-3, and poly (ADP-ribose) polymerase (PARP) expression. RESULTS:Etoposide induced apoptosis showing phosphatidylserine externalization and DNA fragmentation in a time-dependent manner and the apoptosis could be inhibited by a broad caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (zVAD.fmk). Collapse of △Ψm induced by etoposide preceded DNA fragmentation and phosphatidylserine externalization. In contrast, it was not blocked by zVAD.fmk. Etoposide caused cytochrome c release from mitochondria into cytosol, subsequent activation of caspase-3 (32 kD) presented with an intermediate (20 kD) and its active product (17 kD), and cleavage of full-length PARP (116 kD) into the so-called apoptotic 85 kD fragment. CONCLUSION:Etoposide-induced Jurkat cell apoptosis is initiated through mitochondria signaling pathway with cytochrome c release into cytoplasm and caspase is the ultimate executioner of cell apoptosis.     

抗香蕉枯萎病菌拮抗菌的鉴定及其定殖

通过逐步提高药物浓度方法,获得了抗利福平400μg/ml、对香蕉枯萎病菌拮抗活性稳定的T2WF和W10标记菌株。初步确定两菌株为芽孢杆菌属细菌。采用灌根接种法,在香蕉苗根际土和香蕉苗体内定殖结果表明,拮抗菌液接种浓度为5×104cfu/ml,两菌株均可从香蕉根际土和香蕉体内分离到,并在香蕉体内定殖和传导。在香蕉根际土、根部、球茎和假茎中,T2WF标记菌的菌量高峰分别出现在接种后5、5、11和3d,分别为707、437、273、117cfu/mg;而W10标记菌的菌量高峰分别出现在接种后5、3、5和5d,分别为784、260、253、110cfu/mg。两菌株在香蕉根际土和香蕉体内1~15d的消长动态均有一个共同的“由升到降”趋势。从数量和数量变动幅度上看,香蕉根部的菌量明显高于茎部的菌量。     

关于推进西双版纳天然橡胶初加工行业标准化管理的对策分析

分析了西双版纳天然橡胶制胶行业现状和行业标准化管理水平滞后的原因,提出了加快制胶行业标准化管理进程意见和措施。     

茶叶中茶多酚的特性及在食品中的应用

开发和利用天然产物中的抗氧化成分以取代合成抗氧化剂，已成为食品防腐保鲜的发展方向。本文介绍了茶叶中茶多酚的组成、理化特性以及生物活性，对茶多酚在食品中的应用，即肉制品抗氧化、保鲜，油脂抗氧化，果蔬保鲜，糕点防菌、抗氧化，以及在饮料中的应用进行了综述。     

英语学习中母语语音负迁移现象的分析

“母语语音负迁移”是中国学生在英语学习中普遍遇到的问题，其消极影响是显而易见的。本文从探讨汉语拼音的发音方法对英语语音的负迁移现象入手，揭示英汉语音的相似性与差异性。对英汉语音相似性与差异性的认知能促使中国学生在说英语时有意识地进行自我纠音，因此笔者认为在语音教学实践中引入英汉语音对比的相关知识及其研究成果将能有效地降低中国学生母语语音对目的语的干扰作用，从而提高语音教学质量。     

论坚持在和平共处五项原则的基础上同所有国家发展友好合作关系

在和平共处五项原则的指导下，中国在外交上取得了丰硕成果，为国内各项建设事业提供了良好的外部环境。和平共处五项原则仍然是当今处理国际关系必须遵循的基本准则。中国要按照大国是关键、周边是首要、发展中国家是基础、多边是重要舞台的外交总布局发展同所有国家的友好合作关系。     

新型自走式割灌机结构分析

介绍了一种新型自走式割灌机.该机由行走、割灌、驾驶台及排障四个核心部分组成,可以锯切直径为10～80 mm的灌木.本文对其行走部分和驾驶台进行了系统介绍,并对其主要部件进行了参数设计.     

论农民合作组织与市场农业发展

一家一户的家庭经营有其自身的弱点，难以抵御“两个风险”，发展农村合作组织是提高农民组织化程度，增强农民抗御“两个风险”能力，加快农业结构调整，实现农民收入稳定增长的客观要求和必然选择。目前，我国农村合作组织中仍然存在着资产不清、产权不明、管理混乱、管理队伍素质较低等一系列问题。新形势下我国农村合作组织发展需要在转变观念的基础上，因地制宜的采取切实有效的措施。