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131.
132.
TONG Xia-sheng LI Chang-chong RUAN Zheng-ying CHEN Hao GUO Hai-yuan FAN Guang-min 《园艺学报》2006,22(7):1397-1401
AIM:To observe the changes of polymorphonuclear leukocytes (PMN), neutrophil elastase (NE) and myeloperoxidase (MPO) expression in rat asthma. METHODS:Eighteen rats were randomly divided into two groups on average, including asthma group and control group. The rat model of asthma was established by the ovalbumin (OVA) challenge methods. Blood PMN were isolated andpurified. The protein expression of MPO were detected by immunohistochemistry and chromatometry techniques. NE was detected by ELISA. RESULTS:(1) Immunohistochemistry showed that the levels of MPO expression around bronchus and in purified PMN in asthma group were significantly increased compared with those in control group (P<0.01). Activities of MPO were significantly increased in bronchoalveolar lavage fluid (BALF) and lung tissue in asthma group compared with those in control group (P<0.05, P<0.01). (2) Levels of NE were significantly increased in BALF and PMN in asthma group compared with control group (P<0.01). (3) Number of PMN were significantly higher in BALF, bronchus and lung tissue in asthma group than those in control group (P<0.01). CONCLUSION:Levels of PMN counting and expression of MPO and NEare significantly increased in experimental asthma rats. PMN may be involved in inflammation in asthma via increasing the levels of NE and MPO. 相似文献
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AIM:To investigate the molecular mechanisms of apoptosis and to elucidate the apoptosis signaling pathway triggered by etoposide in Jurkat human leukemia cells. METHODS:Apoptosis was detected using annexin V-FITC and propidium iodide (PI) staining, respectively, and annexin V-FITC positive cells and hypodiploid cells were analyzed by flow cytometry. Mitochondrial membrane potential (△Ψm) was detected using 3, 3-dihexyloxycarbocyanine iodide [DiOC6(3)] staining and △Ψm low cells were analyzed by flow cytometry. Preparation of cytosolic extracts and isolation of mitochondria were completed by centrifugation. Western blotting analysis was used to evaluate the level of cytochrome c, caspase-3, and poly (ADP-ribose) polymerase (PARP) expression. RESULTS:Etoposide induced apoptosis showing phosphatidylserine externalization and DNA fragmentation in a time-dependent manner and the apoptosis could be inhibited by a broad caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (zVAD.fmk). Collapse of △Ψm induced by etoposide preceded DNA fragmentation and phosphatidylserine externalization. In contrast, it was not blocked by zVAD.fmk. Etoposide caused cytochrome c release from mitochondria into cytosol, subsequent activation of caspase-3 (32 kD) presented with an intermediate (20 kD) and its active product (17 kD), and cleavage of full-length PARP (116 kD) into the so-called apoptotic 85 kD fragment. CONCLUSION:Etoposide-induced Jurkat cell apoptosis is initiated through mitochondria signaling pathway with cytochrome c release into cytoplasm and caspase is the ultimate executioner of cell apoptosis. 相似文献
135.
抗香蕉枯萎病菌拮抗菌的鉴定及其定殖 总被引:4,自引:0,他引:4
通过逐步提高药物浓度方法,获得了抗利福平400μg/ml、对香蕉枯萎病菌拮抗活性稳定的T2WF和W10标记菌株。初步确定两菌株为芽孢杆菌属细菌。采用灌根接种法,在香蕉苗根际土和香蕉苗体内定殖结果表明,拮抗菌液接种浓度为5×104cfu/ml,两菌株均可从香蕉根际土和香蕉体内分离到,并在香蕉体内定殖和传导。在香蕉根际土、根部、球茎和假茎中,T2WF标记菌的菌量高峰分别出现在接种后5、5、11和3d,分别为707、437、273、117cfu/mg;而W10标记菌的菌量高峰分别出现在接种后5、3、5和5d,分别为784、260、253、110cfu/mg。两菌株在香蕉根际土和香蕉体内1~15d的消长动态均有一个共同的“由升到降”趋势。从数量和数量变动幅度上看,香蕉根部的菌量明显高于茎部的菌量。 相似文献
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138.
“母语语音负迁移”是中国学生在英语学习中普遍遇到的问题,其消极影响是显而易见的。本文从探讨汉语拼音的发音方法对英语语音的负迁移现象入手,揭示英汉语音的相似性与差异性。对英汉语音相似性与差异性的认知能促使中国学生在说英语时有意识地进行自我纠音,因此笔者认为在语音教学实践中引入英汉语音对比的相关知识及其研究成果将能有效地降低中国学生母语语音对目的语的干扰作用,从而提高语音教学质量。 相似文献
139.
阮晓菁 《福建农业大学学报(哲学社会科学版)》2009,(6):70-73
在和平共处五项原则的指导下,中国在外交上取得了丰硕成果,为国内各项建设事业提供了良好的外部环境。和平共处五项原则仍然是当今处理国际关系必须遵循的基本准则。中国要按照大国是关键、周边是首要、发展中国家是基础、多边是重要舞台的外交总布局发展同所有国家的友好合作关系。 相似文献
140.
新型自走式割灌机结构分析 总被引:1,自引:0,他引:1
介绍了一种新型自走式割灌机.该机由行走、割灌、驾驶台及排障四个核心部分组成,可以锯切直径为10~80 mm的灌木.本文对其行走部分和驾驶台进行了系统介绍,并对其主要部件进行了参数设计. 相似文献