超高效液相色谱法测定饲料中斑蝥黄和β-阿朴-8’-胡萝卜素酸乙酯含量方法的研究

为有效快速测定饲料中斑蝥黄和β-阿朴-8’-胡萝卜素酸乙酯含量,饲料经甲醇、正己烷和乙酸乙酯混合液萃取后,在氮气保护下浓缩,用甲基叔丁基醚-乙腈溶液复溶,注入超高效液相色谱仪中进行测定。结果表明:斑蝥黄浓度为1.25~10.0μg/mL,β-阿朴-8’-胡萝卜素酸乙酯为2.50~20.0μg/mL线性关系良好;斑蝥黄和β-阿朴-8’-胡萝卜素酸乙酯的检测限为0.06 mg/kg,定量限为0.20 mg/kg。斑蝥黄5个添加水平的平均回收率为72.8%~82.8%,相对标准偏差(RSD)为0.41%~1.06%;β-阿朴-8’-胡萝卜素酸乙酯5个添加水平的平均回收率为72.0%~82.8%,RSD为0.46%~1.41%。说明该方法简便、快速、灵敏度高、准确度好,适用于测定饲料中斑蝥黄和β-阿朴-8’-胡萝卜素酸乙酯含量。     

基于线粒体DNA Cyt b研究城市化对上海金线侧褶蛙遗传结构的影响

城市化在全球范围内快速扩张,已经成为影响物种进化的重要力量。两栖动物的迁移能力较弱,城市化导致两栖类被完全或部分的隔离,丢失更多的遗传多样性。本研究对上海市的金线侧褶蛙(Pelophylax plancyi)的mtDNA Cyt b部分序列进行分析,共扩增出235条序列,获得61个单倍型。研究表明城市区域遗传多样性较低,上海市的金线侧褶蛙分化程度处于中低的水平(Max F_ST=0.296;Min F_ST=0.004),各种群之间遗传分化有限。黄浦江及其支流可能作为水上扩散通道,有助于基因交流,各城市公园之间的遗传分化差异并不显著;而长江阻碍了种群之间的交流,城市公园和郊区,以及岛屿种群之间的遗传分化显著。此外,单倍型网络和系统发育分析表明,上海的金线侧褶蛙并没有对应城市化程度形成相应的单系。     

Flexure-torsion Analysis of Variable-curvature-box Girder Considering Non-coincidence of Torsion Center and Gravity Center

In order to consider the effect of non-coincidence of torsion center and gravity center,the rectangular variable-curvature-box girder with vertical symmetrical axis is studied. Considering the influence of shear deflection and non-coincidence of torsion center and gravity center, the elastic equilibrium formulas are established. Based on deformation equations of the girder and the relations of inner forces and deformations,the flexure-torsion differential equations are developed. The equilibrium differential equations and Galerkin methods formulas for clothoid curved box girder are presented.     

Investigation and Reference of the Mountain Tourist Hotel in Western Sichuan

Tourist hotel is the main supporting establishment for the touring industry.The authors investigated several important mountain beauty spots in the west of Sichuan province,some problems were found and their reasons were studied.Then,some common characters of mountain tourist hotels were summarized.It is hoped to give some reference to congenial buildings in west China in the future.     

A Method of Adaptive Fuzzy Classfication on Rockmass Blast Ability

This paper presents a series of targets which are usefull for engineering.The paper also presents an adaptive weight function according to the fail mechanism of rockmass and a fuzzy aynthetic evaluation medel on rockmass blast ability.The model is applied to evaluate the blast ability of rockmass in an engineering and the result shows that the model is effective.     

OPTIMAL DESIGN WITH WHOLE STRUCTURE PARAMETERS OF LOWER PART OF HUGE EXCAVATOR

A optimal design with whole structure parameters and its program are carried out. A reasonable model is established. Using the method a optimal design with whole structure parameters by original parameters of American P&H2300xp excavator was calculated. The size,carried out by the method,approximates to the size of P&H2300xp excavator very well.     

基于单台强震数据软厚场地地震反应不确定性分析

选取新西兰Canterbury地区12个强震台站及2010—2012年间的台站584条记录,以浅硬场地LPCC作为参考台站,系统分析Christchurch市区软厚场地地震效应(即场地放大函数)的不确定性及地震动强度、震级、震源距及震源方位角等因素对场地放大函数不确定性的影响规律。分析结果发现：1)地震动强度水平对场地放大函数均值影响较小,仅在长周期(如大于1.0 s)段趋于增大场地的放大效应;以不同地震动强度值进行分组,地震动强度大于0.6 m/s 2的分组场地放大函数标准差显著高于地震动强度小于0.6 m/s 2分组,特别是地震动强度大于1.0 m/s 2时场地放大函数标准差最大,反映了场地土层非线性提高场地地震效应的不确定性;2)地震震级对场地放大函数不确定性影响小,不同地震震级分组场地放大函数均值及标准差的表现基本相当;3)震源距是场地地震反应不确定性的一个重要因素,震源距小于5 km时场地放大函数的标准差明显大于其它情况,即震源距越小地震反应不确定性越大;4)场地放大函数不确定性与震源方位角的选取基本无关,即选取不同的震源方位角场地放大函数标准差无明显差别。     

Accomplishment of Elastic-plastic Analysis in Geotechnical FEA

In order to realize elastic-plastic analysis in geotechnical FEA, in this paper, firstly, the method how to realize elastic-plastic analysis in Super-Sap is discussed. Then, this paper stresses to introduce the principle and the steps used to achieve the accomplishment of elastic-plastic analysis in Super-Sap. Finally, an engineering practice is given to prove that the geotechnical FEA elastic-plastic analysis and visualized post-processing can come true by it rapidly.     

高港泵站水利枢纽自动化系统

详细介绍了江苏省泰州引江河高港泵站水利枢纽自动化系统的功能，介绍了系统采用的分布式体系结构、设备分布及其功能分配。     

夏秋反季节莴苣的营养吸收规律初探

对夏秋反季节莴苣营养吸收规律的试验结果表明,莴苣的生物量随莴苣的生长进程逐渐增加。移栽45 d后干物质积累量占全生育期的80%以上;氮、磷、钾的吸收最高峰出现在移栽后的45~60 d,吸收强度分别达22.47 mg/株.d、8.01 mg/株.d和35.61 mg/株.d。