Progress in autoantibodies to platelet and idiopathic thrombocytopenic purpura

The onset of idiopathic thrombocytopenic purpura (ITP) is related to excessive destruction of platelet resulting from antiplatelet autoantibody. The immunity with ITP is imbalance, so the hypofunction of suppressor T cell (Ts) can't restrain B lymphocytes producing antibody, as a result, autoant ibody is produced, the clearance of platelet in blood circulation is accelerated. The different antibodies have different functions on ITP. The measuring of platelet antibody has its significance in diagnosis, therapeutic evaluat ion and expectation of prognosis.     

Effect of CGRP on level of lung endogenous NO in rabbits with acute lung injury

AIM:To examine whether calcitonin gene-related peptide (CGRP) enhances nitric oxide (NO) level in pulmonary circulation blood and observe the influence of CGRP on mean pulmonary artery pressure (mPAP) in rabbits with acute lung injury (ALI) caused by oleic acid.METHODS: The level of NO was assessed by measuring the presence of nitrite in cervical artery blood by the Griess reaction, mPAP was measured with right ventricular catheter.RESULTS:The level of nitrite in cervical artery blood was significantly increased and the mPAP was markedly reduced after administration of CGRP intravenousely.CONCLUSION:CGRP enhanced the NO level of pulmonary circulation blood and reduces the mPAP significantly in rabbits with ALI.     

咯菌腈对草莓灰霉病Botrytis cinerea的毒力及防效研究初报

用生长速率法测定了咯菌腈、多菌灵、硫菌·霉威、苯醚甲环唑4种药剂对草莓灰霉病Botrytis cinerea的毒力。结果表明,4种药剂对草莓灰霉菌菌丝的生长都有不同程度的抑制作用,随药剂浓度增加抑制作用增强,其EC50值分别为0.0472、95.7193、0.4854、1.3611 mg/L,其中咯菌腈的毒力最强。田间防治试验结果表明:2.5%咯菌腈FC每667 m2施药量120 mL时防效为83.7%,80 mL时防效为67.7%;65%硫菌·霉威WP每667m2施药量100 g时防效为65.3%。     

苏云金杆菌小试生产发酵影响因子的研究

利用 1 0L全自动发酵罐对苏云金杆菌小试生产的发酵条件进行了研究。结果显示 ,不同的温度、pH值、培养基成分及接种方式对发酵产量和发酵周期均有影响 ;在培养基配方中存在葡萄糖时 ,葡萄糖单独灭菌对提高苏云金杆菌发酵产量是重要的。初步生物测定显示 ,发酵上清中存在一些杀虫增效因子 ,建议配制苏云金杆菌制剂时尽可能加以利用。     

几种除草剂对水稻纹枯病菌的影响

研究了丁草胺、环庚草醚、草甘膦、苄嘧磺隆、吡嘧磺隆和环丙嘧磺隆6种除草剂对水稻纹枯病菌菌丝生长和菌核形成的影响。结果表明,在一定浓度范围内,6种除草剂均能抑制菌丝生长,减少菌核形成数量,且浓度越高,抑制能力越强,但在较高浓度范围内,除草剂可增加菌核体积;吡嘧磺隆在5μg/ml、环丙嘧磺隆在10μg/ml时可增加菌核形成数量;在含除草剂培养基上产生的菌核都可萌发。     

25s rRNA 基因部分序列比较在香菇栽培菌种鉴定上的应用

对目前中国7个主要香菇栽培品种：科，26，939，135，9311，申10和7402和25s rRNA基因中的D1／D2片段进行了DNA序列测定，根据DNA序列测定结果对它们之间的同源性进行了分析。结果表明，所测定的7个香菇栽培品种之间的亲缘关系非常近，同源性很高。通过聚类分析，根据香菇之间的亲缘关系，可以将这7个品种分为2类，即苏香，939和9311为一类，26，申10，135和7402为一类。     

强光下姜叶片的光呼吸及叶黄素循环

 姜叶片晴天中午的表观量子效率( AQY) 和PSÒ光化学活性(Fv/ Fm) 显著降低, 表明有明显的光抑制现象, 而干旱、高温、强光等逆境条件可显著加重光抑制程度。土壤水分胁迫使光呼吸速率( Pr) 降低, 峰值提前, Pr/ Pn 升高; 同时使叶黄素循环库增大, (A+ Z) / ( V+ A+ Z) 上升, 表明光呼吸和叶黄素循环是姜叶片耗散过剩光能的重要途径。     

早熟、优质、耐贮梨新品种新梨7号选育研究

以库尔勒香梨为母本,早酥梨为父本,有性杂交选育成新梨７号品种。该品种同时兼备优质、早熟与耐贮藏性;该品种遗传了父本的早熟性,比父本更早熟１５~２０ｄ,盛花期~果实可食期约８０~９５ｄ,属于早熟品种;且遗传了母本品质性状的质地酥脆、耐贮、清香、阳面红晕,比母本果个大,果心小。果柄木质化,丰产抗风。新梨７号果实贮藏期的可溶性固形物、果实重量、果实硬度和果实前期腐烂率的变化与库尔勒香梨基本相似,但耐热能力高于母本。凡适宜香梨、早酥、苹果梨和巴梨种植的地区,都适宜该品种的种植。     

Establishment of chronic obstructive pulmonary disease rat models by passive cigarette smoking and intratracheal instillation of lipopolysaccharide

AIM:To establish rat chronic obstructive pulmonary disease(COPD) models by passive cigarette smoking plus intratracheal instillation of lipopolysacchride(LPS) or passive cigarette smoking only, which would be similar to the pathogenesis of human COPD. METHODS:48 Wistar rats were randomly divided into 4 groups.(1) Healthy control I group(n=12), rats were bred 4 weeks;healthy control II group(n=12), rats were bred for 3months. (2) Model group I (n=12), 200μg lipopolysaccharide(LPS) was instilled intratracheally once for every two weeks and the rats were exposured to 5% of cigarette smoke, 0.5 h/d for 4 weeks.(3) Model group II(n=12),rats were exposed to 5% of cigarette smoke, 0.5 h/d for 3 months. The pathologic changes of airways and lung tissues, pulmonary function and blood gas analysis were determined. The airway wall lymphocytes and alveolar macrophages were counted. The cross areas of epithelial layer, smooth muscle layer and lamina propria of bronchi were measured. The hydroxyproline of lung tissue homogenates was determined by biochemistry method.RESULTS:The pathologic changes of airways and lung tissue of two models were similar to but milder than those of COPD patients(biopsy data). The collagen deposition and the cross areas of epithelial layer and smooth muscle layer in airway walls of two model groups were significantly increased than those of control groups(P＜0.01,P＜0.05).FEV_0.3/FVC% of two model groups, PaO₂ and SaO₂ of model I group were significantly decreased, while R_i and R_e in model I group were significantly increased than that of control I group(P＜0.05). The PaCO₂ and the counts of lymphocytes and alveolar macrophages of both model groups were significantly increased than those of the control groups (P＜0.01). Lots of alveolar macrophages had phagocyted smoke granules. The amounts of hydroxyproline of two model groups were significantly increased than those of control group((P＜0.05) and were negatively related to the FEV_0.3/FVC%, respectively (P＜0.01,P＜0.01) and positively related to airway resistance of model I group(P＜0.01). CONCLUSIONS:COPD rat models were successfully established by passive cigarette smoking plus intratracheal instillation of LPS and cigarette smoking only. The pathologic changes were similar but milder than those of COPD patients. The airway obstruction of model I group was more severe than that of model II group, but they have no significant difference.     

猪大肠杆菌水肿毒素SLT-IIeA基因的克隆和序列分析

研究以本地猪水肿病大肠杆分离物ED1株为材料 ,利用 PCR克隆了含猪水肿病大肠杆菌 (VTEC) slt-IIe A基因 987bp的片段 ,并测定了含该克隆片段的 Bam HI、Hind 酶切片段的核苷酸序列。结果表明 ,slt-IIe A基因的编码区全长 960 bp,编码 3 1 9个氨基酸的蛋白质 ,序列与国外报导的 S1 1 79株进行比较发现其核苷酸同源性为 98.9%。经推导的氨基酸序列的同源性为 99.7%。这为进一步研究 slt-IIe A的生物学特性、水肿病的分子诊断及其防制打下基础