Effects of activated state of T cells from human peripheral blood on absorption of photosensitizer hematoporphyrin monomerthyl ether

AIM: To investigate the characterization of absorption of hematoporphyrin monomerthyl ether (HMME), a domestic new generation photosensitizer product, by activated T cells from human peripheral blood. METHODS: Evaluation was performed by flow cytometry on the effects of incubating concentration and time of HMME on absorption by activated T cells. Lymphocytes were separated from human peripheral blood by density gradient centrifugation with Ficoll and T cells were activated with polyclonal stimulators PHA and PDB+Ion. To analyze the effects of HMME incubating doses on the absorption of activated T cells, the cultural lymphocytes were incubated with a serial doses of HMME for 1 h and HMME absorption were measured by FACS after immuno-staining with anti-CD3 antibody. To test the impact of HMME incubating time on the absorption of activated T cells, the cultural lymphocytes were incubated with HMME for various times and HMME absorption were measured by FACS after immuno-staining with anti-CD3 antibody. RESULTS: The HMME absorption-dose curve and absorption-time curve were shifted to right and up in the activated T cells as compared to resting T cells. HMME absorptions of activated T cells were statistic significantly larger than that of resting T cells in the doses between 5 mg/L to 20 mg/L. HMME absorptions of either activated T cells or resting T cells underwent a gradual increase with the incubation-time in HMME at concentration of 10 mg/L. HMME absorptions of activated T cells were statistic significantly larger than that of resting T cells in the incubation-time between 15 to 60 min. CONCLUSION: The differences of HMME absorption between activated T cells and resting T cells depend on the incubation times and doses of HMME. HMME absorption of activated T cells are significantly larger than that of resting T cells in certain incubation-times and doses. These results suggest that incubation time and dose associated with HMME-PDT therapeutic windows will be created for selective deletion of activated T cells.     

Relationship between change of hypoxic tissue and vascular proliferation after cerebral infarction in rats

AIM: To clarify the relationship between the change of hypoxic tissue and vascular proliferation after cerebral infarction. METHODS: Rats with middle cerebral artery occlusion were randomly divided into 1.5 h ischemia-reperfusion (1.5 h IR) group and permanent ischemia (PI) group. Double staining by immunofluorescence was used to identify the hypoxic tissue and microvessels by EF5 and vWF labelings, respectively. On 3 d, 7 d and 14 d post-operation, microvessel density (MVD) and hypoxic tissue in ischemic cortex were observed. RESULTS: Hypoxic tissues existed from 3 d to 14 d post-operation in 1.5 h IR group and disappeared after 3 d in PI group. The MVD within hypoxic tissue was smaller than that around it at all observed time points (all P<0.01). With the lapse of time, MVD in ischemic cortex gradually increased both in 1.5 IR and PI groups (all P<0.01) and was bigger in 1.5 h IR group than that in PI group on 7 d and 14 d (both P<0.01). CONCLUSION: Hypoxic tissues mainly locate in areas of sparse microvessel after cerebral infarction and their duration is partially dependent on the grade of microvessel proliferation in ischemic cortex.     

应用CPV林间防治越冬代马尾松毛虫试验

应用CPV林间进行越冬代马尾松毛虫防治试验,结果表明不同剂量CPV与1/100000的20%氰戊菊酯乳油复配能显著提高CPV的林间杀虫效果。林间以CPV2.4×106~1.5×105多角体/ml与1/100000的20%氰戊菊酯乳油复配防治越冬代马尾松毛虫为宜,13天幼虫死亡率可达85%以上。     

猪瘟病毒持续感染对母猪繁殖性能及仔猪猪瘟疫苗免疫效力的影响

利用来源于同一猪场的2头猪瘟病毒(HCV)持续感染的带毒母猪及所产35头仔猪(包括13头死胎)和6头阴性对照猪,观察母猪的胎儿发育成活状况、仔猪HCV带毒率及HCV垂直传播对仔猪猪瘟兔化弱毒疫苗(HCLV)免疫效力的干扰作用,同时进行水平传播试验和观察HCV持续感染对母猪繁殖功能的影响。结果表明：HCV持续感染对其中1头母猪的胎儿发育和成活有明显影响,而对另1头母猪的胎儿发育没有明显影响;HCV持续感染母猪可经过胎盘垂直传播病毒给仔猪,传播率达45％～86％;吃初乳和接种HCLV不能阻止带毒仔猪的死亡,9头带毒仔猪在45d内死亡4头;免疫HCLV不能使带毒仔猪产生免疫保护力。5头猪在强毒攻击后死亡4头;HCV垂直传播的带毒猪可发生水平传播,并引起3／4感染猪死亡;HCV持续感染可引起母猪生殖系统病理变化。导致繁殖障碍。     

生物有机肥对马铃薯根际土壤生物活性 及根系活力的影响

针对大量化肥长期施用对马铃薯田土壤造成的生物活性降低等问题,利用根际益生菌（PGPR）制成生物有机肥,通过盆栽试验,研究了不施肥（对照,CK）以及分别施化肥（CF）、普通有机肥（OF）和5种生物有机肥（BOF1,BOF2,BOF3,BOF4和BOF5）对马铃薯根际土壤生物活性和根系活力的影响。结果表明,在马铃薯的成熟期,5种生物有机肥处理的可培养细菌数量平均比CF及OF高52%和37%,微生物量碳比CF和OF处理平均增加了30%,其中以BOF3效果最明显,比其它生物有机肥处理的可培养细菌数量和微生物量碳增加达3%～7%和2%～7%;且土壤脲酶、蔗糖酶和磷酸酶活性显著提高(P<0.05),相比CF和OF,5种生物有机肥处理土壤酶活性的增幅为11%～114%;根系活力分别增加了265%和224%,块茎产量分别增加了16%和21%,根系活力和块茎产量的提高也以BOF3效果最明显,其根系活力比BOF5高出166%,其块茎产量比其它生物有机肥处理的增幅为5%～9%。说明生物有机肥有助于提高土壤的生物活性,改善马铃薯的根际环境,进而提高了马铃薯的根系活力,增加了马铃薯的块茎产量。     

野生垂穗披碱草种质资源UV-B抗性综合评价

为探究垂穗披碱草(Elymus nutans)抗UV-B辐射能力强弱,筛选出优异种质材料,本研究以9份来自我国不同地区的野生垂穗披碱草为对象,研究其苗期UV-B辐射下的生长特性与生理特性,对其抗UV-B辐射能力进行综合评价。结果表明：随着UV-B辐射程度的加剧,9份材料的生长特性与生理特性均不同程度地受到影响。可将9份材料分为强、中、弱抗UV-B辐射材料。强抗材料为QH009,该材料受UV-B辐射影响最小,叶片受损程度最低,相对含水量下降幅度最小,细胞膜系统指标上升幅度最小,渗透调节物质积累最多,抗氧化系统酶活性显著高于其余材料(P<0.05),次生代谢物积累最多,光合系统指标下降幅度最小,可作为垂穗披碱草新品种选育与利用的基础材料。     

Ethanol extract from Cortex Albiziae attenuates mouse acute liver injury induced by carbon tetrachloride via modulation of NF-κB pathway

AIM:To investigate the protective effect of ethanol extract from Cortex Albiziae on acute liver injury, and to explore its possible mechanism. METHODS:Acute liver injury in mice was induced by single intraperitoneal injection of 25% carbon tetrachloride (olive oil solubilization). The effective parts of ethanol extract from Cortex Albizziae against acute liver injury were screened. The pathological changes of the liver tissues were examined by pathological sections with HE staining. The activity of total superoxide dismutase (T-SOD) and the content of malondialdehyde (MDA) of the liver tissues were detected, the serum levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were mea-sured by ELISA, and the protein expression levels of NF-κB p65, Bcl-2 and Bax in the liver cells of the mice in each group were determined by Western blot. RESULTS:Compared with model group, the serum levels of AST and ALT in low-dose n-butanol phase of ethanol extract from Cortex Albiziae (AB-L, 4 mg·kg^-1·d^-1) group and high-dose n-butanol phase of ethanol extract from Cortex Albiziae (AB-H, 8 mg·kg^-1·d^-1) group were significantly decreased. The necrosis extent and degree of the hepatocytes and infiltration of inflammatory cells were significantly lower than that in model group. Compared with model group, the serum levels of TNF-α and IL-6 in AB-H group and AB-L group were significantly decreased (P<0.05). The protein level of NF-κB p65 in the nuclei of mouse liver cells in AB-H group and AB-L group were also decreased significantly (P<0.05). Compared with model group, the protein expression of Bax was decreased, the protein expression of Bcl-2 was increased, and the Bcl-2/Bax ratio was increased in AB-L group and AB-H group. CONCLUSION:The n-butanol phase of ethanol extract from Cortex Albiziae may protect the liver by reducing the activation of NF-κB p65, inhibiting the excessive release of inflammatory cytokines IL-6 and TNF-α, and decreasing hepatocyte apoptosis via regulating Bcl-2 and Bax expression.     

豚草条纹叶(虫甲)(鞘翅目:叶(虫甲)科)的寄主专一性测定

1987年从苏联、加拿大引进原产北美的豚草天敌—豚草条纹叶(虫甲),在室内用具有代表性的23科72种主要经济作物、粮食作物、观赏植物及与豚草近缘或形态上相似的植物,进行了食性测定。结果表明,豚草条纹叶(虫甲)食性专一,仅取食豚草,不取食其它任何供试植物,在我国可以安全利用。在饥饿条件下,一龄幼虫的耐饥能力为3.1天,雌、雄成虫的耐饥能力分别为14和10.2天。     

转人血清白蛋白基因奶牛多重PCR快速检测方法的建立

建立转基因奶牛多重PCR快速检测方法,为转基因动物及产品进出境监测技术平台的建立提供技术支持,并为转基因动物及产品检测技术标准的制定提供参考。根据转基因奶牛内源基因β2-微球蛋白基因(β2-microglobulin,B2 M),外源基因人血清白蛋白基因(Human α-lactalbumin,α-LA)及标记基因绿色荧光蛋白基因(EGFP)和新霉素磷酸转移酶基因(NPTII)设计特异性引物,优化反应条件,建立转基因奶牛多重PCR检测方法。该方法敏感、快速、特异,一个反应可以检测多个基因片段,可有效用于转基因奶牛外源基因的检测。