浓核病毒研究概述

浓核病毒属于细小病毒科浓核病毒亚科,病毒颗粒为二十面体,无包膜,直径在18~30nm左右,基因组为单链DNA,末端具有发卡结构,大小为4～6kb,只感染无脊椎动物细胞。本文介绍了浓核病毒基因组的表达策略、宿主域及其决定因子、昆虫对浓核病毒的抗性机制,以及浓核病毒在卫生和农业领域潜在的应用价值。此外,也概括了浓核病毒对无脊椎动物养殖业潜在的威胁。     

采用Fluo-3 AM-ester探针研究桃不同组织的Ca^2＋信号分布

钙作为植物最重要的矿质元素之一,在植物生理和生化活动中起重要作用。采用激光扫描共聚焦显微镜,以Fluo-3 AM-ester为荧光探针研究了桃果肉和叶柄组织中Ca2＋的分布,结果表明Fluo-3 AM-ester可作为荧光探针对桃不同组织中游离的Ca2＋信号强度和分布特征进行分析。不同的物镜观察（10×和20×）下,C...     

CjFRO2在转基因枳中的整合与表达

三价铁螯合物还原酶（Ferric-chelator reductase,FCR）在植物对三价铁的吸收过程中起着关键作用,导入外源三价铁螯合物还原酶基因是改良植物耐缺铁能力的有效途径。香橙（Citrus junos Sieb.ex Tanaka）是目前表现最好的柑橘耐缺铁砧木之一,在铁胁迫时,香橙三价铁螯合物还原酶基因（...     

广西龙荔遗传多样性的ISSR分析

选用l2条ISSR引物组合分析了广西67个龙荔单株和10个龙眼品种（单株）、12个荔枝品种以及l0个野生荔枝单株的遗传多样性,均得到了清晰指纹。在所扩增的151条ISSR主带中,有127条具有多态性,多态性比率占84.11%。所用引物可将供试的品种（单株）区分开来。供试龙荔单株的遗传相似系数在0.61~0.97,表明广...     

硼对绵羊营养物质利用率的影响

选择健康的6月龄小尾寒羊与东北细毛羊杂交后代12只,采用单因素随机化试验设计,分成3组,每组4只羊。第1组对照组饲喂基础日粮,第2~3组分别在基础日粮中添加113mg.kg-1、226mg.kg-1硼(以硼砂形式添加),研究添加硼对绵羊营养物质利用率的影响。试验结果表明:粗蛋白质和能量的利用率均以补硼226mg.kg-1组为最高,分别比对照组高0.86%和11.14%,补硼226mg.kg-1对能量利用率的影响远大于对粗蛋白质利用率的影响;日粮中添加硼与对照组之间差异不显著(P>0.05)。钙、磷、锌、镁的利用率随着硼水平的增加而升高,而添加硼降低了铁的利用率。     

PAN树脂的制备及PAN树脂-微色谱柱分离富集ICP-AES法测定镉的研究

研究了PAN树脂的制备和PAN树脂—微色谱柱对镉的分离富集 ,然后用ICP -AES法测定镉。试验结果表明在pH10的缓冲溶液 (NH3 ·H2 O -NH4Cl)介质中 ,镉被树脂吸附 ,而样品中碱金属、碱土金属不被吸附 ,可用蒸馏水淋洗除去。再用 5mLφ =10 %热盐酸可完全解吸镉等元素 ,用ICP -AES法无干扰地测定ng·g-1级镉元素。     

红壤地油菜上施用的尿素态氮的去向及尿素在提高油菜产量中的效率

Fate of urea nitrogen(N) applied to rape grown on a red soil was investigated by the 15N mass balance technique.and efficiency of urea and effect of nutrients balance in raising rape yield were investigated in a field plot experiment.One hundred and thirty -eight kg N/ha,86kg N/ha as basal dressing and 52 kg N/ha as top dressing,was applied with band application technique.The experiment was conducted in the southeast of china ,near Yingtan City,Jianxi Province.Results from 15N mass balance study showed that when urea urea was applied as basal dressing the plant recovery was 44.0% of the applied N for Treatment T (with application of N,P,K,B and lime),Plant recoveries were 38.0%-40.5% for Treatments-K,-B-lime and RS(without application of K,B or lime as well as with additional rice straw compared with Teatment T),which were not significantly different from Treatment T.In contrast ,plant recovery was only 5.1% for Treatment-P(without application of P), indicating that P was the factor limiting N uptake by rape.However,N remaining in 0-0.30 m soil was high up to 71.6% for Treatment-P,while the corresponding data were 33.0%-42.6% for the other treatments.The total recovery of applied N(including plant recovery and N remaining in 0-0.60 m soil) was 91.5% for Treatment T when urea was applied as basal dressing,while almost all the applied N was recovered when ureawas applied as top drssing,It was suggested that N loss was greatly controlled by using band application method in this experiment.Results from the field plot experiment field plot experiment showed that N supply capacity of this red soil was very low,and the efficiency of the applied N was quite high,7.1kg rape seed was increased by application of one kg N for Treatment T.Nitrogen and phosphorus were the key factors limiting rape yield,and the yield was very low when neither of them was applied.The yield in Treatment-K was significantly lower than that in Treatment T,With the former accounting for 77% of the later.     

基坑土钉墙的数值模拟

通过数值模拟,对比实例工程的观测数据,讨论了土钉墙支护的基本规律、破坏模式、各排土钉发挥的作用、施工影响以及土层参数的影响,对设计和施工提出了建议。     

西藏中部农田土壤复退过程中的生物学特征

在西藏中部春播条件下,就不同培肥方式对土壤剖面微生物区系及其主要生理类群变化的影响,以及土壤微生物与土壤有机质、土壤养分的相互关系进行了研究。结果表明,细菌、放线菌、纤维素分解菌的土体差异较大,并主要集中于耕层;真菌、固氮菌的土体差异则极小。2～30,31～60cm土层土壤细菌在微生物区系构成中均占绝对优势,化肥对调控土壤细菌数量具有重要作用。不同培肥方式对2～30,31～60cm土层土壤真菌、固氮菌以及2～30cm土层土壤放线菌的繁殖普遍具有不同程度的抑制作用;2～30,31～60cm土层固氮菌与纤维素分解菌均呈负相关。土壤微生物与土壤有机质的相互促进作用较为明显,对提高土壤有效氮、有效磷亦具重要作用。     

La（Ⅲ）对UV—B辐射胁迫下大豆幼苗光氧化作用的影响

采用水培实验法研究了稀土La（Ⅲ）对紫外辐射（UV—B：0．15Wm^-2，0．45Wm^-2）胁迫下大豆（Glycinemax）幼苗体内O2^-和H2O2积累、抗坏血酸过氧化物酶（AsA—POD）活性变化和叶绿素（chl）光氧化降解的影响。实验结果表明：La（Ⅲ）可降低大豆幼苗体内的O2^-和H2O2含量、提高AsA—POD的活性，从而减轻光氧化作用对大豆幼苗chl的降解及对植株的伤害。