超高效液相色谱-串联质谱法测定柑橘中的草甘膦及其代谢物残留

本研究首次建立了一种超高效液相色谱-串联质谱法(UHPLC-MS/MS)同时测定柑橘中草甘膦及其代谢物N-乙酰草甘膦?氨甲基磷酸(AMPA)和N-乙酰AMPA?样品经过0.5%甲酸水提取, 以十八烷基硅烷键合硅胶柱(C18)净化, 利用水和200 mmol/L碳酸氢铵溶液(含0.1%氨水)作为流动相梯度洗脱, 在多反应监测模式下定量分析?结果表明, 草甘膦及其代谢物在0.05~1 mg/kg范围内线性关系良好, 相关系数均大于0.99; 草甘膦及其3个代谢物在柑橘全果和果肉基质中3个加标水平下回收率为 70.5%~109.5%, 相对标准偏差(RSD)为0.6%~10.1%, 定量限为0.05 mg/kg?本方法的前处理样品无需衍生, 简便?快捷?高效?准确可靠, 可用于柑橘中草甘膦及其代谢物N-乙酰草甘膦?AMPA和N-乙酰AMPA的定量检测?     

机动车废气分析仪信号处理系统的智能化设计

提出了机动车废气分析仪的智能化组成及其实现信号处理的全过程。详细阐述了信号处理的软硬件的实现。实验结果表明：本系统克服了传统不分光红外线分析仪固有的缺陷。进一步保证了测量数据的正确性和准确性。     

棉花γ辐射诱变后代M5农艺经济性状的遗传变异

利用250Gy的Co60γ射线对棉花3个品种的干种子进行辐射处理,对其M5农艺经济性状的遗传变异进行分析。结果表明:3个品种的M5群体的铃重、单株铃数、株高、果枝数和麦克隆值的平均变异系数均超过10.0%以上,3个品种的辐射诱变后代M5群体表型性状变异系数存在明显差异;3个品种的M5群体的铃重与2.5%跨长和比强度均呈显著或极显著正相关,在其它性状间的相关性上3个品种的M5群体间存在较大差异;3个品种的M5群体的2.5%跨长均为第一主成分的主要因子。这阐明了辐射对不同棉花品种的诱变效果存在差异,揭示了辐射创造了丰富的遗传变异。     

不同花生品种侧枝发育动态与产量性状研究

花生一级侧枝发育具有不均衡性。第一、二对侧枝出现早，伸长快，其他一级侧枝出现晚，伸长慢。86036－26－1第一、二对侧枝发育相对较早，而其他一级侧枝发育较晚；海花1号和豫花1号第一、二对发育相对较晚，而其他一级发育较快；豫花7号和鲁花9号居中。海花1号一级侧枝及第一对侧枝的二级侧枝数最多，86036－26－1的一级侧枝数及第一对侧枝的二级侧枝数量少。在花针期之前第一对侧枝的伸长与主茎基本同步；花针期之后，第一对侧枝伸长速度超过主茎。86036－26－1的一级侧枝长及株高居首位。86036－26－1第一、二对侧枝饱果率，产量居第一，豫花7号居第二。海花1号虽然荚果数较多，但第一、二对侧枝孢果率较低，产量也较低；鲁花9号居中。     

The Research on the Construction of Concentrative Residence and Ecological Human Habitat Environment in the Rural Area of Rapidly Urbanizing Region

Under the background of the new rural construction in China, it is an important scientific question and an urgent social task to construct the harmonious society and to achieve the sustainable regional development on how to construct concentrative residence and manage the human habitat environment in the fast industrializing and typically urbanizing area. This paper analyzed the problems in the construction of the human habitat envi- ronment in the rural area of southern Jiangsu, discussed the methodologies of planning and design for rural con- centrative residence, researched the human habitat environment improvement technologies, and put forward the resolution of the recycling community for the rural inhabitation, which aimed at demonstrating the practicing in rapidly urbanizing regions.     

Biocompatibility of Acellular Porcine Dermis

In vitro cultured vascular endothelial cells (VEC) and mouse 3T3 fibroblasts (3T3) on the acellular dermal matrix , which were made of porcine skins. We made the cell proliferation test with MTT assay and the histological observation after cells were seeded on the acelluar dermis for 1 week with histological section. The cell growth curves showed VEC and 3T3 grew much rapidly on the acelluar dermis.The histological observation revealed VEC had formed a monolayer, some places even had formed 2 to 3 layer. The results suggest that the acelluar porcine dermal matrix have good biocompatibility , it will be widely applied.     

Research on Financing Plan for International Cooperative Project in Housing Industrialization

Housing industry is a prop industry with great potential, but it is restricted by shortage of funds. So it is necessary to probe the financing system of housing industrialization in China. This paper puts forward a financing plan for the international cooperative project in industrialized housing in Chongqing. Furthermore, it investigates the concrete financing system of the whole housing industry.     

神舟3号飞船搭载的树莓试管苗超微结构及其RAPD分析

已有研究表明,植物进入太空后,其生长、发育、细胞组织结构以及基因组均发生不同程度的变化.例如,大豆[1]及三叶草[2]受空间微重力的影响使其生长明显加快,许多植物的形态及细胞学行为也受太空环境影响[3～10];另外,空间微重力可以改变植物基因表达水平[11～14],半胱氨酸等放射性保护剂的应用进一步证实太空射线可损伤植物染色体[15].但目前有关太空习行对植物试管苗亚细胞超微结构及其基因组影响的报道较少.本文对神舟3号飞船搭载并成功回收的树莓试管苗进行了细胞亚显微结构及RAPD分子标记的初步分析.