江南地区墨西哥饲用玉米特性特征及栽培技术

根据多年的试验研究成果,系统介绍了墨西哥饲用玉米（Euchlaena mexicana Schrad）在江南地区的植物学、生物学特征及栽培技术要点等,以利推广种植。     

福建省牛病毒性腹泻病的血清学调查

为了了解福建省牛感染牛病毒性腹泻(BVDV)的情况,本中心对福建省9个地区2006-2008年的15个非免疫牛场和56个散养户共计511份样品进行了该病的检测。结果规模场BVDV血清学阳性一直保持在较高的水平,年平均阳性率维持在80%以上,散养户BVDV血清学阳性,2006、2007、2008年的血清学阳性分别为12.3%,21.4%和18.5%。调查表明,我省规模牛场感染BVDV较为严重,散养户感染BVDV的数量在逐年增加。     

Mitral annulus velocities and time intervals for evaluation of global left ventricular diastolic function in patients with coronary artery disease

AIM: To detect and compare the longitudinal mitral annulus diastolic velocity and time interval changes by pulsed Doppler tissue imaging (DTI) in patients with angina pectoris (AP) and myocardial infarction (MI), and to explore the value of mitral annulus diastolic velocities and time intervals for evaluation of global left ventricular diastolic dysfunction. METHODS: Fifty patients with established coronary artery disease were divided into AP group (16 cases) and MI group (34 cases). Sixteen age-matched healthy individuals served as the control group. The septum, lateral, anterior and inferior walls of the mitral annulus were displayed, and selected for DTI spectrum sampling. Peak early and late diastolic velocities and their ratio, time to the onset and peak of the early diastolic wave, and regional isovolumic relaxation time were measured, and the average values of the four mitral annular sites were calculated and presented as Em, Am, Em/Am, QEm, TEm and IVRTm, respectively. RESULTS: Compared with the control group, Em and Em/Am were significantly lower in both the AP and the MI groups (P<0.01). Em was even lower in the MI group than that in the AP group (P<0.01). QEm, TEm and IVRTm were significantly longer in the AP and the MI groups than those in control group (P<0.01 or P<0.05). IVRTm was even longer in the MI group than that in AP group (P<0.01). IVRTm had significantly negative correlation with Em (r=-0.64, P<0.01). CONCLUSION: Em, Em/Am, QEm, TEm and IVRTm as measured by pulsed DTI may be promising indexes for quantitative assessment of global left ventricular diastolic dysfunction in patients with coronary artery disease. Em and IVRTm may indicate the severity of ischemic myocardial damage.     

DIM enhances effect of cis-diamminedichloroplatinum on growth inhibition and apoptosis in human prostate cancer cell PC-3

AIM: To study the effects of the combination of cis-diamminedichloroplatinum(DDP) and 3, 3-diindolylmethane (DIM) on the growth and apoptosis of human prostate cancer cell PC-3. METHODS: MTT method was applied to detect the cell growth inhibitory rate. The cell apoptosis was measured by the flow cytometry and acridine orange staining method. The expression of the anti-oncogene p21 was detected by RT-PCR technique. RESULTS: The combination of 60 μmol·L^-1 DIM and 0.4 mg·L^-1 DDP effectively inhibited the growth and induced apoptosis in PC-3 cells. This result was the same as the effect of using 4 mg·L^-1 DDP only. The cell growth inhibitory and apoptosis rates for the combination of DIM and DDP were much higher than those for the individual effect. Both the combination and the single effect of these two medicines (i.e., DIM and DDP) all strengthen p21 mRNA expression significantly, and the effect of combination was more significant. CONCLUSION: DIM significantly enhances the effects of DDP on the growth inhibition and apoptosis induction in PC-3 cells.     

基于形态数据的大白菜核心种质构建方法的研究

 以国家蔬菜种质资源中期库收集保存的1 651份大白菜种质为试材,按照大白菜分类系统和生态型,采用分层分组法,将所有种质分为6组。基于43个形态性状的数据,比较了4种组内取样比例法、6种总体取样规模和2种取样方法在构建大白菜核心种质中的作用和效果。结果表明：组内以多样性比例法更能使各组的取样份数或比例趋于平衡,并较好地保持原始收集品的变异度。当总体取样规模为15%时,多样性比例法所构建的核心种质的遗传多样性指数达到最大,表型保留比例亦能达到98％左右;而当总体取样规模增加到20%以上时,虽然表型保留比例接近100％,但核心种质遗传多样性指数迅速降低。因此,认为15%总体取样规模较为合适。在一定的组内取样比例法和总体取样规模下,聚类取样构建的核心种质的遗传多样性指数（I）、表型保留比例（RPR）和变异系数（CV）均比随机取样的高。根据获得的优化方案最终在表型水平建立了包含248份种质的中国大白菜核心种质。     

茉莉花ISSR-PCR反应体系的建立

以茉莉花为材料,研究了PCR反应体系的主要成分及退火温度对茉莉花ISSR扩增结果的影响.结果表明:在20μL的反应体中,Mg2 的用量为1.2～1.6 mmol/L,dNTPs 浓度为0.15 mmol/L,引物的浓度为0.5μmol/L,Taq DNA 聚合酶的用量为1U,模板DNA的用量为40～70 ng,在48～50℃的退火温度下40个循环,能得到清晰、多态性高的ISSR带谱.     

聊红国槐谷胱甘肽过氧化物酶活性测定的研究

用直接测定法测定聊红国槐、国槐、龙爪槐、黄金槐样品谷胱甘肽过氧化物酶活性,其中以聊红国槐最高、龙爪槐活性最低.同时探讨了不同条件下的聊红国槐谷胱甘肽过氧化酶的活性高低,这一结果为今后研究该酶在国槐上的应用奠定了试验基础,为研究乔木树种中的GSH-Px活性情况提供理论依据.     

江西大富生态农业观光园总体规划

通过对大富生态农业观光园的指导思想、规划目标、功能分区、绿化配置诸方面,对其总体规划进行了介绍,力求将景观元素与农业生产相结合,使园区建设集观光、旅游、休闲、教育、生产、示范于一体.     

不同因素对贺兰山丁香愈伤组织诱导的影响

利用正交实验设计研究4种因素(6-BA、NAA、2,4-D、外植体)对贺兰山丁香愈伤组织诱导的影响,筛选出最佳的愈伤组织诱导条件为MS 6-BA 0.5 mg/L 2,4-D 0.1 mg/L,其中6-BA的浓度和外植体类型对贺兰山丁香种苗的出愈率有极显著影响(P=0.01).同时发现子叶和胚轴均可作为外植体材料,且以胚轴为外植体在最佳诱导条件下出愈率高达93.3%.     

优良观赏果树榅桲的推广应用

榅桲的栽培具有悠久的历史,它具有较强的耐寒、耐旱、耐盐碱能力,适于我国北方大多数城市栽培;其树姿、叶片、花和果实均具有较高的观赏价值,是较好的观赏树种;同时,榅桲又具有很高的经济价值,是优良的果树.因此,在我国北方,榅桲具有很高的推广价值.