大豆害虫天敌昆虫的研究概况

根据近年来的研究现状，对大豆害虫天敌昆虫的研究概况进行了综述，并对其发展趋势加以展望，以期为广大农业，植物保护工作者提供参考。     

磁化水渗灌装置

渗灌就是采用地下供水方式对农田进行灌溉。其基本特点是工作压力低,供水流量小,次数频繁,能精确控制灌溉用水量,只湿润作物根系附近的土壤,具有节水、节能、增产的效果。而在农业上用磁化水灌田,可使土质疏松,加快有机肥分解,刺激农作物生长。为此,全面、系统地介绍了磁化水渗灌装置的组成和磁化水渗灌的优点。     

塑料薄膜覆盖晾晒亚麻技术初步研究与应用

本文研究分析了塑料薄膜覆盖晾晒亚麻技术的效应原理;较详细地介绍了主要技术措施;阐述了该技术在当地的应用情况.本项技术对亚麻生产具有一定的积极意义.     

农业经济可持续发展初探

随着经济全球化和知识经济时代的来临,人类对农业生产的认识在不断提高和深化,农业经济可持续发展已成为当今世界的热点问题。本文运用可持续发展和复合生态系统理论,采用生态学和经济学的基本原理和方法,立足农业资源条件及所存在的问题,围绕农业经济可持续发展这一主题,探讨持续农业发展的方向、途径和具体对策。     

家蚕18项血液生理生化性状的杂种优势分析

测定了家蚕 3对现行品种及其杂交种的 18项血液生理生化指标 ,计算其杂种优势指数。发现 13项指标呈正负超亲优势 ,其中 :葡萄糖含量有较高的正超亲优势 ;钙、无机磷及球蛋白含量与乳酸脱氢酶活性基本没有杂种优势 ;谷丙转氨酶等 8项性状表现正杂种优势的趋势较强 ;尿酸等 4项性状则表现负超亲优势的趋势较强。按代谢类型分 :蛋白质代谢相关的 9项性状中呈现正负超亲优势的有 5～ 8项 ,占 5 5 .6 %～ 88.9% ;能量代谢相关的 6项中有 2～ 5项 ,占 33.3%～ 83.3% ;脂代谢相关的 2项中有 1项呈负超亲优势 ,占 5 0 %。表明家蚕经济性状广泛的杂种优势有其普遍的生理生化基础     

Advance in receptor type protein tyrosine phosphatase Q

Receptor type protein tyrosine phosphatase Q (PTPRQ) is an unusual protein tyrosine phosphatase that has intrinsic dephosphorylating activity for various phosphatidylinositiol and phospho-tyrosine substrates, especially the phosphatidylinositol activity. Recent data show that PTPRQ has an important role in various biological processes and is associated with some diseases. In this article, the structure and function of PTPRQ and the relationship between PTPRQ and diseases were briefly summarized.     

金花菜新品种‘淮扬金花菜’

‘淮扬金花菜'是通过对江苏扬州、镇江等地种质资源调查并搜集整理,经多年筛选出的新品种,具有蛋白质含量高、品质好、产量高、再生性好、抗逆性强,综合性状优良的特点。粗蛋白含量29.41%,粗灰分含量7.94%,中性洗涤纤维含量21.71%,酸性洗涤纤维含量10.29%,适宜多次刈割,鲜草产量30 000 kg·hm~(-2)以上,适宜在长江中下游地区保护地或露地秋季种植。     

利用同位素~(32)P示踪技术测定西伯利亚红松接穗与砧木适应性和亲和性的研究

利用同位素32P示踪技术,研究嫁接后西伯利亚红松树体内营养的分配、吸收与运输情况,评价接穗与砧木适应性和亲和性。结果表明:嫁接树的营养吸收明显大于非嫁接树,油松-西伯利亚红松嫁接树营养物质交换最好,是适应性较强的组合,这与引种组长期嫁接试验的生长势观察记录相一致。     

Down-regulated BARF1 expression induces EBV-positive gastric carcinoma cell apoptosis via activating caspase-dependent mitochondrial pathway

AIM: To investigate the effects of BARF1 down-regulation on EBV-positive gastric carcinoma cell apoptosis, and the molecular mechanisms by BARF1 silencing-mediated apoptosis. METHODS: After NUGC3 and SNU719 cells were transfected with NCsiRNA and siRNA, respectively, the protein levels of BARF1, Bcl-2, Bax, cytochrome C, caspase 3 and capase 9 were detected by Western blot, and the mRNA expression of BARF1, Bcl-2 and Bax was determined by RT-PCR. The cell viability was measured by the method of Trypan blue exclusion and the cell apoptosis was analyzed by flow cytometry analysis with Annexin V-FITC/PI staining. The expression of the apoptosis-related proteins in the cells transfected with siRNA and NCsiRNA was examined by human apoptosis antibody arrays. Mitochondrial membrane potential was determined by flow cytometry. The interaction between Apaf-1 and caspase 9 was confirmed by immunoprecipitation. RESULTS: Compared with untreated and NCsiRNA groups, BARF1 gene silencing significantly inhibited the cell viability, induced apoptosis, and reduced the mitochondrial membrane potential in the NUGC3 and SNU719 cells transfected with siRNA. BARF1 gene silencing up-regulated the expression of pro-apoptotic proteins and down-regulated the expression of anti-apoptotic proteins, and the Bcl-2/Bax ratio was significantly decreased. In BARF1 gene silencing cells, the caspase inhibitor z-VAD-fmk inhibited BARF1 silencing-mediated apoptosis, and significantly increased the levels of cleaved caspase 3 and caspase 9. The concentration of cytochrome C significantly increased as compared with NCsiRNA group, and Apaf-1 interacted with caspase 9 in the cytoplasm. CONCLUSION: BARF1 silencing induces apoptosis via the mitochondrial pathway through regulating the expression of Bcl-2 and Bax proteins in a caspase-dependent manner in the NUGC3 and SNU719 cells.