Effects of capsaicin on cognitive impairment and mitochondria-associated endoplasmic reticulum membranes in rats with chronic cerebral hypoperfusion

AIM:To observe the effects of capsaicin on cognitive impairment and mitochondria-associated endoplasmic reticulum membranes (MAMs) of hippocampal CA1 area in the rats with chronic cerebral hypoperfusion (CCH), and to investigate the underlying molecule mechanism of cognitive defects induced by ischemia. METHODS:Healthy male Sprague-Dawley (SD) rats(n=48) were randomly divided into sham operation (sham) group,CCH model (CCH) group, capsaicin group,and solvent group, 12 rats in each group. Capsaicin at 2.5 mg/kg was intraperitoneally injected twice a week for 4 weeks, starting on the 7th day after surgery. The rats in solvent group were given the same amount of solvent at the same time and under the same conditions. Morris water maze, object recognition test and open field test were conducted to analyze the cognitive related behavior performance on the 4th week after surgery. The changes of MAMs in the hippocampal CA1 region were observed under transmission electron microscope, the co-localization of the MAMs was observed by immunofluorescence double-labeling, and the expression of mitofusin 2 (Mfn2) in the hippocampal tissue was determined by Western blot.RESULTS:Four weeks after the operation, the behavior tests showed that the cognitive function of CCH rats was impaired compared with sham operation group. Compared with solvent group, spatial learning and memory in capsaicin group was improved significantly. The results of transmission electron microscope and confocal microscope showed that the distance of MAMs in the hippocampal CA1 area of CCH rats was increased compared with sham operation group, and the co-localization of the contacts was decreased (P<0.05). Compared with solvent group, the correlation between the mitochondria and ER in capsaicin group was increased (P<0.05). The protein level of Mfn2 in CCH group was significantly lower than that in sham group (P<0.05). Compared with solvent group, the protein level of Mfn2 in capsaicin group was higher (P<0.05). CONCLUSION:CCH rats showed decreased cognitive function and loosen MAMs. Capsaicin improves the cognitive behavior of CCH rats by up-regulation of MAMs.     

F-box domain is involved in regulating the proliferation of MCF-7 cells by FBXO39 protein

AIM: To investigate the effect of F-box domain on the regulation of MCF-7 cell proliferation by FBXO39 protein. METHODS: The effect of F-box domain on the localization of FBXO39 protein in the MCF-7 cells was investigated. MCF-7 cell cDNA library was used as the template resource. The full-length cDNA sequence of FBXO39 was amplified by PCR method and subcloned into eukaryotic expression vector pEGFP-C2. The pEGFP-FBXO39ΔF (F-box domain deletion mutation) plasmid was successfully constructed with the template resource of pEGFP-FBXO39 plasmid. The recombinant plasmids were transfected into the MCF-7 cells, and then the expression of FBXO39 and FBXO39ΔF were determined by Western blot. The cellular localization of FBXO39 and FBXO39ΔF were observed by confocal microscopy. The localization of endogenous FBXO39 in the MCF-7 cells was detected by immunofluorescence staining. In addition, MTT and EdU assays were used to measure the cell proliferation, flow cytometry was used to measure the cell cycle distribution, and immunohistochemical staining was used to observe the expression of FBXO39 in the breast cancer and para-carcinoma tissues. RESULTS: The eukaryotic expression vector pEGFP-FBXO39 and pEGFP-FBXO39ΔF were constructed successfully. F-box domain had no effect on the cell localization of FBXO39. FBXO39 promoted MCF-7 cell proliferation but FBXO39ΔF did not. FBXO39 was highly expressed in the breast cancer tissues. CONCLUSION: F-box domain had no effect on the cellular localization of FBXO39 protein. However, it plays an important role in the biological function of FBXO39. FBXO39 may be related to breast cancer tumorigenesis.     

Efficacy of Soya Lecithin Powder Combined with Conventional Therapy on Chronic Hepatitis B

[Objectives]To probe into the treatment effects of soya lecithin powder combined with conventional therapy on chronic hepatitis B.[Methods]A total of 120 patien...     

增强病虫害防治能力提高森林资源的质量

针对江西森林病虫害发生现状、成因及防治存在的主要问题，阐述提高森林质量、增强病虫害防治能力的有效途径。     

桉树人工林中433MHz信道LoRa信号的传播特性

LoRa技术是最新出现的一种无线通信技术,具有低功耗、距离远、工作在非授权波段等特点,与ZigBee等现在常用的无线通信技术相比,其在人工林无线传感器网中更具潜力。然而由于LoRa的传输受环境影响较大,目前在人工林中传播特性的研究还处于空白。本研究基于Shadowing信号衰减模型,在广西东门林场中测试了433 MHz信道LoRa信号在两种天线高度下(1.5,2.0 m)和3种不同环境下(开阔环境、3 a林龄桉树人工林,5 a林龄桉树人工林)LoRa信号接收强度(RSSI)随距离变化的情况,每种条件下得到了两组数据。首先利用其中一组数据对模型进行拟合,拟合结果表明,LoRa信号传输遵从Shadowing模型,拟合度在0.886~0.982。然后利用另一组数据对模型的精度进行验证,验证结果表明,模型精度较高,模型的精度介于0.780~0.961。最后分析了桉树人工林中影响LoRa信号传输的主要因素。本研究的方法和获得的信号衰减系数可以为桉树人工林无线传感器网络监测中LoRa节点的组网和部署提供依据和参考。     

珍稀植物楠木的地理分布及潜在分布区的预测

以植物地理分布与气候变化间的关系为研究基础,通过MaxEnt模型,应用贡献率、置换重要值以及Jackknife检验、主成分分析,模拟研究影响楠木Phoebe zhennan地理分布的主要因子,分析楠木在当前的地理分布格局,并预测未来楠木在中国的潜在分布格局.结果表明,采用MaxEnt模型预测其潜在适宜分布区准确度极高,...     

免耕对华北地区潮土碳库特征的影响

以实施7年的中国科学院禹城综合试验站冬小麦-夏玉米轮作免耕长期定位试验场为对象,系统研究免耕条件下土壤总碳(TC)、有机碳(SOC)、无机碳(SIC)的变化,为进一步评价免耕措施对华北地区潮土碳库的影响提供数据支持。研究设置免耕秸秆覆盖(NTRC)、免耕施用有机肥(NTRR)、常规耕作(CT)3种处理,分析表层(0-20cm)及深层(20-60cm)土壤TC、SOC及SIC的变化特征和影响因素。主要结果为:NTRC和NTRR能够增加0-20cm土层TC含量及储量,但降低20-60cm土层TC含量及储量,0-60cm总碳储量表现为NTRC>CT>NTRR;与CT相比,NTRC能够显著增加0-20cm而降低20-60cm土层SOC含量及储量,NTRR增加了0-5cm土层SOC含量及储量,在5-60cm则呈降低趋势,0-60cm土层SOC储量表现为CT>NTRC>NTRR;NTRC增加了0-60cm土层SIC储量,而NTRR则影响较小。TC与SOC呈显著正相关(P<0.05),而与SIC呈显著负相关(P<0.05),说明总碳的变化趋势与SOC一致,与SIC相反。     

滇池流域农田土壤氮素流失影响因子研究

本试验采用田间原位模拟降雨并结合多元线性回归(逐步)的统计分析方法,分别在滇池流域的6个点位研究了旱季和雨季农田土壤的理化性质与氮素流失的关系。结果表明:各点位进行的模拟降雨的产流起始时间、产流历时和平均出水速度,在径流和渗漏两种流失方式下差异显著;两次径流试验中,质地为砂质黏壤土的C-2点(大渔乡元宝村)的初始产流强度和平均产流强度均最大。渗漏是氮素流失的主要方式,流失的形态主要是与NO3--N为主。土壤的孔隙度与降雨平均入渗率呈显著正相关,与径流中总氮(TN)和铵态氮(NH4+-N)的流失量呈负相关。0～20 cm的土壤硝态氮含量与地表径流和渗漏中总氮(TN)、硝态氮(NO3--N)的流失量呈极显著的正相关,是影响氮素流失的最重要因子,且0～20 cm土壤有机质含量与氮素的流失量呈正相关,土壤pH、5～20 cm的土壤含水量均与TN及NO3--N的流失量呈负相关。0～5 cm土壤铵态氮与NH4+-N的流失量呈正相关。     

百里香酚对鱼源耐药性维氏气单胞菌的体外抑菌效果及其机制研究

为探究百里香酚对鱼源耐药性维氏气单胞菌(Aeromonas veronii)的抑菌效果和作用机制,从患病黄颡鱼(Pelteobagrus fulvidraco)体内分离得到一株维氏气单胞菌,药敏试验结果显示,其对氟苯尼考、环丙沙星和恩诺沙星耐药。通过测定最低抑菌浓度(MIC)、最低杀菌浓度(MBC)和生长曲线来评价百里香酚对耐药性维氏气单胞菌的抑菌活性;通过测定百里香酚对其细胞膜通透性、可溶性蛋白、乳酸脱氢酶活性和DNA的影响,结合电镜观察细菌超微结构的变化研究其作用机制。结果显示,百里香酚对耐药性维氏气单胞菌的抑菌效果明显,MIC为256μg·mL^-1,MBC为512μg·mL^-1。经512μg·mL^-1的百里香酚作用1 h后,维氏气单胞菌液电导率极显著(P<0.01)上升,DNA外渗量迅速上升至(115.6±0.5)mg·L^-1。经512μg·mL^-1的百里香酚作用后,维氏气单胞菌的可溶性蛋白明显变少,细菌乳酸脱氢酶活性在2、4、6、8 h分别极显著(P<0.0...